全文获取类型
收费全文 | 1424篇 |
免费 | 94篇 |
出版年
2023年 | 11篇 |
2022年 | 10篇 |
2021年 | 31篇 |
2020年 | 19篇 |
2019年 | 26篇 |
2018年 | 38篇 |
2017年 | 32篇 |
2016年 | 45篇 |
2015年 | 64篇 |
2014年 | 65篇 |
2013年 | 126篇 |
2012年 | 111篇 |
2011年 | 97篇 |
2010年 | 69篇 |
2009年 | 58篇 |
2008年 | 91篇 |
2007年 | 65篇 |
2006年 | 66篇 |
2005年 | 65篇 |
2004年 | 54篇 |
2003年 | 63篇 |
2002年 | 48篇 |
2001年 | 18篇 |
2000年 | 12篇 |
1999年 | 20篇 |
1998年 | 13篇 |
1997年 | 7篇 |
1996年 | 12篇 |
1995年 | 12篇 |
1994年 | 8篇 |
1993年 | 12篇 |
1992年 | 8篇 |
1991年 | 13篇 |
1990年 | 11篇 |
1989年 | 9篇 |
1988年 | 8篇 |
1987年 | 11篇 |
1986年 | 6篇 |
1985年 | 8篇 |
1984年 | 8篇 |
1983年 | 8篇 |
1981年 | 4篇 |
1980年 | 5篇 |
1979年 | 4篇 |
1978年 | 4篇 |
1977年 | 4篇 |
1975年 | 3篇 |
1968年 | 5篇 |
1967年 | 3篇 |
1960年 | 2篇 |
排序方式: 共有1518条查询结果,搜索用时 125 毫秒
1.
Benjamin C. Blum Weiwei Lin Matthew L. Lawton Qian Liu Julian Kwan Isabella Turcinovic Ryan Hekman Pingzhao Hu Andrew Emili 《Molecular & cellular proteomics : MCP》2022,21(1):100189
Metabolism is recognized as an important driver of cancer progression and other complex diseases, but global metabolite profiling remains a challenge. Protein expression profiling is often a poor proxy since existing pathway enrichment models provide an incomplete mapping between the proteome and metabolism. To overcome these gaps, we introduce multiomic metabolic enrichment network analysis (MOMENTA), an integrative multiomic data analysis framework for more accurately deducing metabolic pathway changes from proteomics data alone in a gene set analysis context by leveraging protein interaction networks to extend annotated metabolic models. We apply MOMENTA to proteomic data from diverse cancer cell lines and human tumors to demonstrate its utility at revealing variation in metabolic pathway activity across cancer types, which we verify using independent metabolomics measurements. The novel metabolic networks we uncover in breast cancer and other tumors are linked to clinical outcomes, underscoring the pathophysiological relevance of the findings. 相似文献
2.
The potential for reducing the occurrence of shallow landslides through targeted reforestation of critical parts of a river basin is explored through mathematical modelling. Through the systematic investigation of land management options, modelling allows the optimum strategies to be selected ahead of any real intervention in the basin. Physically based models, for which the parameters can be evaluated using physical reasoning, offer particular advantages for predicting the effects of possible future changes in land use and climate. Typically a physically based landslide model consists of a coupled hydrological model (for soil moisture) and a geotechnical slope stability model, along with an impact model, such as basin sediment yield. An application of the SHETRAN model to the 65.8-km2 Guabalcón basin in central Ecuador demonstrates a technique for identifying the areas of a basin most susceptible to shallow landsliding and for quantifying the effects of different vegetation covers on landslide incidence. Thus, for the modelled scenario, increasing root cohesion from 300 to 1500 Pa causes a two-thirds reduction in the number of landslides. Useful information can be obtained even on the basis of imperfect data availability but model output should be interpreted carefully in the light of parameter uncertainty. 相似文献
3.
4.
Tetyana Denysenko Luisa Gennero Carola Juenemann Isabella Morra Paolo Masperi Vincenzo Ceroni Antonella Pragliola Antonio Ponzetto Antonio Melcarne 《Cell biochemistry and function》2014,32(2):164-176
Glioblastomas (GBMs) are the most lethal primary brain tumours. Increasing evidence shows that brain tumours contain the population of stem cells, so‐called cancer stem cells (CSCs). Stem cell marker CD133 was reported to identify CSC population in GBM. Further studies have indicated that CD133 negative cells exhibiting similar properties and are able to initiate the tumour, self‐renew and undergo multilineage differentiation. GBM is a highly heterogeneous tumour and may contain different stem cell populations with different functional properties. We characterized five GBM cell lines, established from surgical samples, according to the marker expression, proliferation and differentiation potential. CD133 positive cell lines showed increased proliferation rate in neurosphere condition and marked differentiation potential towards neuronal lineages. Whereas two cell lines low‐expressing CD133 marker showed mesenchymal properties in vitro, that is high proliferation rate in serum condition and differentiation in mesenchymal cell types. Further, we compared therapy resistance capacity of GBM cell lines treated with hydroxyurea. Our results suggest that CSC concept is more complex than it was believed before, and CD133 could not define entire stem cell population within GBM. At least two different subtypes of GBM CSCs exist, which may have different biological characteristics and imply different therapeutic strategies. Copyright © 2013 John Wiley & Sons, Ltd. 相似文献
5.
