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Site-specific endonucleolytic cleavages and the regulation of stability of E. coli ompA mRNA 总被引:53,自引:0,他引:53
The stability of ompA mRNA is growth-rate dependent. We show that the 5' noncoding region of this mRNA provides a target for site-specific endonucleases. The rate of degradation of ompA mRNA parallels the rate of these endonucleolytic cleavages, implying that endonucleolytic rather than exonucleolytic attack is the initial step in ompA mRNA degradation. Thus the 5' noncoding region appears to be a determinant of mRNA stability, and endonucleolytic cleavages in the 5' noncoding region may well regulate expression of the ompA gene. 相似文献
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Biochemical and serological evidence for an RNase E-like activity in halophilic Archaea. 总被引:2,自引:0,他引:2 下载免费PDF全文
Endoribonuclease RNase E appears to control the rate-limiting step that mediates the degradation of many mRNA species in bacteria. In this work, an RNase E-like activity in Archaea is described. An endoribonucleolytic activity from the extreme halophile Haloarcula marismortui showed the same RNA substrate specificity as the Escherichia coli RNase E and cross-reacted with a monoclonal antibody raised against E. coli RNase E. The archaeal RNase E activity was partially purified from the extreme halophilic cells and shown, contrary to the E. coli enzyme, to require a high salt concentration for cleavage specificity and stability. These data indicate that a halophilic RNA processing enzyme can specifically recognize and cleave mRNA from E. coli in an extremely salty environment (3 M KCI). Having recently been shown in mammalian cells (A. Wennborg, B. Sohlberg, D. Angerer, G. Klein, and A. von Gabain, Proc. Natl. Acad. Sci. USA 92:7322-7326, 1995), RNase E-like activity has now been identified in all three evolutionary domains: Archaea, Bacteria, and Eukarya. This strongly suggests that mRNA decay mechanisms are highly conserved despite quite different environmental conditions. 相似文献
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Using certain morphological methods, relief peculiarities of the cardiac ventricle chambers, form and extent of the intertrabecular spaces, connections with the myocardial blood bed have been studied in 92 human hearts. Foramina, fissurae and excavations on the internal surface of the ventricles are the beginnings of the intertrabecular spaces, in their deep parts elements of the microcirculatory blood bed of the myocardium have openings. As demonstrate serial sections, there are not any immediate anastomoses between the myocardial arteries, veins and the intertrabecular spaces. This fact does not confirm the existing opinion that the smallest cardiac veins (Viessen-Thebesian vessels) belong to the arterio-venous anastomoses. A propose is made to use the term "the smallest cardiac veins" only to the veins that directly open into the auricular chambers. 相似文献
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T Henics E Nagy H J Oh P Csermely A von Gabain J R Subjeck 《The Journal of biological chemistry》1999,274(24):17318-17324
In this study, in vitro RNA binding by members of the mammalian 70-kDa heat shock protein (Hsp) family was examined. We show that Hsp/Hsc70 and Hsp110 proteins preferentially bound AU-rich RNA in vitro. Inhibition of RNA binding by ATP suggested the involvement of the N-terminal ATP-binding domain. By using deletion mutants of Hsp110 protein, a diverged Hsp70 family member, RNA binding was localized to the N-terminal ATP-binding domain of the molecule. The C-terminal peptide-binding domain did not bind RNA, but its engagement by a peptide substrate abrogated RNA binding by the N terminus of the protein. Interestingly, removal of the C-terminal alpha-helical structure or the alpha-loop domain unique to Hsp110 immediately downstream of the peptide-binding domain, but not both, resulted in considerably increased RNA binding as compared with the wild type protein. Finally, a 70-kDa activity was immunoprecipitated from RNA-protein complexes formed in vitro between cytoplasmic proteins of human lymphocytes and AU-rich RNA. These findings support the idea that certain heat shock proteins may act as RNA-binding entities in vivo to guide the appropriate folding of RNA substrates for subsequent regulatory processes such as mRNA degradation and/or translation. 相似文献
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