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1.
MICHIKO WATANABE DONALD R. BERTOLINI J. RANDOLPH SCHNTTMAN ROBERT S. TURNER Jr. 《Differentiation; research in biological diversity》1982,21(1-3):79-85
Sea urchin embryos can be dissociated into a suspension of single cells that reconstitute embryo-like structures. When reconstitution is conducted in stationary cultures the first step is attachment of the cells to the culture plate, which requires calcium and metabolic energy but not protein synthesis. We have found that protease treated cells form cell-cell associations in stationary cultures without attaching to the culture plates, and that cell-plate attachments are unaffected by inhibition of protein synthesis. These data suggest that cell surface proteins are needed for cell-plate attachment and that these proteins are present on freshly dissociated cells. We also demonstrated that butanol extracted cells attach to the plates, but do not form functional cell-cell associations unless the butanol extracted material is restored to them. We conclude that sea urchin embryo cells contain two classes of attachment components. The first class functions in the cell-plate attachments, is protease sensitive, and not extracted by butanol; the second class is necessary for cell-cell associations, is protease insensitive, and extracted by butanol. Since protease treated cells reconstitute embryo-like structures without attaching to the culture plates, only the second class of attachment components is necessary for embryo reconstitution. 相似文献
2.
ROWLAND-BAMFORD AMANDA J; ALLEN L HARTWELL JR; BAKER JEFFREY T; BOOTE KEN J 《Journal of experimental botany》1990,41(12):1601-1608
The atmospheric carbon dioxide (CO2) concentration has beenrising and is predicted to reach double the present concentrationsometime during the next century. The objective of this investigationwas to determine the long-term effects of different CO2 concentrationson carbohydrate status and partitioning in rice (Oryza sativaL cv. IR-30). Rice plants were grown season-long in outdoor,naturally sunlit, environmentally controlled growth chamberswith CO2 concentrations of 160, 250, 330, 500, 660, and 900µmolCO2 mol1 air. In leaf blades, the priority between the partitioningof carbon into storage carbohydrates or into export changedwith developmental stage and CO2 concentration. During vegetativegrowth, leaf sucrose and starch concentrations increased withincreasing CO2 concentration but tended to level off above 500µmolmol1 CO2. Similarly, photosynthesis also increased withCO2 concentrations up to 500µmol mol1 and thenreached a plateau at higher concentrations. The ratio of starchto sucrose concentration was positively correlated with theCO2 concentration. At maturity, increasing CO2 concentrationresulted in an increase in total non-structural carbohydrate(TNC) concentration in leaf blades, leaf sheaths and culms.Carbohydrates that were stored in vegetative plant parts beforeheading made a smaller contribution to grain dry weight at CO2concentrations below 330µmol mol1 than for treatmentsat concentrations above ambient Increasing CO2 concentrationhad no effect on the carbohydrate concentration in the grainat maturity Key words: CO2 enrichment, starch, sucrose 相似文献
3.
Nuclear Receptors in Mosquito Vitellogenesis 总被引:1,自引:0,他引:1
RAIKHEL ALEXANDER S.; MIURA KEN; SEGRAVES WILLIAM A. 《Integrative and comparative biology》1999,39(4):722-735
Vitellogenesis in insects involves the coordinated activityof the fat body, which produces large amounts of yolk proteinprecursors (YP), and oocytes, which specifically accumulatethese proteins. The expression of YP genes is achieved throughstrict sex-, tissue-, and hormone-specific control in the femalefat body. In mosquitoes, expression of YP genes is controlledby 20-hydroxyecdysone (20E). To elucidate the role of 20E inmosquito vitellogenesis, we cloned cDNAs encoding the Aedesaegypti ecdysteroid receptor (AaEcR) and two isoforms of itsheterodimeric partner, the Ultraspiracle homologue (AaUSP).The two AaUSP isoforms differ in their A/B domains and havedistinct expression patterns. The ecdysone regulation of YPgenes likely involves products of early genes. We cloned thegene of the mosquito homologue to the Drosophila early geneE75 (AaE75) belonging to the nuclear receptor superfamily. Kineticsof AaE75 expression correlate with the expression of YP genes,suggesting that AaE75 may have a regulatory role in YP geneexpression. A second nuclear receptor superfamily member, theNGFI-B homologue AaHR38 is implicated in repression of the ecdysone-signalingpathway in the fat body of the previtellogenic female mosquitoat the state-of-arrest. Finally, three isoforms of the hepatocytenuclear factor 4 (HNF-4) homologue AaHNF-4 are differentiallyexpressed in the mosquito fat body during vitellogenesis, suggestingtheir involvement in regulating vitellogenic events in thistissue. 相似文献
4.
Abstract Dopamine has been suggested to be involved in physiological and/or behavioural changes triggered by mating in European honeybee (Apis mellifera) queens but its specific role remains unclear. In the present study, the amounts of dopamine (DA) and its metabolite, N‐acetyldopamine (NADA) are measured, in queens of various ages to clarify the association with locomotor activity. The effects of DA receptor agonist/antagonist drugs on locomotor activity are further investigated. Brain levels of DA and NADA are relatively constant during the period before mating when locomotor activity reportedly increases with age but decreases in 1‐year‐old laying queens with low locomotor activity. Reduced DA and NADA levels are also found in haemolymph of 1–3‐month‐old laying queens. When a DA receptor agonist or antagonist is injected into 6‐day‐old virgin queens, locomotor activity levels increase significantly with 2‐amino‐6,7‐dihydroxy‐1,2,3,4‐tetrahydronaphthalene (agonist), and decreased with cis(Z)‐flupenthixol (antagonist). These results suggest that DA systems are involved in the motor control of honeybee queens, and that the decline in DA levels reduces locomotor activity after mating but increased locomotor activity before mating may be independent of DA levels. 相似文献
5.
