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排序方式: 共有61条查询结果,搜索用时 236 毫秒
1.
Servaas Visser Charles J. Slangen Fred A. Exterkate Gerrie J. C. M. de Veer 《Applied microbiology and biotechnology》1988,29(1):61-66
Summary The specificity of a cell wall proteinase (PI) from Streptococcus cremoris strain HP in its action on bovine -casein was determined. To this end an enzymic digest (pH 6.2; 15° C) of -casein was brought to pH 4.6 and the soluble fraction separated by semi-preparative reversed-phase HPLC. Purified peptides were analyzed by amino acid and end-group analysis. Ten chromatographic components were identified, which together accounted for at least seven cleavage sites all being located in the C-terminal fifty-residue part of -casein. In five cases it concerned a Gln-X or X-Gln peptide linkage. The specificity of this proteinase from S. cremoris HP shows similarity to that reported for a cell wall proteinase from S. lactis NCDO 763 in its action on -casein.Presented at the second FEMS Symposium on Lactic Acid Bacteria held in 1987 at Wageningen, Netherlands 相似文献
2.
Comparative Study of Action of Cell Wall Proteinases from Various Strains of Streptococcus cremoris on Bovine αs1-, β-, and κ-Casein 下载免费PDF全文
Servaas Visser Fred A. Exterkate Charles J. Slangen Gerrie J. C. M. de Veer 《Applied microbiology》1986,52(5):1162-1166
Partially purified cell wall proteinases of eight strains of Streptococcus cremoris were compared in their action on bovine αs1-, β-, and κ-casein, as visualized by starch gel electrophoresis, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and thin-layer chromatography. Characteristic degradation profiles could be distinguished, from which the occurrence of two proteinases, represented by strain HP and strain AM1, was concluded. The action of the HP-type proteinase P1 (also detectable in strains Wg2, C13, and TR) was established by electrophoretic methods to be directed preferentially towards β-casein. The AM1-type proteinase PIII (also detectable in strain SK11) was also able to degrade β-casein, but at the same time split αs1- and κ-casein more extensively than did PI. Strain FD27 exhibited mainly PI activity but also detectable PIII degradation characteristics. The cell wall proteinase preparation of strain E8 showed low PI as well as low PIII activity. All proteinase preparations produced from κ-casein positively charged degradation products with electrophoretic mobilities similar to those of degradation products released by the action of the milk-clotting enzyme chymosin. The differences between PI and PIII in mode of action, as detected by gel electrophoresis and thin-layer chromatography, were reflected by the courses of the initial degradation of methyl-14C-labeled β-casein and by the effect of αs1- plus κ-casein on these degradations. The results are discussed in the light of previous comparative studies of cell wall proteinases in strains of S. cremoris and with respect to the growth of this organism in milk. 相似文献
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Obesity and Age‐Related Changes in Markers of Oxidative Stress and Inflammation Across Four Generations 下载免费PDF全文
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Fina A. S Kurreeman Leonid Padyukov Rute B Marques Steven J Schrodi Maria Seddighzadeh Gerrie Stoeken-Rijsbergen Annette H. M van der Helm-van Mil Cornelia F Allaart Willem Verduyn Jeanine Houwing-Duistermaat Lars Alfredsson Ann B Begovich Lars Klareskog Tom W. J Huizinga Rene E. M Toes 《PLoS medicine》2007,4(12)
5.
Boon AC De Mutsert G Fouchier RA Sintnicolaas K Osterhaus AD Rimmelzwaan GF 《Journal of immunology (Baltimore, Md. : 1950)》2004,172(7):4435-4443
To study whether individual HLA class I alleles are used preferentially or equally in human virus-specific CTL responses, the contribution of individual HLA-A and -B alleles to the human influenza virus-specific CTL response was investigated. To this end, PBMC were obtained from three groups of HLA-A and -B identical blood donors and stimulated with influenza virus. In the virus-specific CD8(+) T cell population, the proportion of IFN-gamma- and TNF-alpha-producing cells, restricted by individual HLA-A and -B alleles, was determined using virus-infected C1R cells expressing a single HLA-A or -B allele for restimulation of these cells. In HLA-B*2705- and HLA-B*3501-positive individuals, these alleles were preferentially used in the influenza A virus-specific CTL response, while the contribution of HLA-B*0801 and HLA-A*0101 was minor in these donors. The magnitude of the HLA-B*0801-restricted response was even lower in the presence of HLA-B*2705. C1R cells expressing HLA-B*2705, HLA-A*0101, or HLA-A*0201 were preferentially lysed by virus-specific CD8(+) T cells. In contrast, the CTL response to influenza B virus was mainly directed toward HLA-B*0801-restricted epitopes. Thus, the preferential use of HLA alleles depended on the virus studied. 相似文献
6.
Boon AC de Mutsert G van Baarle D Smith DJ Lapedes AS Fouchier RA Sintnicolaas K Osterhaus AD Rimmelzwaan GF 《Journal of immunology (Baltimore, Md. : 1950)》2004,172(4):2453-2460
In the present study, the recognition of epitope variants of influenza A viruses by human CTL was investigated. To this end, human CD8(+) CTL clones, specific for natural variants of the HLA-B*3501-restricted epitope in the nucleoprotein (NP(418-426)), were generated. As determined in (51)Cr release assays and by flow cytometry with HLA-B*3501-peptide tetrameric complexes, CTL clones were found to be specific for epitopes within one subtype or cross-reactive with heterosubtypic variants of the epitope. Using eight natural variants of the epitope, positions in the 9-mer important for T cell recognition and involved in escape from CTL immunity were identified and visualized using multidimensional scaling. It was shown that positions 4 and 5 in the 9-mer epitope were important determinants of T cell specificity. The in vivo existence of CD8(+) cells cross-reactive with homo- and heterosubtypic variants of the epitope was further confirmed using polyclonal T cell populations obtained after stimulation of PBMC with different influenza A viruses. Based on the observed recognition patterns of the clonal and polyclonal T cell populations and serology, it is hypothesized that consecutive infections with influenza viruses containing different variants of the epitope select for cross-reactive T cells in vivo. 相似文献
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J W Callahan M Khalil J Gerrie 《Biochemical and biophysical research communications》1974,58(2):385-390
Isoenzymes of sphingomyelinase have been resolved by isoelectric focusing. The two major species (I and II) in human liver have distinct isoelectric points, pH optima and Km values. Liver from Niemann-Pick disease Type C contained isoenzyme I (pI 4.6) while isoenzyme II (pI 5.2) was absent. The absence of isoenzyme II likely constitutes the genetic defect in this disease. 相似文献
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