全文获取类型
收费全文 | 2373篇 |
免费 | 119篇 |
国内免费 | 1篇 |
出版年
2023年 | 7篇 |
2022年 | 10篇 |
2021年 | 31篇 |
2020年 | 22篇 |
2019年 | 31篇 |
2018年 | 35篇 |
2017年 | 26篇 |
2016年 | 53篇 |
2015年 | 67篇 |
2014年 | 82篇 |
2013年 | 156篇 |
2012年 | 171篇 |
2011年 | 157篇 |
2010年 | 98篇 |
2009年 | 76篇 |
2008年 | 159篇 |
2007年 | 147篇 |
2006年 | 127篇 |
2005年 | 163篇 |
2004年 | 148篇 |
2003年 | 146篇 |
2002年 | 140篇 |
2001年 | 30篇 |
2000年 | 41篇 |
1999年 | 34篇 |
1998年 | 34篇 |
1997年 | 28篇 |
1996年 | 26篇 |
1995年 | 24篇 |
1994年 | 18篇 |
1993年 | 9篇 |
1992年 | 26篇 |
1991年 | 20篇 |
1990年 | 23篇 |
1989年 | 15篇 |
1988年 | 15篇 |
1987年 | 11篇 |
1986年 | 6篇 |
1985年 | 9篇 |
1984年 | 12篇 |
1983年 | 9篇 |
1982年 | 7篇 |
1981年 | 7篇 |
1980年 | 5篇 |
1979年 | 4篇 |
1978年 | 3篇 |
1976年 | 4篇 |
1975年 | 3篇 |
1969年 | 5篇 |
1968年 | 5篇 |
排序方式: 共有2493条查询结果,搜索用时 78 毫秒
1.
Hideaki Naoe Tatsuyuki Chiyoda Jo Ishizawa Kenta Masuda Hideyuki Saya Shinji Kuninaka 《Biochemical and biophysical research communications》2013,430(2):757-762
Differentiation of placental trophoblast stem (TS) cells to trophoblast giant (TG) cells is accompanied by transition from a mitotic cell cycle to an endocycle. Here, we report that Cdh1, a regulator of the anaphase-promoting complex/cyclosome (APC/C), negatively regulates mitotic entry upon the mitotic/endocycle transition. TS cells derived from homozygous Cdh1 gene-trapped (Cdh1GT/GT) murine embryos accumulated mitotic cyclins and precociously entered mitosis after induction of TS cell differentiation, indicating that Cdh1 is required for the switch from mitosis to the endocycle. Furthermore, the Cdh1GT/GT TS cells and placenta showed aberrant expression of placental differentiation markers. These data highlight an important role of Cdh1 in the G2/M transition during placental differentiation. 相似文献
2.
3.
4.
Yuichi Takeuchi Megumi Akizuki Hideyuki Shimizu Noriaki Kondo Kiyoshi Sugahara 《Physiologia plantarum》1989,76(3):425-430
Cucumber ( Cucumis sativus L. cv. Natsusairaku 3) seedlings were grown in a growth cabinet under UV-B (290–320 nm) irradiation (equivalent to the UV-B radiation normally incident at Tokyo, 36°N latitude, during clear sky conditions in mid-april on a weighted daily fluence basis) and a UV-B-free control condition. UV-B irradiation inhibited the growth of the cotyledons, i.e. the increase in area, and increase in fresh and dry weights of the cotyledons. The greatest inhibition rate was observed in the increase in area, causing a significant increase in specific leaf weight (the ratio of weight to area). UV-B irradiation had no significant effect on DNA and RNA contents in the cotyledons, but decreased protein content slightly. In contrast, the irradiation reduced the amounts of organic acids and soluble sugars, indicating that primary carbon metabolism was very sensitive to UV-B radiation. UV-B irradiation lowered the photosynthetic activity in the cotyledons without any effect on chlorophyll content and respiratory activity. These results indicate that UV-B radiation at the ambient level may act as a physiological stress in some UV-sensitive plants. 相似文献
5.
