全文获取类型
收费全文 | 188篇 |
免费 | 20篇 |
国内免费 | 39篇 |
出版年
2023年 | 6篇 |
2022年 | 8篇 |
2021年 | 14篇 |
2020年 | 6篇 |
2019年 | 12篇 |
2018年 | 13篇 |
2017年 | 8篇 |
2016年 | 12篇 |
2015年 | 13篇 |
2014年 | 9篇 |
2013年 | 14篇 |
2012年 | 20篇 |
2011年 | 19篇 |
2010年 | 9篇 |
2009年 | 13篇 |
2008年 | 13篇 |
2007年 | 9篇 |
2006年 | 13篇 |
2005年 | 10篇 |
2004年 | 4篇 |
2003年 | 6篇 |
2002年 | 2篇 |
2001年 | 1篇 |
2000年 | 1篇 |
1999年 | 1篇 |
1998年 | 3篇 |
1997年 | 3篇 |
1996年 | 1篇 |
1994年 | 1篇 |
1989年 | 2篇 |
1988年 | 1篇 |
排序方式: 共有247条查询结果,搜索用时 0 毫秒
1.
青海藏族青少年骨龄与生长发育关系研究 总被引:1,自引:0,他引:1
本文报告了青海省境内,世居在海拔3000-4000米地区的728名7-18岁健康藏族青少年学生的手、腕部骨骼发育情况,对骨化中心出现和骨骺愈合求出了50%出现年龄,并对骨龄与青春期身高突增的关系及与月经初衬潮的关系进行了分析。 相似文献
2.
3.
Objective
The purpose of this study was to analyze the clinical features and prognostic factors of surgical outcome of foot drop caused by lumbar degenerative disease and put forward the clinical stage.Methods
We retrospectively reviewed 135 patients with foot drop due to lumbar degenerative disease. The clinical features and mechanism were analyzed. Age, sex, duration of palsy, preoperative muscle strength of tibialis anterior (TA), sensation defect of affected lower limb, affected foot, diagnosis and compressed nerve roots were recorded and compared with surgical outcome.Results
Foot drop was observed in 8.1% of all inpatients of lumbar degenerative disease. L5 nerve root compression was observed in 126 of all 135 patients (93.3%). Single, double and triple roots compression was observed respectively in 43, 83, and 9 patients (31.9%, 61.5%, and 6.6%). But there was no significant relationship between preoperative muscle strength of TA and the number of compressed roots. The muscle strength of TA was improved in 113 (83.7%) patients after surgery, but it reached to >=4 in only 21 (15.6%) patients. Improvement of the muscle strength of TA was almost stable at the 6-month follow-up. At the last follow-up, the muscle strength of TA was 1, 2, 3, 4, 5 respectively in 28, 24, 62, 13, 8 patients. Multivariate logistic regression showed duration of palsy (p=0.0360, OR=2.543), preoperative muscle strength of TA (p=0.0064, OR=5.528) and age (p=0.0309, OR=3.208) were factors that influenced recovery following an operation.Conclusions
L5 nerve root was most frequently affected. The muscle strength of TA improved in most patients after surgery, but few patients can get a good recovery from foot drop. Patients of shorter duration of palsy, better preoperative muscle strength of TA and younger age showed a better surgical outcome. 相似文献4.
Xiaopeng An Long Bai Jinxing Hou Haibo Zhao Jiayin Peng Yunxuan Song Jiangang Wang Binyun Cao 《Molecular biology reports》2013,40(2):857-863
In this study, we cloned the full coding region of NGF gene from the caprine ovary. Result showed the caprine NGF cDNA (GenBank Accession No. JQ308184) contained a 726 bp open reading frame encoding a protein with 241 amino acid residues. Bioinformatic analysis indicated that caprine NGF amino acid sequence was 83–99 % identical to that of mouse, pig, dog, human and bovine. It was predicted that caprine NGF contained nine serine phosphorylation loci, four threonine phosphorylation loci and nine specific PKC phosphorylation loci. The NGF mRNA expression pattern showed that NGF gene was expressed highly in ovary. This work provided an important experimental basis for further research on the function of NGF in goat. A single nucleotide polymorphism (A705G) in the coding region of NGF gene was detected by PCR–RFLP and DNA sequencing in 630 goats of three breeds. The frequencies of G allele were 0.52–0.61, and frequencies of A allele were 0.48–0.39 for SN, GZ and BG breeds, respectively. The does with GG genotype had higher litter size than those with GA and AA genotypes (P < 0.05). Hence, the biochemical and physiological functions, together with the results obtained in our investigation, suggest that the NGF gene could serve as a genetic marker for litter size in goat breeding. 相似文献
5.
