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1.
T S Il'ina 《Genetika》1986,22(11):2572-2582
The published data on molecular mechanisms of transposons movement inside and between genomes are reviewed. The replicative mechanism of transposition of the family of Tn3-like elements is discussed, as well as the modes of bacteriophage Mu, Tn9, Tn10, Tn903 transposition. The factors affecting the choice of transposition pathways are analysed. 相似文献
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V P Torchilin N M Mukhamedova A I Il'ina L Z Iakubov K G Shchitkov 《Biulleten' eksperimental'no? biologii i meditsiny》1992,114(10):400-401
The study has demonstrated the possibility to use cyclophosphamide as a model of "impairment" agent for lung tissue cells to provide access of monoclonal antibodies to nuclear antigen structures. Biodistribution of monoclonal antibodies to various intracellular antigens was studied on Wistar rats pretreated with various doses of cyclophosphamide. Accumulation of 2C5 antibodies to cell nuclei was found to be dependent on the dose of cyclophosphamide administered. 相似文献
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I S Sever T S Il'ina V L Motin G B Smirnov 《Molekuliarnaia genetika, mikrobiologiia i virusologiia》1991,(12):10-14
The phenotypic properties conferred to Yersinia pseudotuberculosis cells by the genetical determinants of a 25Md fragment of the plasmid pVM82 coding for the modified cellular immune response in the infected organism. The fragment was shown to determine the conjugative properties of the plasmid, the resistance of bacterial cells to a number of hydrophobic agents and cellular ability to absorb the Congo red dye. The latter confirms the presence of additional structural components in the cell wall of the strain harbouring the plasmid pVM82. The increased resistance of the plasmid-containing strain to bactericidal effect of the blood plasma was demonstrated as compared with the resistance of the strains harbouring the p57 plasmid lacking the 25Md fragment or no plasmid at all. 相似文献
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Fushitani K; Higashiyama K; Moriyama EN; Imai K; Hosokawa K 《Molecular biology and evolution》1996,13(7):1039-1043
To elucidate phylogenetic relationships among amniotes and the evolution of
alpha globins, hemoglobins were analyzed from the Komodo dragon (Komodo
monitor lizard) Varanus komodoensis, the world's largest extant lizard,
inhabiting Komodo Islands, Indonesia. Four unique globin chains (alpha A,
alpha D, beta B, and beta C) were isolated in an equal molar ratio by high
performance liquid chromatography from the hemolysate. The amino acid
sequences of two alpha chains were determined. The alpha D chain has a
glutamine at E7 as does an alpha chain of a snake, Liophis miliaris, but
the alpha A chain has a histidine at E7 like the majority of hemoglobins.
Phylogenetic analyses of 19 globins including two alpha chains of Komodo
dragon and ones from representative amniotes showed the following results:
(1) The a chains of squamates (snakes and lizards), which have a glutamine
at E7, are clustered with the embryonic alpha globin family, which
typically includes the alpha D chain from birds; (2) birds form a sister
group with other reptiles but not with mammals; (3) the genes for embryonic
and adult types of alpha globins were possibly produced by duplication of
the ancestral alpha gene before ancestral amniotes diverged, indicating
that each of the present amniotes might carry descendants of the two types
of alpha globin genes; (4) squamates first split off from the ancestor of
other reptiles and birds.
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Domnikova NP Briakotnina EV Il'ina VN Evstropov AN 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》2004,(3):3-6
A total of 67 patients with blood system diseases and infectious complications were examined. During the period of the examination 139 microorganisms were isolated. Of these gram negative microorganisms constituted 51%, gram positive microorganisms--34.8% and fungal flora--14.2%. Most frequently the following gram negative microorganisms were isolated from the patients: Pseudomonas sp. (including P. aeruginosa), Klebsiella pneumoniae, Escherichia coli, Haemophilus influenzae. All isolated microorganisms retained sensitivity to imipenem, with the exception of individual strains of Pseudomonas sp.; the latter exhibited sensitivity to amicacin and ceftazidim. Cefotaxime was active with respect to 75% of K. pneumoniae strains and all E. coli strains, ciprofloxacin was active with respect to 43% of E. coli strains, 80% of K. pneumoniae strains and 83.4% of Pseudomonas sp. strains, cefepim was active with respect to 85.7% of Pseudomonas sp. strains and all E. coli strains, ceftazidim was active with respect to all Pseudomonas sp. and E. coli strains. 75% of K. pneumoniae strains, 77.8% of Pseudomonas sp. strains and 86% of E. coli strains retained sensitivity to amicacin. 25% of K. pneumoniae strains required testing for ESBL production. 相似文献
10.
The possibility for forming physical gels based on Pchelozan (bee chitosan with a molecular weight of 230 kDa and an acetylation degree of 26-65%) has been demonstrated. Conditions for obtaining the gels (1% solution in 1% glycolic acid, 25 degrees C, pH 5.5-7.5) were selected. The effects of (1) the concentration of the original solution, (2) the degree of acetylation of Pchelozan, and (3) the value of pH on the process of gel formation, were studied. The gels obtained may be classified with reversible toxotropic systems. These gels are stable for a long time when stored within a temperature range of 18-55 degrees C. The gel with a degree of acetylation of 34% was characterized by irreversible syneresis. 相似文献