Takashi Ooba Hideyuki Hayashi Sachiko Karaki Manabu Tanabe Kyoichi Kano Masafumi Takiguchi 《Immunogenetics》1989,30(2):76-80
The primary structure ofHLA-B51 andHLA-Bw52 suggested thatHLA-B51 was derived fromHLA-Bw52 by the combination of a genetic exchange withHLA-B8 and a point mutation. To investigate the evolution of theHLA-B5 cross reactive group, theHLA-B35 gene was cloned and the primary structure was determined.HLA-B35 is identical toHLA-Bw58 except in the α1 domain. The α1 domain ofHLA-B35 except Bw4/Bw6-associated amino acids is identical to that ofHLA-B51
*, which was suspected to be an intermediate gene betweenHLA-B51 andHLA-Bw52. These data suggest thatHLA-B35 has evolved fromHLA-Bw58 in two steps; an in vivo replacement of the α1 domain withHLA-B51 and genetic exchange with one of theHLA-Bw6 genes. These three genes andHLA-Bw58 are postulated to share a common ancestor. 相似文献
6.
Proalbumin is processed to serum albumin in COS-1 cells transfected with cDNA for rat albumin 总被引:2,自引:0,他引:2
K Oda N Takami T Fujiwara Y Misumi Y Ikehara 《Biochemical and biophysical research communications》1989,163(1):194-200
Synthesis and processing of rat albumin were investigated in COS-1 cells transiently expressing rat albumin. Analysis using isoelectric focusing revealed that serum-type albumin, which is indistinguishable from the counterpart isolated from rat hepatocyte cuture medium, was secreted from the transfected COS-1 cells, indicating that proalbumin is effectively converted into serum albumin in the COS-1 cells, if not completely. Furthermore methylamine was found to cause the diminution of serum albumin released from the cells, substantiating that the proteolytical conversion of proalbumin occurs in the Golgi complex before discharge from the COS-1 cells. 相似文献
7.
Tatsuo Nakahara Makoto Hirano Takashi Matsumoto Toshihide Kuroki Yoshinori Tatebayashi Tetsuyuki Tsutsumi Kouji Nishiyama Hiroaki Ooboshi Kaoru Nakamura Hiroshi Yao Akio Shiraishi Michinori Waki Hideyuki Uchimura 《Neurochemical research》1990,15(6):609-611
DNA and RNA contents in 20 brain regions or nuclei of the rat were determined by a highly sensitive method using high-performance liquid chromatography with electrochemical detection. The high DNA and RNA contents were found in the hypothalamic nuclei, especially the median eminence-arcuate nucleus. These results may be available for the preparation of nucleic acids as the regional control. 相似文献
8.
High density culture of hybridoma cells using a perfusion culture vessel with an external centrifuge 总被引:3,自引:1,他引:2
The influence of centrifugal force on the growth of cells was examined by exposing the cells of the mouse-human hybridoma X87 line to centrifugal force (100–500 G) for ten minutes twice a day and comparing the static culture with that of unexposed cells. In this experiment, both cell proliferation and specific antibody productivity were independent of the centrifugal effect, and gave the same results as in the case of no exposure to centrifugal force. High density cultivation of the mouse-human hybridoma X87 line was obtained by a perfusion system where the cells were separated from the culture medium by continuous centrifugation. In the serum-free culture, the maximum viable cell density exceeded 107 cells/ml, and monoclonal antibody was stably produced for 37 days. The results in this culture were equivalent to those obtained by intermittent centrifugal cell separation from the culture medium, and separation by gravitational settlement. 相似文献
9.
10.
Isogai Akira; Takayama Seiji; Shiozawa Hideyuki; Tsukamoto Chise; Kanbara Takeshi; Hinata Kokichi; Okazaki Keiichi; Suzuki Akinori 《Plant & cell physiology》1988,29(8):1331-1336
S-Glycoproteins (S-locus-specific glycoproteins) in Brassicaspecies are present only in stigmas and thought to play an importantrole in self-incompatibility system. The stigma extract containsalso several other glycoproteins reacting with the antiserumto S-glycoproteins, among which some glycoproteins from S8S8-and S9S9-homozygotes have the same pI value. Both of the glycoproteinswhich were tentatively termed NS8- and NS8S9-glycoproteins,respectively, were isolated and analyzed. Those were revealedto be identical. Its amino acid sequence was homologous withthe S-glycoproteins in Brassica species. The NS-glycoproteinswere expressed at the same time and only in stigma as S-glycoproteins. (Received July 19, 1988; Accepted September 7, 1988) 相似文献