全文获取类型
收费全文 | 450篇 |
免费 | 36篇 |
国内免费 | 3篇 |
出版年
2021年 | 7篇 |
2020年 | 6篇 |
2019年 | 9篇 |
2018年 | 4篇 |
2017年 | 7篇 |
2016年 | 6篇 |
2015年 | 28篇 |
2014年 | 26篇 |
2013年 | 33篇 |
2012年 | 36篇 |
2011年 | 35篇 |
2010年 | 21篇 |
2009年 | 21篇 |
2008年 | 25篇 |
2007年 | 29篇 |
2006年 | 26篇 |
2005年 | 19篇 |
2004年 | 13篇 |
2003年 | 16篇 |
2002年 | 9篇 |
2001年 | 4篇 |
2000年 | 6篇 |
1999年 | 9篇 |
1998年 | 7篇 |
1997年 | 6篇 |
1996年 | 5篇 |
1995年 | 3篇 |
1994年 | 8篇 |
1993年 | 2篇 |
1992年 | 4篇 |
1991年 | 2篇 |
1990年 | 2篇 |
1988年 | 3篇 |
1986年 | 4篇 |
1984年 | 3篇 |
1983年 | 5篇 |
1982年 | 4篇 |
1980年 | 6篇 |
1979年 | 2篇 |
1978年 | 3篇 |
1977年 | 2篇 |
1976年 | 3篇 |
1973年 | 2篇 |
1949年 | 1篇 |
1948年 | 1篇 |
1942年 | 1篇 |
1909年 | 1篇 |
1907年 | 1篇 |
1886年 | 1篇 |
1859年 | 1篇 |
排序方式: 共有489条查询结果,搜索用时 162 毫秒
1.
2.
Summary The sum of effective temperatures (SET) and lower development threshold (LDT) were established for eggs and/or pupae of central European populations of 20 species of chrysopid, coccinellid, hemerobiid, and syrphid predators of aphids. LDT ranged between 5.6° and 12.2°C, SET between 38.3 and 140.9 day degrees (dd), with broad overlap among stages and taxa. When LDT was plotted against SET, the data for both eggs and pupae were scattered along a single regression line which predicted a 0.47°C decrease in LDT per 10 dd increase in SET (r=-0.77, p<0.001). A regression calculated from published data from all over the world predicted a 0.24°C/10 dd decrease in LDT, and the data were more scattered (r=-0.38, p<0.01). This is perhaps the first report on the functional relationship between LDT and SET at the interspecific level. The species and stages differed in typical development length (VDL) and in the extent of its deceleration by low temperatures (DD). DD increased with increasing VDL, but the relative effect of low temperature on development length (DD/VDL ratio) reflected thermal adaptations consistent with the life history of the species. Polyvoltine species were less affected by low temperatures than monovoltine species, particularly the thermophilic ones. 相似文献
3.
4.
Two truncated Bacillus thuringiensis crystal protein genes, belonging to the classes cryIA(b) and cryIC and both coding for insecticidal N-terminal fragments of the corresponding crystal proteins, were translationally fused. Expression of the gene fusion in Escherichia coli showed a biologically active protein with a toxicity spectrum that overlapped those of both contributing crystal proteins. 相似文献
5.
Insect resistance of transgenic plants that express modified Bacillus thuringiensis cryIA(b) and cryIC genes: a resistance management strategy 总被引:7,自引:0,他引:7
6.
Extraction and Isolation of Antifreeze Proteins from Winter Rye (Secale cereale L.) Leaves 总被引:15,自引:3,他引:12 下载免费PDF全文
Apoplastic extracts of cold-acclimated winter rye (Secale cereale L. cv Musketeer) leaves were previously shown to exhibit antifreeze activity. The objectives of the present study were to identify and characterize individual antifreeze proteins present in the apoplastic extracts. The highest protein concentrations and antifreeze activity were obtained when the leaf apoplast was extracted with ascorbic acid and either CaCl2 or MgSO4. Seven major polypeptides were purified from these extracts by one-dimensional sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis under nonreducing conditions. The five larger polypeptides, of 19, 26, 32, 34, and 36 kD, exhibited significant levels of antifreeze activity, whereas the 11- and 13-kD polypeptides showed only weak activity. Five of these polypeptides migrated with higher apparent molecular masses on SDS gels after treatment with 0.1 M dithiothreitol, which indicated the presence of intramolecular disulfide bonds. The apparent reduction of the disulfide bonds did not eliminate antifreeze activity in four of the polypeptides that contained intramolecular disulfide bonds and exhibited significant levels of antifreeze activity. The amino acid compositions of these polypeptides were similar in that they were all relatively enriched in the residues Asp/Asn, Glu/Gln, Ser, Thr, Gly, and Ala; they all lacked His, except for the 26-kD polypeptide, and they contained up to 5% Cys residues. These polypeptides were examined with antisera to other cystine-containing antifreeze proteins from fish and insects, and no common epitopes were detected. We conclude that cold-acclimated winter rye leaves produce multiple polypeptides with antifreeze activity that appear to be distinct from antifreezes produced by fish and insects. 相似文献
7.
8.
A study was made of the changes in the content of essential oil and its components (farnesene, (-)-α-bisabolol, (-)-α-bisabololoxide
A, (-)-α-bisabololoxide B and spathulenol, α-bisabolonoxide A, chamazulene, cis en-in-dicycloether) and of the number of secretory
canals in the receptacles during the beginning of flowering, full flowering and the termination of flowering of camomile anthodia.
The total content of the essential oil was highest during the beginning of flowering and lowest during its termination. The
proportion of the individual sesquiterpenes in the essential oil underwent changes mostly during the beginning and termination
of flowering. The number of secretory canals in the basal and middle anthodial parts increased during the beginning of flowering
and the full flowering. 相似文献
9.
Antifreeze protein produced endogenously in winter rye leaves 总被引:30,自引:0,他引:30
After cold acclimation, winter rye (Secale cereale L.) is able to withstand the formation of extracellular ice at freezing temperatures. We now show, for the first time, that cold-acclimated winter rye plants contain endogenously produced antifreeze protein. The protein was extracted from the apoplast of winter rye leaves, where ice forms during freezing. After partial purification, the protein was identified as antifreeze protein because it modified the normal growth pattern of ice crystals and depressed the freezing temperature of water noncolligatively. 相似文献
10.
Michael C. Iannuzzi Robert C. Stern Francis S. Collins Catherine Tom Hon Noriko Hidaka Theresa Strong Lisa Becker Mitchell L. Drumm Marga B. White Bernard Gerrard Michael Dean 《American journal of human genetics》1991,48(2):227-231
Cystic fibrosis (CF) is a recessive disease caused by mutations in the CF transmembrane conductance regulator (CFTR) gene. We have identified in exon 7 two frameshift mutations, one caused by a two-nucleotide insertion and the other caused by a one-nucleotide deletion; these mutations--CF1154insTC and CF1213delT, respectively, are predicted to shift the reading frame of the protein and to introduce UAA(ochre) termination codons at residues 369 and 368. 相似文献