Bianca Colonna Maria Bernardini Gioacchino Micheli Francesco Maimone Mauro Nicoletti Mariassunta Casalino 《Plasmid》1988,20(3):221-231
Tn1935, a 23.5-kb transposon mediating resistance to ampicillin, kanamycin, mercury, spectinomycin, and sulfonamide was isolated from pZM3, an IncFIme virulence plasmid from Salmonella wien. Tn1935 possesses the entire sequence of Tn21 and contains two additional DNA segments of 0.95 and 2.7 kb carrying the ampicillin and kanamycin resistance genes, respectively. The latter is part of a composite element since it is flanked by two IS15-like insertion sequences (IS1936) in direct orientation. IS1936 is about 800 bp long and is closely related to IS15 delta, IS26, IS46, IS140, and IS176. Functional analysis of IS1936-mediated cointegrates shows that both insertion sequences are active and able to form cointegrates at the same frequency. Resolution of the cointegrates requires the presence of the host Rec system. The presence of the composite IS1936-element within Tn1935 supports the hypothesis that multidrug resistance transposons evolved by insertion of antibiotic determinants which are themselves transposable. 相似文献
6.
Bruna Tedeschi Patrizia Vernole M.Lucia Sanna Benedetto Nicoletti 《Human genetics》1992,89(5):543-547
Summary Chromosome fragile sites are inducible by aphidicolin in cultured human lymphocytes. To assess the frequency and distribution of these common fragile sites in the general population, a cytogenetic survey was performed on 126 subjects, 59 males and 67 females, whose age ranged from 1 day to 72 years. Common fragile sites, induced by aphidicolin, were widespread and showed a remarkably different sensitivity among individuals; age influenced the overall frequency of fragile sites. Moreover, both age and sex seemed to modulate the expression of specific fragile sites. In our population, the most common fragile sites were: 3p14, 16q23, Xp22, 6q26, 1p31, 4q31, 1p22, 7q22, 2q33, 3q27, 2q31, 7q32, 14q24, 10q22, 5q31, 2q37, 6p21. 相似文献
7.
8.
DQ α and β RFLP reveals the composition of the DQ molecule recognized by T-cell clones 总被引:2,自引:0,他引:2
Sharon Rosenshine Isabella Cascino Adriana Zeevi René J. Duquesnoy Massimo Trucco 《Immunogenetics》1986,23(3):187-196
Pst I RFLP, revealed with DQ
and DQ
probes, was compared with Taq I RFLP using a panel of DR-homozygous cell lines and HLA-typed family members. Taq I patterns, characteristic for each DR-associated DQ and allelic forms, were recognized in the homozygous state and then proven to segregate in the heterozygous members of informative families. The presence of both specific and chains was found to be necessary to form the type of DQ molecule specifically recognized by two alloreactive T-cell clones. Particular and associations also seem to be responsible for some Dw splits of the DRw6-positive cells. Taq I RFLP analysis may be more complex than the Pst I analysis, but is certainly more informative and complete, considering the type of information we were seeking by performing these types of experiments.Abbreviations used in this paper BSA
bovine serum albumin
- GLO
glyoxalase
- kb
kilobase(s)
- LCL
lymphoblastoid cell line
- MHC
major histocompatibility complex
- PBL
peripheral blood lymphocyte
- PLT
primed lymphocyte test
- RFLP
restriction fragment length polymorphism
- SDS
sodium dodecyl sulfate
- SSC
standard sodium citrate
- SSCP
sodium, sodium citrate, sodium phosphate
- TBE
Tris-borate, boric acid, ethylenediaminetetraacetate (EDTA)
- TCGF
T-cell growth factor 相似文献
9.
C. Millia F. Nicoletti A. A. Grasso F. Patti D. F. Condorelli E. Rapisarda† L. Rampello G. Costa† U. Scapagnini 《Journal of neurochemistry》1983,41(4):1190-1191
Abstract: Previous data indicate that the injection of dopaminergic drugs induces changes in cerebellar 3',5'-guanosine monophosphate (cGMP) content. Accordingly, we have investigated the effects of haloperidol, sulpiride, or apomorphine on cerebellar prostaglandin (PG) concentration, a parameter related to cGMP content. Results obtained show that dopamine receptor blocking agents, such as haloperidol and sulpiride, significantly decrease cerebellar PGE2 and PGF2α concentrations, while opposite changes are induced by apomorphine, a dopamine receptor agonist. 相似文献
10.
G. Clementi F. Nicoletti F. Patacchioli A. Prato F. Patti C.E. Fiore † M. Matera U. Scapagnini 《Journal of neurochemistry》1983,40(3):885-886
Abstract: The effects of calcitonin on neurochemical parameters related to the tuberoinfundibular dopaminergic system have been investigated in an attempt to elucidate how calcitonin decreases serum prolactin levels. Intracerebroventricular human or salmon calcitonin injection decreases serum prolactin, medial basal hypothalamic dopamine (DA) and dihydroxyphenylacetic acid (DOPAC) and hypophysial DA and increases hypophysial DOPAC. Results suggest that calcitonin may decrease prolactin secretion via the tuberoinfundibular dopaminergic system. 相似文献