6.
7.
Jiangang Yang Chaoyu Tian Tong Zhang Chenxi Ren Yueming Zhu Yan Zeng Yan Men Yuanxia Sun Yanhe Ma 《Biotechnology and bioengineering》2019,116(4):745-756
D -Allulose 3-epimerase (DAE) has been applied to produce D -allulose, a low-calorie and functional sweetener. In this study, a new DAE from Paenibacillus senegalensis was characterized in Escherichia coli. Furthermore, we presented a tandem isoenzyme gene expression strategy to express multiple DAEs in one cell and construct food-grade expression systems based on Corynebacterium glutamicum. Seventeen expression cassettes based on three DAE genes from different organisms were constructed. Among all recombinant strains, DAE16 harboring three DAE genes in an expression vector exhibited the highest enzyme activity with 22.7 U/mg. Whole-cell transformation of DAE16 produced 225 g/L D -allulose with a volumetric productivity of 353 g·g −1·hr −1. The catalytic efficiency of strain C-DAE9 integrating total 11 DAE genes in chromosome was 16.4-fold higher than strains carrying one DAE. Fed-batch culture of C-DAE9 gave enzyme activity of 44,700 U/L. We also expressed a thermostable invertase in C. glutamicum and obtained enzyme activity of 29 U/mg. Immobilized cells expressing DAE or invertase exhibited 80% of retained activity after 30 cycles of catalytic reactions. Those immobilized cells were coupled to produce 61.2 g/L D -allulose from cane molasses in a two-step reaction process. This study provided an efficient approach for enzyme preparation and allowed access to produce D -allulose from other abundant and low-cost feedstock enriched with sucrose. 相似文献
8.
长喙田菁-Azorhizobium caulinodans共生固氮体系在华南地区的生长、结瘤、固氮和种子生产 总被引:5,自引:0,他引:5
研究了长喙田菁-Azorhizobiumcaulinodans共生固氮体系在华南地区的生长、结瘤、固氮和种子生产.结果表明,长喙田菁-A.caulinodans共生固氮体系在华南地区生长正常,并具旺盛的茎根瘤结瘤作用.经65天的生长,其在湛江地区的单位面积生物量(干物质)和单位面积N产量分别为28752和681kg·hm-2,远优于普通田菁的16520和352kg·hm-2;茎瘤菌A.caulinodans品系AR111和AR56在华南地区混合接种效果良好,植株茎瘤结瘤率达到100%,平均单株茎瘤个数为182个,单株茎瘤鲜重约为1.2g,茎瘤生物量在茎根瘤总生物量中所占比重为70%,而其根瘤生物量略高于普通田菁;长喙田菁在华南地区能够正常开花结实,在栽植密度为4×104株·hm-2的条件下,其种子产量达3200kg·hm-2. 相似文献
9.
After the successful completion of the human genome project (HGP), biological research in the postgenome era urgently needs an efficient approach for functional analysis of genes. Utilization of knockout mouse models has been powerful for elucidating the function of genes as well as finding new therapeutic interventions for human diseases. Gene trapping and gene targeting are two independent techniques for making knockout mice from embryonic stem (ES) cells. Gene trapping is high‐throughput, random, and sequence‐tagged while gene targeting enables the knockout of specific genes. It has been about 20 years since the first gene targeting and gene trapping mice were generated. In recent years, new tools have emerged for both gene targeting and gene trapping, and organizations have been formed to knock out genes in the mouse genome using either of the two methods. The knockout mouse project (KOMP) and the international gene trap consortium (IGTC) were initiated to create convenient resources for scientific research worldwide and knock out all the mouse genes. Organizers of KOMP regard it as important as the HGP. Gene targeting methods have changed from conventional gene targeting to high‐throughput conditional gene targeting. The combined advantages of trapping and targeting elements are improving the gene trapping spectrum and gene targeting efficiency. As a newly‐developed insertional mutation system, transposons have some advantages over retrovirus in trapping genes. Emergence of the international knockout mouse consortium (IKMP) is the beginning of a global collaboration to systematically knock out all the genes in the mouse genome for functional genomic research. genesis 48:73–85, 2010. © 2010 Wiley‐Liss, Inc. 相似文献
10.