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Rudenko N. V. Karatovskaya A. P. Zamyatina A. V. Siunov A. V. Andreeva-Kovalevskaya Zh. I. Nagel A. S. Brovko F. A. Solonin A. S. 《Russian Journal of Bioorganic Chemistry》2020,46(3):321-326
Russian Journal of Bioorganic Chemistry - Hemolysin II (HlyII) is one of the pathogenic factors of Bacillus cereus. With respect to the prototype of β-barrel toxins, the α-toxin of S.... 相似文献
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Rudenko N. V. Karatovskaya A. P. Shepelyakovskaya A. O. Zamyatina A. V. Brovko F. A. Koltsov A. Yu. Imatdinov I. R. Imatdinov A. R. Strijakova O. M. Mima K. A. Lyska V. M. Kolbasov D. V. 《Russian Journal of Bioorganic Chemistry》2019,45(6):505-513
Russian Journal of Bioorganic Chemistry - Bluetongue, or catarrhal fever of sheep, is a viral vector-borne infection of ruminants, which is one of the economically significant arbovirus infections... 相似文献
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N. V. Rudenko I. M. Tsfasman O. R. Latypov L. A. Ledova L. A. Krasovskaya A. P. Karatovskaya F. A. Brovko N. V. Vasileva O. A. Stepnaya 《Russian Journal of Bioorganic Chemistry》2014,40(3):272-278
Extracellular lytic endopeptidases AlpA and AlpB of the Gram-negative bacterium Lysobacter sp. XL1 have a high degree of homology and are synthesized as preproproteins consisting of a signal peptide, a propeptide, and the mature protein. In the present work, two monoclonal antibodies against the AlpA propeptide (ProA) and eleven antibodies against the AlpB propeptide (ProB) have been obtained. The affinity constants for antibodies to ProA were 2.9 × 109 and 3.5 × 109 M?1, and those for antibodies to ProB were from 1.5 × 108 to 2.2 × 109 M?1. The antibodies showed no immune cross-reactivity with each other and with mature forms of the enzymes. On the basis of monoclonal antibodies, a sandwich enzyme immunoassay has been developed, which makes it possible to detect these propeptides in the dissolved native form. The linear range of the detection of ProA was 1.5–100.0 ng/mL with an error of measurement of 6%, and that of the determination of ProA was 0.2–6.25 ng/mL with an error of measurement of 6%. By using the assay, propeptides ProA and ProB were detected in cell lysates of Lysobacter sp. XL1 in an amount of 1.18 ± 0.03 and 0.096 ± 0.002 ng per 1 OD540 of bacterial culture, respectively. The immunochemical assay for the detection of different forms of AlpA and AlpB can be useful in solving the problems associated with their secretion into environment. 相似文献
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N. V. Rudenko A. P. Karatovskaya I. M. Tsfasman F. A. Brovko N. V. Vasilyeva 《Russian Journal of Bioorganic Chemistry》2017,43(5):526-530
Homologous endopeptidases AlpA and AlpB are components of the secreted complex of lytic enzymes of the Gram-negative bacterium Lysobacter sp. ХL1. These enzymes are synthesized as precursors that consist of a signal peptide, propeptide, and proteolytically active mature part. To understand the topogenetic features of these proteins, bacterial cell fractions were investigated by a sensitive sandwich enzymelinked immunosorbent assay and immunoblot analysis with the use of monoclonal antibodies recognizing unique epitopes of proteins’ mature forms and their propeptides. Only mature forms of the enzymes, without propeptides, were shown to be released outside the cell into the environment. AlpA significantly exceeds AlpB in the production level at the early stationary growth stage. The AlpB precursor was revealed in the cytoplasmic and periplasmic fractions, and the AlpA precursor was found only in the cytoplasmic fraction. The periplasmic fraction was also found to contain the mature forms of both enzymes and their propeptides. These results indicate that AlpA and AlpB are released into the environment through different mechanisms. AlpA is translocated across the cell envelope without being interrupted in the periplasm. The homologous AlpB enzyme, on the contrary, accumulates in the periplasmic space and is captured by outer membrane vesicles in the process of their formation. 相似文献
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Rudenko N. V. Karatovskaya A. P. Zamyatina A. V. Malogolovkin A. S. Oleinikov V. A. Brovko F. A. Kol’tsov A. U. Lapteva O. G. Kolbasov D. V. Shepelyakovskaya A. O. 《Russian Journal of Bioorganic Chemistry》2022,48(4):793-800
Russian Journal of Bioorganic Chemistry - Bluetongue is a reemerging transmissive infectious disease that threatens all countries with intensive livestock production. In cattle, bluetongue may... 相似文献
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Zamyatina A. V. Rudenko N. V. Karatovskaya A. P. Shepelyakovskaya A. O. Siunov A. V. Andreeva-Kovalevskaya Zh. I. Nagel A. S. Salyamov V. I. Kolesnikov A. S. Brovko F. A. Solonin A. S. 《Russian Journal of Bioorganic Chemistry》2020,46(6):1214-1220
Russian Journal of Bioorganic Chemistry - Among the panel of monoclonal antibodies to the recombinant protein HlyIICTD Bacillus cereus an antibody was found capable of forming an immune complex... 相似文献
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