全文获取类型
收费全文 | 149篇 |
免费 | 2篇 |
国内免费 | 2篇 |
出版年
2021年 | 1篇 |
2020年 | 1篇 |
2018年 | 1篇 |
2017年 | 7篇 |
2016年 | 4篇 |
2015年 | 3篇 |
2014年 | 2篇 |
2013年 | 5篇 |
2012年 | 5篇 |
2011年 | 3篇 |
2010年 | 5篇 |
2009年 | 10篇 |
2008年 | 2篇 |
2007年 | 7篇 |
2006年 | 4篇 |
2005年 | 6篇 |
2004年 | 4篇 |
2003年 | 6篇 |
2002年 | 3篇 |
2001年 | 2篇 |
2000年 | 3篇 |
1999年 | 3篇 |
1997年 | 1篇 |
1996年 | 5篇 |
1995年 | 2篇 |
1994年 | 1篇 |
1992年 | 5篇 |
1991年 | 2篇 |
1990年 | 6篇 |
1989年 | 4篇 |
1988年 | 1篇 |
1987年 | 3篇 |
1986年 | 1篇 |
1985年 | 5篇 |
1984年 | 3篇 |
1983年 | 1篇 |
1980年 | 1篇 |
1979年 | 1篇 |
1978年 | 1篇 |
1977年 | 1篇 |
1975年 | 1篇 |
1974年 | 6篇 |
1973年 | 7篇 |
1972年 | 1篇 |
1971年 | 3篇 |
1970年 | 1篇 |
1969年 | 1篇 |
1968年 | 1篇 |
1967年 | 1篇 |
排序方式: 共有153条查询结果,搜索用时 15 毫秒
1.
Fushitani K; Higashiyama K; Moriyama EN; Imai K; Hosokawa K 《Molecular biology and evolution》1996,13(7):1039-1043
To elucidate phylogenetic relationships among amniotes and the evolution of
alpha globins, hemoglobins were analyzed from the Komodo dragon (Komodo
monitor lizard) Varanus komodoensis, the world's largest extant lizard,
inhabiting Komodo Islands, Indonesia. Four unique globin chains (alpha A,
alpha D, beta B, and beta C) were isolated in an equal molar ratio by high
performance liquid chromatography from the hemolysate. The amino acid
sequences of two alpha chains were determined. The alpha D chain has a
glutamine at E7 as does an alpha chain of a snake, Liophis miliaris, but
the alpha A chain has a histidine at E7 like the majority of hemoglobins.
Phylogenetic analyses of 19 globins including two alpha chains of Komodo
dragon and ones from representative amniotes showed the following results:
(1) The a chains of squamates (snakes and lizards), which have a glutamine
at E7, are clustered with the embryonic alpha globin family, which
typically includes the alpha D chain from birds; (2) birds form a sister
group with other reptiles but not with mammals; (3) the genes for embryonic
and adult types of alpha globins were possibly produced by duplication of
the ancestral alpha gene before ancestral amniotes diverged, indicating
that each of the present amniotes might carry descendants of the two types
of alpha globin genes; (4) squamates first split off from the ancestor of
other reptiles and birds.
相似文献
2.
3.
4.
Nadolinskaia N. I. Zamakhaev M. V. Shumkov M. S. Armianinova D. K. Karpov D. S. Goncharenko A. V. 《Applied Biochemistry and Microbiology》2021,57(4):421-425
Applied Biochemistry and Microbiology - This work describes a modification of the pRH2521 vector of the pRH2502/pRH2521 system for CRISPR-dCas9-mediated RNA interference. The modification enabled... 相似文献
5.
Ilja I. Brusentsov Alexey V. Katokhin Irina V. Brusentsova Sergei V. Shekhovtsov Sergei N. Borovikov Grigoriy G. Goncharenko Lyudmila A. Lider Boris V. Romashov Olga T. Rusinek Samat K. Shibitov Marat M. Suleymanov Andrey V. Yevtushenko Viatcheslav A. Mordvinov 《PloS one》2013,8(4)
Opisthorchis felineus or Siberian liver fluke is a trematode parasite (Opisthorchiidae) that infects the hepato-biliary system of humans and other mammals. Despite its public health significance, this wide-spread Eurasian species is one of the most poorly studied human liver flukes and nothing is known about its population genetic structure and demographic history. In this paper, we attempt to fill this gap for the first time and to explore the genetic diversity in O. felineus populations from Eastern Europe (Ukraine, European part of Russia), Northern Asia (Siberia) and Central Asia (Northern Kazakhstan). Analysis of marker DNA fragments from O. felineus mitochondrial cytochrome c oxidase subunit 1 and 3 (cox1, cox3) and nuclear rDNA internal transcribed spacer 1 (ITS1) sequences revealed that genetic diversity is very low across the large geographic range of this species. Microevolutionary processes in populations of trematodes may well be influenced by their peculiar biology. Nevertheless, we suggest that lack of population genetics structure observed in O. felineus can be primarily explained by the Pleistocene glacial events and subsequent sudden population growth from a very limited group of founders. Rapid range expansion of O. felineus through Asian and European territories after severe bottleneck points to a high dispersal potential of this trematode species. 相似文献
6.
