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Frugis G Giannino D Mele G Nicolodi C Chiappetta A Bitonti MB Innocenti AM Dewitte W Van Onckelen H Mariotti D 《Plant physiology》2001,126(4):1370-1380
Leaves are specialized organs characterized by defined developmental destiny and determinate growth. The overexpression of Knotted1-like homeobox genes in different species has been shown to alter leaf shape and development, but a definite role for this class of genes remains to be established. Transgenics that overexpress Knotted1-like genes present some traits that are characteristic of altered cytokinin physiology. Here we show that lettuce (Lactuca sativa) leaves that overexpress KNAT1, an Arabidopsis kn1-like gene, acquire characteristics of indeterminate growth typical of the shoot and that this cell fate change is associated with the accumulation of specific types of cytokinins. The possibility that the phenotypic effects of KNAT1 overexpression may arise primarily from the modulation of local ratios of different cytokinins is discussed. 相似文献
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Zuo J Niu QW Frugis G Chua NH 《The Plant journal : for cell and molecular biology》2002,30(3):349-359
Formation of somatic embryos in plants is known to require high concentrations of auxin or 2,4-dichlorophenoxyacetic acid (2,4-D), which presumably acts to trigger a signalling cascade. However, very little is known about the molecular mechanism that mediates the vegetative-to-embryogenic transition. We have employed a genetic approach to dissect the signal transduction pathway during somatic embryogenesis. In a functional screen using a chemical-inducible activation-tagging system, we identified two alleles of Arabidopsis gene PGA6 whose induced overexpression caused high-frequency somatic embryo formation in all tissues and organs tested, without any external plant hormones. Upon inducer withdrawal, all these somatic embryos were able to germinate directly, without any further treatment, and to develop into fertile adult plants. PGA6 was found to be identical to WUSCHEL (WUS), a homeodomain protein previously shown to be involved in specifying stem cell fate in shoot and floral meristems. Transgenic plants carrying an estradiol-inducible XVE-WUS transgene can phenocopy pga6-1 and pga6-2. Our results suggest that WUS/PGA6 also plays a key role during embryogenesis, presumably by promoting the vegetative-to-embryogenic transition and/or maintaining the identity of the embryonic stem cells. 相似文献
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Giannino D Mele G Cozza R Bruno L Testone G Ticconi C Frugis G Bitonti MB Innocenti AM Mariotti D 《Journal of experimental botany》2003,54(393):2623-2633
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Imerio Capone Giovanna Frugis Paolo Costantino Maura Cardarelli 《Plant molecular biology》1994,25(4):681-691
Selective gene expression in different populations of cells of the root apex of transgenic tobacco could be evidenced by means of GUS constructs with deletions of the rolB promoter and fusions with the CaMV 35S minimal promoter. Five regulatory regions have been broadly identified in the rolB 5 non-coding region. The presence of all five domains (A to E) directs gene expression in the root cap, in the protoderm and in the different tissues within the root meristematic region: the dermatocalyptrogen, the cortex and the vascular cylinder. Deletion of domain A (–623 to –471) selectively suppresses expression in non-meristematic cells, i.e. the root cap and the protoderm. Deletion of either domain B (–341 to –306) or E (80 bp around the TATA box) causes loss of expression in all cells of the root apex: constructs C+D+E, B+C+D, B+C are inactive. Domain D (70 bp around the CAAT box) is necessary for gene expression in the dermatogen and in meristematic cells of the cortex but not in the innermost meristematic layer: construct B+C+E is active only in vascular meristematic cells. Domain C (–216 to –158) seems to have a double regulatory role as construct B+E is no longer expressed in meristematic cells of the vascular cylinder but is very active in the protoderm. Constructs allowing gene expression in meristematic cells are also inducible by auxin in leaf protoplasts, while activation of the regulatory elements necessary for gene expression in the non-meristematic cells of the root apex do not seem to depend upon the hormone. The connection between auxin induction and meristematic expression is discussed. 相似文献
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Being sessile organisms, plants usually have little control over their immediate growth environment. Responses to environmental and developmental factors need to be rapid and finely coordinated to trigger the necessary morphological and metabolic changes that ensure plant survival and growth. Many of these adaptive responses are mediated by plant hormones. Recent work has shown that ubiquitin-mediated proteolysis plays an important regulatory role in hormone signaling. 相似文献
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Zusammenfassung Vertikale Verteilung von Mönchs- und Gartengrasmücken in der Vegetation. — In Norditalien ließen sich jahreszeitliche Unterschiede in der vertikalen Verteilung von Mönchsgrasmücken mit Hilfe standardisierter Netzfänge feststellen. Im Herbst kann sich aus der Ähnlichkeit des Verteilungsmusters zwischen Garten- und Mönchsgrasmücke interspezifische Konkurrenz ergeben. Die gefundenen Verteilungsmuster gleichen solchen aus Gegenden mit ganz anderer Artenzusammensetzung der Vegetation.
Summary Vertical distribution patterns of Blackcap and Garden Warbler within homogeneous vegetation have been investigated through standardized mist-netting in Northern Italy. A clear seasonal difference in the use of the vegetation has been shown in the Blackcap. Some kind of interspecific competition can be expected on the basis of the similarity in the autumn vertical distribution of the two species; both distribution patterns are very similar to those found by other authors in areas with different plant species composition.相似文献