全文获取类型
收费全文 | 260篇 |
免费 | 22篇 |
出版年
2021年 | 3篇 |
2020年 | 4篇 |
2019年 | 1篇 |
2018年 | 2篇 |
2017年 | 2篇 |
2016年 | 8篇 |
2015年 | 16篇 |
2014年 | 15篇 |
2013年 | 23篇 |
2012年 | 16篇 |
2011年 | 15篇 |
2010年 | 7篇 |
2009年 | 10篇 |
2008年 | 13篇 |
2007年 | 14篇 |
2006年 | 14篇 |
2005年 | 10篇 |
2004年 | 16篇 |
2003年 | 16篇 |
2002年 | 15篇 |
2001年 | 7篇 |
2000年 | 3篇 |
1999年 | 3篇 |
1998年 | 5篇 |
1997年 | 3篇 |
1996年 | 7篇 |
1995年 | 5篇 |
1994年 | 3篇 |
1993年 | 3篇 |
1992年 | 2篇 |
1991年 | 2篇 |
1990年 | 1篇 |
1989年 | 4篇 |
1987年 | 3篇 |
1986年 | 2篇 |
1985年 | 1篇 |
1984年 | 1篇 |
1983年 | 3篇 |
1980年 | 2篇 |
1976年 | 1篇 |
1974年 | 1篇 |
排序方式: 共有282条查询结果,搜索用时 296 毫秒
1.
Chris J. van Koppen Marcel W. Hermanussen Kiek N. Verrijp Jaap F. Rodrigues de Miranda Arie J. Beld Jan-Willem J. Lammers Cees A. M. van Ginneken 《Life sciences》1987,40(26):2561-2570
Specific binding of [125I]-(−)-cyanopindolol to human tracheal smooth muscle membranes was saturable, stereo-selective and of high affinity (Kd=5.3±0.9 pmol/l and RT=78±7fmol/g tissue). The β1-selective antagonists atenolol and LK 203-030 inhibited specific [125I]-(−)-cyanopindolol binding according to a one binding site model with low affinity in nearly all subjects, pointing to a homogeneous β2-adrenoceptor population. In one subject using LK 203-030 a small β-adrenoceptor subpopulation could be demonstrated. The beta-mimetics isoprenaline, fenoterol, salbutamol and terbutaline recognized high and low affinity agonist binding sites. Isoprenaline's pKH- and pKL- values for the high and low affinity sites were 8.0±0.2 and 5.9±0.3 respectively. In functional experiments isoprenaline relaxed tracheal smooth muscle strips having intrinsic tone with a pD2-value of 6.63±0.19. 相似文献
2.
Fourteen commercial leek fields with first and second generation Leek moth, Acrolepiopsis assectella Z., (LM) injury were sampled during 1986 in The Netherlands. For both generations, plant injury was more prevalent in the perimeter of the field than in interior portions. A sequential sampling program for use in making treatment decisions for LM was developed. An economic analysis of the value of sampling information derived from the sequential sampling program was performed. The parameters used for the analysis were crop yield and value, expected level of LM infestation, potential loss of value due to LM infestation, effectiveness of insecticide application, and cost of sampling. Due mainly to the high value of the crop and low cost of treatment, analysis indicates that there is little difference between a sampling-based management plan and prophylactic application of insecticides in terms of pest control costs. Additionally, such a sampling-based management plan is relatively insensitive to changes in the parameters used in the model. Thus, development of a threshold linked to a sampling procedure will not reduce pest control costs. Use of the sampling-based management plan will also not significantly increase pest control costs and will likely result in reduced insecticide use compared with a prophylactic treatment program.
Zusammenfassung In den Niederlanden wurde 1986 der durch den Befall der ersten und zweiten Generation der Lauchmotte (Acrolepiosis assectella Z.) verursachte Schaden auf 14 kommerziell genutzten Porreefeldern ermittelt. Für beide Generationen wurde an den Feldrändern ein deutlich höherer Befall als in dem inneren Bereich eines Feldes festgestellt. Zur Bekämpfung der Lauchmotte wurde als Entscheidungsmodell ein sequentielles Probenahmeverfahren entwickelt. Der Informationsgehalt der sequentiellen Befallserhebung wurde einer ökonomischen Bewertung unterzogen. Hierbei wurden folgende Parameter zugrunde gelegt: Erntemenge und Ertrag, erwarteter Befallsgrad sowie hierdurch verursachter möglicher Ertragsverlust, Wirkungsgrad einer Insektizidbehandlung und Kosten der Stichprobennahme. Die Analyse der einzelnen Parameter ergab nur eine geringe Kostendifferenz zwischen einer gezielten Bekämpfung nach sequentieller Probenahme und prophylaktischen Insektizidspritzungen, bedingt durch den hohen Wert der Kultur und die niedrigen Kosten einer Bekämpfung. Darüber hinaus its das Verfahren einer gezielten Lauchmottenbekämpfung gegeüber Veränderungen der obengenannten Parameter relativ unempfindlich. Deshalb wird die Entwicklung eines Schwellenwertes der mit Probenahmeverfahren verbunden ist die Bekämpfungskosten nicht reduzieren. Die anwendung der Probenahmeplans würde Bekämpfungskosten auch nicht erhöhen und würde wahrscheinlich Insecktizidverbrauch reduzieren im Vergleich zu einen prophylaktischen Behandlungsprogramm.相似文献
3.
