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Ecological genetics of Italian peach‐potato aphid (Myzus persicae) populations in relation to geography,dispersal and insecticide resistance as studied using microsatellite and resistance markers 下载免费PDF全文
Valentina Monti Hugh D. Loxdale Michele Cesari Milo Frattini Michela Panini Emanuele Mazzoni Gian Carlo Manicardi 《Agricultural and Forest Entomology》2016,18(4):376-389
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Higher plasma catecholamine and metabolite levels in the early phase of nonsegmental vitiligo 总被引:2,自引:0,他引:2
Cucchi ML Frattini P Santagostino G Orecchia G 《Pigment cell research / sponsored by the European Society for Pigment Cell Research and the International Pigment Cell Society》2000,13(1):28-32
The etiology of vitiligo is still being debated, although neural factors seem to play a pivotal role in its pathogenesis. In our search for a link between vitiligo and the activity of monoaminergic systems, we used high-pressure liquid chromatography and electrochemical detector (HPLC-ED) methods to measure the plasma levels of the following substances in 35 healthy subjects and in 70 patients suffering from nonsegmental vitiligo at the different stages of the disease: catecholamines [norepinephrine (NE), epinephrine (E), and dopamine (DA)], their precursor 3,4-dihydroxyphenylalanine (DOPA), their metabolites [3-methoxy-4-hydroxyphenylglycol (MHPG), normetanephrine (NMN), metanephrine (MN), and homovanillic acid (HVA)], and 5-hydroxyindolacetic acid (5-HIAA) as the major metabolite of serotonin. We found that the levels of NE, E, NMN, MN, HVA, and 5-HIAA were significantly higher in patients compared to controls. The patients at an active phase of the disease (n = 49/70) showed significantly higher levels of NE, NMN, MHPG, and HVA than ones at a stable phase. The patients with progressive vitiligo and at its more recent onset (< 1 year) showed significantly increased levels of E, NE, and MN in comparison with longer-term sufferers. No significant differences were observed when the patients were subdivided according to the type of vitiligo or their age at its onset. The higher catecholamine and metabolite levels in the early phase of the disease may reflect increased activity by monoaminergic systems, probably due to stressful events, including the onset of vitiligo itself. 相似文献
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McCann MD Lim GF Manni ML Estes J Klapec KA Frattini GD Knarr RJ Gratton JL Serra MJ 《RNA (New York, N.Y.)》2011,17(1):108-119
Thirty-one RNA duplexes containing single-nucleotide bulge loops were optically melted in 1 M NaCl, and the thermodynamic parameters ΔH°, ΔS°, ΔG°(37), and T(M) for each sequence were determined. The bulge loops were of the group II variety, where the bulged nucleotide is identical to one of its nearest neighbors, leading to ambiguity as to the exact position of the bulge. The data were used to develop a model to predict the free energy of an RNA duplex containing a single-nucleotide bulge. The destabilization of the duplex by the bulge was primarily related to the stability of the stems adjacent to the bulge. Specifically, there was a direct correlation between the destabilization of the duplex and the stability of the less stable duplex stem. Since there is an ambiguity of the bulge position for group II bulges, several different stem combinations are possible. The destabilization of group II bulge loops is similar to the destabilization of group I bulge loops, if the second least stable stem is used to predict the influence of the group II bulge. In-line structure probing of the group II bulge loop embedded in a hairpin indicates that the bulged nucleotide is the one positioned farther from the hairpin loop. 相似文献
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Enrico Caruso Paola D''Arrigo Sara Frattini Giuseppe Pedrocchi-Fantoni Stefano Servi 《Journal of Molecular Catalysis .B, Enzymatic》2001,11(4-6):427-432
Crude lipases are used for the resolution of racemic ketals advanced intermediates in the synthesis of cis-terconazole and cis-ketoconazole. Lipase from Aspergillus niger allows to obtain the (2R, 4R)-enantiomer of the imidazole-substituted ketal in good ee at low conversion. The addition of adsorbing resins improves the efficiency to interesting ee values for practical applications. The compound is transformed into (2R, 4S)-terconazole. The survived ester gives access to the other enantiomer. 相似文献
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Cinzia Barbieri Enrico Caruso Paola D''Arrigo Sara Frattini Giuseppe Pedrocchi-Fantoni Stefano Servi 《Journal of Molecular Catalysis .B, Enzymatic》2001,11(4-6):487-490
o-, m-, p-Bis-phenylacetic and -bis-phenoxyacetic acid esters with solketal are prepared and submitted to enzymatic hydrolysis with penicillin V (PVA) and G (PGA) amidases. While the para-isomers are recovered unchanged, ortho- and meta-bis-esters are completely hydrolysed. PVA shows a reversed substrate specificity, hydrolysing phenylacetates faster than its natural substrate. The use of the bis-acids as alcohol-protecting groups is proposed. 相似文献