Konstantinos Tsekouras Igor Goncharenko Michael E. Colvin Kerwyn Casey Huang Ajay Gopinathan 《PloS one》2013,8(6)
Although targeting of cancer cells using drug-delivering nanocarriers holds promise for improving therapeutic agent specificity, the strategy of maximizing ligand affinity for receptors overexpressed on cancer cells is suboptimal. To determine design principles that maximize nanocarrier specificity for cancer cells, we studied a generalized kinetics-based theoretical model of nanocarriers with one or more ligands that specifically bind these overexpressed receptors. We show that kinetics inherent to the system play an important role in determining specificity and can in fact be exploited to attain orders of magnitude improvement in specificity. In contrast to the current trend of therapeutic design, we show that these specificity increases can generally be achieved by a combination of low rates of endocytosis and nanocarriers with multiple low-affinity ligands. These results are broadly robust across endocytosis mechanisms and drug-delivery protocols, suggesting the need for a paradigm shift in receptor-targeted drug-delivery design. 相似文献
7.
Karpov D. S. Domashin A. I. Kotlov M. I. Osipova P. G. Kiseleva S. V. Seregina T. A. Goncharenko A. V. Mironov A. S. Karpov V. L. Poddubko S. V. 《Molecular Biology》2020,54(1):119-127
Molecular Biology - Bacillus subtilis bacteria play an important role in veterinary medicine, medicine, and biotechnology, and the permanently growing demand for biotechnological products fuels the... 相似文献
8.
Navolotskaia EV Vanina VI Zargarova TA Goncharenko EN Kudriashova NIu Akhalaia MIa Sadovnikov VB Semushkina SG Kolobov AA Kampe-Nemm EA Iurovskiĭ VV Lipkin VM 《Bioorganicheskaia khimiia》2004,30(4):350-355
The effect of immunocortin, an ACTH-like decapeptide VKKPGSSVKV corresponding to the 11-20 sequence of the variable part of the human IgG1 heavy chain on the content of 11-hydroxycorticosteroids (CS) in rat adrenal glands and blood serum in vivo was studied. An intramuscular injection of immunocortin at a dose of 10 microg/kg was found in an hour to induce a twofold decrease in CS content in the adrenal glands and a 1.8-fold increase in the blood serum CS content. At the same time, an immunocortin dose of 100 microg/kg exerted practically no effect on the CS content and its dose of 1000 microg/kg increased the CS content both in adrenal glands and in blood serum by 1.6 and 2.2 times, respectively. Four hours after the injection of any of the three doses of immunocortin, the CS content in adrenal glands did not differ from the control value, and after 24 h the content decreased threefold. Immunocortin was shown to be bound by the ACTH receptors in the membranes of the rat adrenal cortex with a high affinity and specificity (inhibiting the specific binding of 125I-labeled ACTH-(11-24) peptide with Ki of 1.2 nM). 相似文献
9.
Maslova MV Maklakova AS Sokolova NA Ahmarin IP Goncharenko EN Krushinskaia IaV 《Rossi?skii fiziologicheski? zhurnal imeni I.M. Sechenova / Rossi?skaia akademiia nauk》2002,88(2):184-190
Ante- and postnatal acute hypoxia significantly aggravated the postnatal development. The posthypoxic behaviour patterns included hyperactivity and training ability inhibition typical for the attention deficit syndrome. A preventive injection of peptide constellation significantly improved the posthypoxic postnatal development and abolished the most of the negative modifications of behavioural patterns. 相似文献
10.
The oligomycin-sensitive component of the rate of oxygen consumption by rat thymocytes increased by 22% after X-irradiation. The postirradiation increase in the thymocyte respiration rate was connected with the impairment of a balance between sodium, potassium and calcium and with the protein synthesis activation, and was independent of lymphokine production by lipoxygenase. Irradiation was ineffective with respect to electron transport in the respiratory chain and electrogenic leakages through the inner membrane of thymocyte mitochondria. 相似文献