4.
Jan-Willem Taanman Anneke Y. van der Veen Cobi Schrage Hans de Vries Charles H. C. M. Buys 《Human genetics》1991,87(3):325-327
Summary A cloned, 40 kb, genomic DNA fragment, containing the last exon of the gene for human cytochrome c oxidase subunit VIb and its flanking sequences, was used as a probe to localize the subunit VIb gene on human metaphase chromosomes. The probe was labelled with Bio-11-dUTP and detected by fluorescence. Subsequent R-banding indicated that the cytochrome c oxidase subunit VIb gene is localized in band 19q13.1, extending the evidence that the human nuclear genes of cytochrome c oxidase are not clustered. 相似文献
5.
6.
A total of 24 commercial fields of cabbages and Brussels sprouts were sampled in a grid fashion with 20–25 equally spaced
cells with four plants per cell. Using this data base of 80–100 plants, we conducted computer stimulations to compare the
treatment decisions that would be made for the major insect pests using published sequential sampling programs and a newly
developed variable-intensity sampling program. Additionally, we compared the number of samples required to make the decision.
At low thresholds (10–20%) for both Lepidoptera and cabbage aphids, variable intensity-sampling required a smaller sample
size and provided more reliable decisions, while at high thresholds (40–50%) sequential sampling provided more reliable decisions.
In both procedures, the occurrence of incorrect decisions was minimal. The number of cases in which a decision would not be
reached after a 40-plant sample was lower for variable-intensity sampling. Considering the number of samples required to make
a correct decision and the greater need for reliable decisions at lower thresholds, variable-intensity sampling was superior
to sequential sampling. Additionally, variable-intensity sampling has the advantage of requiring samples to be taken in a
greater area of the field and thus increases the probability of detecting localized infestations. Although variable-intensity
sampling was not designed to classify pest populations for treatment decisions but rather to achieve sampling precision around
the population mean, our present studies indicate that it can also be an effective method to aid in treatment decisions. 相似文献
7.
Regulation of oxidative phosphorylation: The flexible respiratory network ofParacoccus denitrificans
8.
Hendrica A. Akster Jan-Willem van de Wal Tineke Veenendaal 《Cell and tissue research》1995,281(3):517-524
At muscle-tendon junctions of red and of white axial muscle fibres of carp, new sarcomeres are found adjacent to existing sarcomeres along the bundles of actin filaments that connect the myofibrils with the junctional sarcolemma. As the filament bundles that transmit force to the junction originate proximal to new sarcomeres, they probably relieve these new sarcomeres from premature loading. In red fibres, these filament bundles are long (up to 20 m) and dense, permitting light-microscopical immunohistochemistry (double reactions: anti-titin or anti--actinin and phalloidin). New sarcomeres have clear I bands; their A band lengths are similar to those of older sarcomeres and the thick filaments lie in register. T tubules are found at the distal side of new sarcomeres but terminal Z lines are absent. The late addition of -actinin suggests that -actinin mainly has a stabilizing role in sarcomere formation. The presence of titin in the terminal fibre protrusions is in agreement with its supposed role in sarcomere formation, viz. the integration of thin and thick filaments. The absence of a terminal Z line from sarcomeres with well-registered A bands suggests that this structure is not essential for the anchorage of connective (titin) filaments. 相似文献
9.
Intercropping as a method to reduce crop losses is an old technique, especially in the tropics. During 1977 and 1978 intercropping experiments of Brussels sprouts with Spergula arvensis showed a significant pest reducing effect. Mamestra brassicae and Evergestis forficalis populations showed a strong reduction, whereas Pieris rapae did not seem to respond to intercropping. Late cabbage root fly infestation of the sprout buttons was reduced drastically. Brevicoryne brassicae incidence was decreased in dense intercropping plots only. Injury due to caterpillar feeding showed a grading response to intercropping density, as did the total caterpillar populations. Log dose-effect regression lines allow a comparison of various intercropping densities with other control methods on insect pest population reducing potential.
Zusammenfassung Durch Untersaaten können Ertragsverluste an Kulturpflanzen reduziert werden. Das ist ein altes, bewährtes Verfahren, besonders in den Tropen. Die Einsaat von Spergula arvensis in Rosenkohlbeständen führte in 2-jährigen Untersuchungen zur signifikanten Verringerung des Schädlingsbefalls. Während die Populationen von Mamestra brassicae und Evergestis forficalis stark zurückgingen, blieb die von Pieris rapae unbeeinflusst. Eine Abnahme von Brevicoryne brassicae konnte nur in Parzellen mit hohen Spergula-Dichten festgestellt werden. Die Abundanz der Raupen sowie die Beschädigungsintensität durch diese, zeigten eine Abhängigkeit von der Untersaatdichte. Die Regressionsgerade Untersaatdichte zur Effektivität erlaubt einen Vergleich verschiedener Spergula-Dichten mit anderen Methoden, die das Schädlingspotential reduzieren.相似文献
10.
RNA and DNA binding zinc fingers in Xenopus TFIIIA. 总被引:4,自引:0,他引:4