STIAN NYLUND ARE NYLUND KUNINORI WATANABE CARL E. ARNESEN EGIL KARLSBAKK 《The Journal of eukaryotic microbiology》2010,57(2):95-114
ABSTRACT. Paranucleospora theridion n. gen, n. sp., infecting both Atlantic salmon (Salmo salar) and its copepod parasite Lepeophtheirus salmonis is described. The microsporidian exhibits nuclei in diplokaryotic arrangement during all known life‐cycle stages in salmon, but only in the merogonal stages and early sporogonal stage in salmon lice. All developmental stages of P. theridion are in direct contact with the host cell cytoplasm or nucleoplasm. In salmon, two developmental cycles were observed, producing spores in the cytoplasm of phagocytes or epidermal cells (Cycle‐I) and in the nuclei of epidermal cells (Cycle‐II), respectively. Cycle‐I spores are small and thin walled with a short polar tube, and are believed to be autoinfective. The larger oval intranuclear Cycle‐II spores have a thick endospore and a longer polar tube, and are probably responsible for transmission from salmon to L. salmonis. Parasite development in the salmon louse occurs in several different cell types that may be extremely hypertrophied due to P. theridion proliferation. Diplokaryotic merogony precedes monokaryotic sporogony. The rounded spores produced are comparable to the intranuclear spores in the salmon in most aspects, and likely transmit the infection to salmon. Phylogenetic analysis of P. theridion partial rDNA sequences place the parasite in a position between Nucleospora salmonis and Enterocytozoon bieneusi. Based on characteristics of the morphology, unique development involving a vertebrate fish as well as a crustacean ectoparasite host, and the results of the phylogenetic analyses it is suggested that P. theridion should be given status as a new species in a new genus. 相似文献
6.
7.
KEN‐ICHI HARANO SEIJI TANAKA MAKOTO TOKUDA HIROE YASUI SADAO WAKAMURA ATSUSHI NAGAYAMA YASUHIRO HOKAMA NORIO ARAKAKI 《Physiological Entomology》2010,35(3):287-295
The present study investigates the emergence of adult white grub beetles Dasylepida ishigakiensis Niijima et Kinoshita (Coleoptera: Scrabaeidae) from soil as well as their burrowing behaviours. ‘Standby behaviour’ (i.e. adults come to the soil surface where they expose their heads) is shown in the field and, along with emergence behaviour, is entrained by LD photocycles. These 24‐h rhythms persist after transfer to continuous light conditions for 2 days. By contrast, beetles transferred from LD photocycles to continuous dark conditions fail to show standby behaviour; thus, it appears to be manifested only in the presence of illumination. Under dark conditions, beetles emerge completely from the soil directly at the time when standby behaviour is otherwise expected to occur. Emerged adults then burrow back into the soil before dawn. Virgin and mated males, as well as virgin females, which are expected to emerge from the soil for mating on later evenings, burrow to a relatively shallow depth (<2 cm), whereas mated females burrow deeper (2–10 cm). Soil properties such as moisture, grain size, topography and temperature influence the burrowing behaviour and the depths that the beetles reach. 相似文献
8.
就取食过程中惯用左手还是右手的问题,我们对安徽省黄山的一群短尾猴进行了研究。结果发现在调查的33只个体中,9只惯用右手取食,11只惯用左手取食,另外13只对左右手的使用没有偏好。而且短尾猴在左右手的偏好方面也没有性别或年龄的差异。以往的一些研究表明猕猴类动物习惯于用左手取食,但是研究群的短尾猴并没有表现出这种倾向。我们将短尾猴的研究结果与猕猴属其它种类进行了比较,并讨论了猕猴属在这一行为上的多样性。 相似文献
9.
10.
SAMUEL K. SHEPPARD FRANCES M. COLLES NOEL D.
McCARTHY NORVAL J. C. STRACHAN IAIN D. OGDEN KEN J. FORBES JOHN F. DALLAS MARTIN C. J. MAIDEN 《Molecular ecology》2011,20(16):3484-3490
Bacterial populations can display high levels of genetic structuring but the forces that influence this are incompletely understood. Here, by combining modelling approaches with multilocus sequence data for the zoonotic pathogen Campylobacter, we investigated how ecological factors such as niche (host) separation relate to population structure. We analysed seven housekeeping genes from published C. jejuni and C. coli isolate collections from a range of food and wild animal sources as well as abiotic environments. By reconstructing genetic structure and the patterns of ancestry, we quantified C. jejuni host association, inferred ancestral populations, investigated genetic admixture in different hosts and determined the host origin of recombinant C. jejuni alleles found in hybrid C. coli lineages. Phylogenetically distinct C. jejuni lineages were associated with phylogenetically distinct wild birds. However, in the farm environment, phylogenetically distant host animals shared several C. jejuni lineages that could not be segregated according to host origin using these analyses. Furthermore, of the introgressed C. jejuni alleles found in C. coli lineages, 73% were attributed to genotypes associated with food animals. Our results are consistent with an evolutionary scenario where distinct Campylobacter lineages are associated with different host species but the ecological factors that maintain this are different in domestic animals such that phylogenetically distant animals can harbour closely related strains. 相似文献