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R. Batchvarova V. Nikolaeva S. Slavov S. Bossolova V. Valkov S. Atanassova S. Guelemerov A. Atanassov H. Anzai 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(5-6):986-989
Six oriental cultivars of tobacco (Nicotiana tabacum L.) were evaluated for transformation and foreign gene expression. Leaf-disc explant tissue was transformed with Agrobacterium tumefaciens strain LBA4404 carrying the plasmid pARK21, which contains NPTII gene and ttr (tabtoxin resistance) gene conferring the resistance to Pseudomonas syringae pv. tabaci. The disease resistance of regenerated plants and segregation of this trait up to R7 progeny were investigated in a greenhouse and under field conditions. Our results indicated that the resistance to Pseudomonas syringae pv. tabaci introduced by transformation is heritable.
Received: 10 June 1997 / Accepted: 31 March 1998 相似文献
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Stoeva Pravda Yankulova Margarita Nikolaeva Velichka Bachvarova Rositza Ivanova Lidia Maiss Edgar Adam Guenter Vulkov Vladimir Guelemerov Spas Atanassov Atanas 《Molecular breeding : new strategies in plant improvement》1998,4(2):155-164
We examined the resistance phenotype of a large number of transgenic tobacco plants originating from 12 commercial (Nicotiana tabacum) cultivars expressing the sense form of the nucleoprotein (N) gene of L3, a Bulgarian isolate of tomato spotted wilt virus (TSWV). The analysis revealed that transgenic plants are completely protected against the homologous L3 isolate of TSWV irrespective of whether or not they contain detectable levels of translational product. The effectiveness of protection against the virus was investigated upon mechanical inoculation under greenhouse conditions and in field trials. Non-segregating resistant lines were selected and the inheritance of the resistance to TSWV was analysed in successive generations (R3–R6). Extensive tests under controlled conditions and two-year field trials proved that the resistance to TSWV is stable in different environments and is a stably inherited trait. 相似文献
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