全文获取类型
收费全文 | 391篇 |
免费 | 35篇 |
出版年
2022年 | 3篇 |
2021年 | 15篇 |
2020年 | 9篇 |
2019年 | 7篇 |
2018年 | 6篇 |
2017年 | 9篇 |
2016年 | 16篇 |
2015年 | 26篇 |
2014年 | 18篇 |
2013年 | 32篇 |
2012年 | 32篇 |
2011年 | 24篇 |
2010年 | 22篇 |
2009年 | 17篇 |
2008年 | 21篇 |
2007年 | 16篇 |
2006年 | 18篇 |
2005年 | 19篇 |
2004年 | 16篇 |
2003年 | 15篇 |
2002年 | 9篇 |
2001年 | 10篇 |
2000年 | 8篇 |
1999年 | 10篇 |
1998年 | 12篇 |
1994年 | 2篇 |
1990年 | 1篇 |
1989年 | 1篇 |
1988年 | 1篇 |
1987年 | 1篇 |
1986年 | 5篇 |
1985年 | 3篇 |
1984年 | 6篇 |
1983年 | 1篇 |
1980年 | 1篇 |
1979年 | 2篇 |
1976年 | 1篇 |
1975年 | 2篇 |
1970年 | 2篇 |
1969年 | 2篇 |
1968年 | 2篇 |
1966年 | 1篇 |
1964年 | 1篇 |
1951年 | 1篇 |
排序方式: 共有426条查询结果,搜索用时 31 毫秒
1.
2.
Nuclear Overhauser effect and computational characterization of the beta-spiral of the polypentapeptide of elastin 总被引:1,自引:0,他引:1
D K Chang C M Venkatachalam K U Prasad D W Urry 《Journal of biomolecular structure & dynamics》1989,6(5):851-858
The structure of the elastin polypentapeptide, poly(VPGVG), was studied by nuclear Overhauser effect experiments using perdeuterated Val1 and Val4 samples under the condition where intermolecular interactions are absent. More extensive interaction was found between the Val1 gamma CH and Pro2 beta CH protons than between the Val4 gamma CH and Pro2 beta CH protons. The Val1 gamma CH3-Pro2 beta CH interaction does not occur within the same pentamer as previously shown experimentally and as expected from steric considerations. The results are incompatible with the presence of a random chain network in poly(VPGVG) at room temperature but are readily explicable in terms of interturn interactions in a beta-spiral structure. More specifically, the results indicate that the beta-spiral conformation with 2.9 pentamers/turn is more prevalent than that with 2.7 pentamers/turn. Using conformations developed by molecular mechanics calculations, molecular dynamics simulations were carried out to compare the relative energies of these two variants of this class of beta-spiral structures. It was found in vacuo that the structure with 2.9 pentamers/turn is indeed more stable than that of 2.7 pentamers/turn by approximately 1 kcal/mole-pentamer. 相似文献
3.
Semi-empirical energy calculations for an internal Pro-Pro dimer are presented that take into account the nature of the flexibility of the proline ring due to its puckering. Calculations show that three stable conformations are available for the dimer: the cis (ω = 0°, ψ = 160°); the trans (ω = 180°, ψ = 160°, also referred to as trans′); and the cis′ (ω = 180°, ψ = ?40°) conformations. The best conformational pathways between these stable conformations are determined. Calculations also show that the barrier for cis′–trans′ conversion is of the same order of magnitude as that for cis–trans conversion. 相似文献
4.
5.
Kaur Jaspreet Kumar Vikas Sharma Kartik Kaur Sawinder Gat Yogesh Goyal Ankit Tanwar Beenu 《International journal of peptide research and therapeutics》2020,26(1):33-41
International Journal of Peptide Research and Therapeutics - Bioactive peptides have been reported to exhibit opioid-like activity and are termed as opioid peptides. Either these are produced... 相似文献
6.
Zhang Guanshi Zhang Jialing DeHoog Rachel J. Pennathur Subramaniam Anderton Christopher R. Venkatachalam Manjeri A. Alexandrov Theodore Eberlin Livia S. Sharma Kumar 《Metabolomics : Official journal of the Metabolomic Society》2020,16(1):1-12
Metabolomics - Food and dietary ingredients have significant effects on metabolism and health. To evaluate whether and how different diets affected the serum lipidomic profile of dogs. Sixteen... 相似文献
7.
