Listeriosis in Sheep

A comparison was made between a hay fed group, consisting of 23 ewes, and a grass silage fed group of 22 ewes, all pregnant. Excretion of Listeria monocytogenes (Lm) in the faeces and milk, antibody titres in sera and whey and delayed hypersensitivity against Lm, and several blood components were determined. The animals had previously been exposed to Lm, and Lm was isolated from the faeces from several animals when the experiment started. No significant difference in number of excretors between the 2 groups was found during the experimental period. The haemagglutination titres in both sera and whey were low and on the same level in both groups. The titres were higher in animals with 1 foetus than in animals with more than 1 foetus. In the first part of the experimental period the silage group had a reduced number of lymphocytes, lower total serum protein values and higher serum iron values, compared with the hay group. The silage group also had a stronger delayed hypersensitivity reaction against Lm than the hay group, and in the silage group the reaction was significantly stronger in ewes with 3 or more foetuses than in ewes with 1 foetus. In conclusion, the combined effect of some of the changes found in animals fed grass silage may leave them more susceptible to infections.     

Some dasyclad algae of the sinemurian from the north-western iberian chains (Spain)

In a section of the Liassic southwest of the village of Préjano (Prov. Logroño) Sinemurian Dasyclad algae were found for the first time in the “Bankkalk-Series” of the “Carniolas-Formation” which is not dated up to that time. Six species,Dissocladella lucasi (Cros & Lemoine),Dissocladella iberica nov. sp.,Dissocladella ebroensis nov. sp.,Sestrosphaera liasina Pia,Gyroporella retica (Zanin) andMacroporella nov. sp. aff. sturi Bystricky are described.     

The mongolian game Norobo or Cindi

Current debates over the concept of culture are examined, especially with regard to the relationship of culture to bounded collectivities, human nature, and individuality; present‐day views are seen against the background of back‐and‐forth swings in the history of anthropological thought. Recent theorizing about cultural acquisition is also considered.     

Separation of plant membranes by electromigration techniques

The review focuses on the multiple separating regimes that offers the free flow electrophoresis technique: free flow zone electrophoresis, isoelectric focusing, isotachophoresis, free flow step electrophoresis. Also, the feasibility to apply either interval or continuous flow electrophoresis is evaluated. The free flow zone electrophoresis regime is generally selected for the separation of cells, organelles and membranes while the other regimes find their largest fields of applications in the purification of proteins and peptides. The latter regimes present the highest resolution efficiency. Therefore, a large part of this review is devoted to the applicabilities of these different regimes to the purification of organelles and membrane vesicles at the preparative scale. Recent developments, both in instrumentation and procedures, are described. The major achievements in plant membrane fractionation obtained with free flow electrophoresis are outlined. The related procedures are both analytical and preparative: they separate tonoplast and plasma membrane simultaneously from the same homogenate, they discriminate for one type of membrane vesicles of opposite orientation, and process large quantities of membrane material by reason of the continuous flow mode. Recent advances using electromigration techniques that permit confirmation of the dynamic state of membranes, characterisation of complex membrane-dependent functions and discovery of new membrane-localised activities are presented.