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排序方式: 共有1054条查询结果,搜索用时 31 毫秒
1.
Elrashdy M. Redwan Vladimir N. Uversky Esmail M. El-Fakharany Hussein Al-Mehdar 《Comptes rendus biologies》2014,337(10):581-595
Lactoferrin (LF) is an 80-kDa globular glycoprotein with high affinity for metal ions, particularly for iron. This protein possesses many biological functions, including the binding and release of iron and serves as one of the important components of the innate immune system, where it acts as a potent inhibitor of several pathogens. LF has efficacious antibacterial and antiviral activities against a wide range of Gram-positive and Gram-negative bacteria and against both naked and enveloped DNA and RNA viruses. In its antiviral pursuit, LF acts predominantly at the acute phase of the viral infection or even at the intracellular stage, as in hepatitis C virus infection. LF inhibits the entry of viral particles into host cells, either by direct attachment to the viral particles or by blocking their cellular receptors. This wide range of activities may be attributed to the capacity of LF to bind iron and its ability to interfere with the cellular receptors of both hosts and pathogenic microbes. 相似文献
2.
A. A. Moaty Sayed M. A. Hussein T. Becker 《Biomechanics and modeling in mechanobiology》2010,9(2):141-151
Lattice Boltzmann models (LBM) are rapidly showing their ability to simulate a lot of fluid dynamics problems that previously
required very complex approaches. This study presents a LBM for simulating diffusion–advection transport of substrate in a
2-D laminar flow. The model considers the substrate influx into a set of active cells placed inside the flow field. A new
innovative method was used to simulate the cells activity using the LBM by means of Michaelis–Menten kinetics. The model is
validated with some numerical benchmark problems and proved highly accurate results. After validation the model was used to
simulate the transport of oxygen substrates that diffuse in water to feed a set of active cartilage cells inside a new designed
bioreactor. 相似文献
3.
Nazar J. Hussein Thomas Mbimba Asaad A. Al-Adlaan Mohammad Y. Ansari Fatima A. Jaber Scott McDermott Takhar Kasumov Fayez F. Safadi 《Journal of cellular biochemistry》2020,121(1):284-298
Trafficking protein particle complex 9 (TRAPPC9) is a major subunit of the TRAPPII complex. TRAPPC9 has been reported to bind nuclear factor κB kinase subunit β (IKKβ) and NF-kB-inducing kinase (NIK) where it plays a role in the canonical and noncanonical of nuclear factor-κB (NF-kB) signaling pathways, receptively. The role of TRAPPC9 in protein trafficking and cytoskeleton organization in osteoclast (OC) has not been studied yet. In this study, we examined the mRNA expression of TRAPPC9 during OC differentiation. Next, we examined the colocalization of TRAPPC9 with cathepsin-K, known to mediate OC resorption suggesting that TRAPPC9 mediates the trafficking pathway within OC. To identify TRAPPC9 protein partners important for OC-mediated cytoskeleton re-organization, we conducted immunoprecipitation of TRAPPC9 in mature OCs followed by mass spectrometry analysis. Our data showed that TRAPPC9 binds various protein partners. One protein with high recovery rate is L-plastin (LPL). LPL localizes at the podosomes and reported to play a crucial role in actin aggregation thereby actin ring formation and OC function. Although the role of LPL in OC-mediated bone resorption has not fully reported in detail. Here, first, we confirmed the binding of LPL to TRAPPC9 and, then, we investigated the potential regulatory role of TRAPPC9 in LPL-mediated OC cytoskeleton reorganization. We assessed the localization of TRAPPC9 and LPL in OC and found that TRAPPC9 is colocalized with LPL at the periphery of OC. Next, we determined the effect of TRAPPC9 overexpression on LPL recruitment to the actin ring using a viral system. Interestingly, our data showed that TRAPPC9 overexpression promotes the recruitment of LPL to the actin ring when compared with control cultures. In addition, we observed that TRAPPC9 overexpression reorganizes actin clusters/aggregates and regulates vinculin recruitment into the OC periphery to initiate podosome formation. 相似文献
4.
Shadia E. Ramadan A. A. Razak A. M. Ragab M. El-Meleigy 《Biological trace element research》1989,20(3):225-232
Aspergillus fumigatus, Aspergillus terreus, and Penicillium chrysogenum, a tellurium tolerant fungi, are able to grow on sulfur free medium amended with 0.2% (w/v) tellurite. Tellurium was incorporated into several types of low and high molecular weight proteins. The newly detected telluro-proteins contained an extraordinary high level of tellurium, as well as telluro-cysteine, telluro-cystine, telluro-methionine, and serine. 相似文献
5.
Suitability of different mite prey for the development of the predatory mite,Phytoseiulus persimilis
Development of the phytoseiid mitePhytoseiulus persimilis Athias-Henriot was studied when fed on three mite species as prey. The tenuipalpid mite,Brevipalpus pulcher (Canestrini & Fanzago), is an unsuitable prey forP. persimilis as predatory larvae reared on any stages developed to the protonymphal stage only. WhenP. persimilis larvae were reared on the eriophyid mite,Eriophyes dioscoridis Soliman & Abou-Awad, only a few developed to adulthood, but failed to oviposit. On the contrary, predatory larvae fed on the tetranychid mite,Tetranychus urticae Koch, matured in a significantly shorter period and resulting females exhibited a high rate of reproduction. 相似文献
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A study was conducted to determine the role of inoculum size of a bacterium introduced into nonsterile lake water in the biodegradation of a synthetic chemical. The test species was a strain of Pseudomonas cepacia able to grow on and mineralize 10 ng to 30 micrograms of p-nitrophenol (PNP) per ml in salts solution. When introduced into water from Beebe Lake at densities of 330 cells per ml, P. cepacia did not mineralize 1.0 microgram of PNP per ml. However, PNP was mineralized in lake water inoculated with 3.3 X 10(4) to 3.6 X 10(5) P. cepacia cells per ml. In lake water containing 1.0 microgram of PNP per ml, a P. cepacia population of 230 or 120 cells per ml declined until no cells were detectable at 13 h, but when the initial density was 4.3 X 10(4) cells per ml, sufficient survivors remained after the initial decline to multiply at the expense of PNP. The decline in bacterial abundance coincided with multiplication of protozoa. Cycloheximide and nystatin killed the protozoa and allowed the bacterium to multiply and mineralize 1.0 microgram of PNP, even when the initial P. cepacia density was 230 or 360 cells per ml. The lake water contained few lytic bacteria. The addition of KH2PO4 or NH4NO3 permitted biodegradation of PNP at low cell densities of P. cepacia. We suggest that a species able to degrade a synthetic chemical in culture may fail to bring about the same transformation in natural waters, because small populations added as inocula may be eliminated by protozoan grazing or may fail to survive because of nutrient deficiencies. 相似文献