Eben Goodale Hari Sridhar Kathryn E. Sieving Priti Bangal Gabriel J. Colorado Z. Damien R. Farine Eckhard W. Heymann Harrison H. Jones Indrikis Krams Ari E. Martínez Flavia Montao‐Centellas Jenny Muoz Umesh Srinivasan Anne Theo Kartik Shanker 《Biological reviews of the Cambridge Philosophical Society》2020,95(4):889-910
Mixed‐species animal groups (MSGs) are widely acknowledged to increase predator avoidance and foraging efficiency, among other benefits, and thereby increase participants' fitness. Diversity in MSG composition ranges from two to 70 species of very similar or completely different phenotypes. Yet consistency in organization is also observable in that one or a few species usually have disproportionate importance for MSG formation and/or maintenance. We propose a two‐dimensional framework for understanding this diversity and consistency, concentrating on the types of interactions possible between two individuals, usually of different species. One axis represents the similarity of benefit types traded between the individuals, while the second axis expresses asymmetry in the relative amount of benefits/costs accrued. Considering benefit types, one extreme represents the case of single‐species groups wherein all individuals obtain the same supplementary, group‐size‐related benefits, and the other extreme comprises associations of very different, but complementary species (e.g. one partner creates access to food while the other provides vigilance). The relevance of social information and the matching of activities (e.g. speed of movement) are highest for relationships on the supplementary side of this axis, but so is competition; relationships between species will occur at points along this gradient where the benefits outweigh the costs. Considering benefit amounts given or received, extreme asymmetry occurs when one species is exclusively a benefit provider and the other a benefit user. Within this parameter space, some MSG systems are constrained to one kind of interaction, such as shoals of fish of similar species or leader–follower interactions in fish and other taxa. Other MSGs, such as terrestrial bird flocks, can simultaneously include a variety of supplementary and complementary interactions. We review the benefits that species obtain across the diversity of MSG types, and argue that the degree and nature of asymmetry between benefit providers and users should be measured and not just assumed. We then discuss evolutionary shifts in MSG types, focusing on drivers towards similarity in group composition, and selection on benefit providers to enhance the benefits they can receive from other species. Finally, we conclude by considering how individual and collective behaviour in MSGs may influence both the structure and processes of communities. 相似文献
8.
Mandal Anup Mani Anjali Kottayil Lamech Ruban Anandajothi Elamaran Venkatachalam Shanmuga Arasu Dinakaran Gajendiran K. Quinitio Emilia T. Kandan Shanmuganathan 《Biochemical genetics》2021,59(4):856-869
Biochemical Genetics - Misleading identification and subsequent publications on biological, molecular, and aquaculture data of mangrove mud crab (genus Scylla de Hann 1833) is a major concern in... 相似文献
9.
Prakash Maran Jeganathan Sivakumar Venkatachalam Thirugnanasambandham Karichappan Sridar Ramasamy 《Preparative biochemistry & biotechnology》2013,43(1):56-67
The objective of the present study is to find out the optimum extraction conditions for extraction of polyphenols from red grapes using Box–Behnken design. Red grapes polyphenols were extracted using acid–ethanol solvent at various extraction temperature (40–60°C), extraction time (20–100 min) and different solid–liquid ratio (1:5–1:15 g:ml). The effect (main and interactive) of extraction conditions on total anthocyanin, phenolic and flavonoid content were studied using Box–Behnken design (three factors at three levels). The results showed that the contribution of the quadratic model was significant for all the responses. Second-order mathematical regression models were developed and were found to fit well with observed data. Derringer's desirability function methodology was performed to find out the optimal conditions based on both individual and combinations of all responses (extraction temperature: 57°C, time: 61 min, and solid–liquid ratio: 1:8.7 g:ml) were established. At this optimal condition, the anthocyanin yield, total phenolic and flavonoid content were 73.92 mg/100 g, 221.4 mg GAE/100 g, and 79.08 mg CE/100 g, respectively. A desirability value of 0.902 was achieved at this point. 相似文献
10.
Chen Chen Vengadesan Krishnan Kevin Macon Kartik Manne Sthanam V. L. Narayana Olaf Schneewind 《The Journal of biological chemistry》2013,288(41):29440-29452
Staphylococcus epidermidis, a commensal of humans, secretes Esp protease to prevent Staphylococcus aureus biofilm formation and colonization. Blocking S. aureus colonization may reduce the incidence of invasive infectious diseases; however, the mechanism whereby Esp disrupts biofilms is unknown. We show here that Esp cleaves autolysin (Atl)-derived murein hydrolases and prevents staphylococcal release of DNA, which serves as extracellular matrix in biofilms. The three-dimensional structure of Esp was revealed by x-ray crystallography and shown to be highly similar to that of S. aureus V8 (SspA). Both atl and sspA are necessary for biofilm formation, and purified SspA cleaves Atl-derived murein hydrolases. Thus, S. aureus biofilms are formed via the controlled secretion and proteolysis of autolysin, and this developmental program appears to be perturbed by the Esp protease of S. epidermidis. 相似文献