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1.
Bradyrhizobium japonicum Survival in and Soybean Inoculation with Fluid Gels 总被引:2,自引:0,他引:2
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The utilization of gels, which are used for fluid drilling of seeds, as carriers of Bradyrhizobium japonicum for soybean (Glycine max (L.) Merr.) inoculation was studied. Gels of various chemical composition (magnesium silicate, potassium acrylate-acrylamide, grafted starch, and hydroxyethyl cellulose) were used, although the hydroxyethyl cellulose gels were more extensively investigated. Gel inocula were prepared by mixing gel powder with liquid cultures of B. japonicum (2% [wt/vol]). The population of B. japonicum USDA 110 did not change in each gel type during 8 days of incubation at 28°C. These fluid gels were prepared with late-exponential-growth-phase cells that were washed and suspended in physiological saline. Mid-exponential-growth-phase B. japonicum USDA 110, 123, and 138 grew in cellulose gels prepared with yeast extract-mannitol broth as well as or better than in yeast extract-mannitol broth alone for the first 10 days at 28°C. Populations in these cellulose gels after 35 days were as large as when the gels had originally been prepared, and survival occurred for at least 70 days. Soybeans grown in sand in the greenhouse had greater nodule numbers, nodule weights, and top weights with gel inoculants compared with a peat inoculant. In soil containing 103 indigenous B. japonicum per g of soil, inoculation resulted in increased soybean nodule numbers, nodule weights, and top weights, but only nodule numbers were greater with gel than with peat inoculation. The gel-treated seeds carried 102 to 103 more bacteria per seed (107 to 108) than did the peat-treated seeds. 相似文献
2.
Current inputs of organic materials to cropped lands on sandy Alfisols and Entisols in Sahelian West Africa are insufficient to arrest soil organic matter (SOM) decline. Crop residues and green manures require proper management in order to maximize their contribution to nutrient supply and SOM maintenance. The objectives of this study were to quantify the rates of C and N mineralization from cowpea (Vigna unguiculata (L.) Walp.) green manure, cowpea residue, and millet (Pennisetum glaucum (L.) R.Br.) residue under field conditions in Niger and to determine the effect of these organic amendments on pearl millet yield. Millet was grown (1) as sole crop, (2) as intercrop with cowpea, (3) as intercrop with cowpea that was incorporated as green manure during the second half of the growing season, (4) with incorporated cowpea residue (2000 kg ha–1), (5) with millet residue mulch (3000 kg ha–1), and (6) with N fertilizer. Carbon loss as CO2 from soil with and without organic amendment was measured three times per week during the growing season. Nitrogen fertilizer increased millet yield only in a year with a favorable rainfall distribution. Cowpea grown in intercrop with millet during the first part of the growing season and subsequently incorporated as green manure between millet rows increased millet grain yield in a year with sufficient early rainfall, which could be attributed to the rapid rate of decomposition and nutrient release during the first 3 weeks after incorporation. In a year with limited early rainfall, however, densely planted green manure cowpeas competed for water and nutrients with the growing millet crop. Incorporated cowpea residue and millet residue mulch increased millet yield. Surface applied millet residue had high rates of decomposition only during the first 3 days after a rainfall event, with 34% of the millet residue C lost as CO2 in one rainy season. Recovery of undecomposed millet residue at the end of the rainy season was related to presence or absence of termites, but not to seasonal C loss. Millet residue mulch increased soil organic C content of this sandy Alfisol in Niger. Cowpea and millet residues had a greater effect on SOM and millet yield than cowpea green manure due to their greater rate of application and slower rate of decomposition. 相似文献
3.
Prediction of soil organic C sequestration with adoption of various conservation agricultural management approaches is needed to meet the emerging market for environmental services provided by agricultural land stewardship. The soil conditioning index (SCI) is a relatively simple model used by the USDA?CNatural Resources Conservation Service to predict qualitative changes in soil organic matter. Our objective was to develop a quantitative relationship between soil organic C derived from published field studies in the southeastern USA and SCI scores predicted from matching management conditions. We found that soil organic C sequestration (at 20?±?5 cm depth) could be reliably related to SCI across a diversity of studies in the region using the regression slope: 1.65 Mg C ha?1 SCI?1 [which translated into a rate of 0.25?±?0.04 Mg C ha?1 yr?1 SCI?1 (mean±standard error of 31 slope estimates)]. The calibration of soil organic C on SCI scores will allow SCI to become a quantitative tool for natural resource professionals to predict soil organic C sequestration for farmers wanting to adopt conservation practices. 相似文献
4.
Cowpea [Vigna unguiculata (L). Walp.] has great potential as green manure due to its rapid N accumulation and efficient N2 fixation. The objective of this study was to measure the rate of N mineralization from cowpea plant parts harvested at onset
of flowering (5 weeks) and mid pod-fill (7 weeks) under near optimum conditions. Cowpeas were grown in a greenhouse and supplied
with 15NH4
15NO3 to isotopically label tissue. Cowpea leaves, stems, and roots were incorporated into a sandy soil (Psammentic Paleustalf)
and net N mineralized was measured several times during a 10 week incubation. The amount of N accumulated in 7-week old cowpeas
was more than double that in 5-week old cowpeas. The portion of N mineralized after 10 weeks was 24% for 5-week old cowpeas
and 27% for 7-week old cowpeas. The rate of N mineralization from leaves and stems increased with plant age, but decreased
for roots. The amount of N mineralized from 7-week old cowpeas was more than double (235%) that from 5-week old cowpeas due
to greater N accumulation and a more rapid rate of N mineralization of the more mature cowpeas. The greatest amount of N was
released from leaves, which amounted to 74 and 65% of total N mineralization from 5- and 7-week old cowpeas, respectively.
The percentage of N mineralized by 10 weeks was linearly related to the tissue N concentration of the plant parts and to their
C/N ratio. These relationships allow a quick estimation of the amount of N that would mineralize from cowpea residues incorporated
into soil based on their N concentration or C/N ratio. 相似文献
5.
Tillage,crop residue,legume rotation,and green manure effects on sorghum and millet yields in the semiarid tropics of Mali 总被引:3,自引:0,他引:3
Kouyaté Zoumana Franzluebbers Kathrin Juo Anthony S.R. Hossner Lloyd R. 《Plant and Soil》2000,225(1-2):141-151
Alternative soil management practices are needed in semi-arid West Africa to sustain soil fertility and cereal production
while reducing the need for extended fallow periods and chemical fertilizers. An experiment was conducted at the Cinzana Station
near Segou, Mali to assess the effects of tillage, crop residue incorporation and legume rotation on the growth and yield
of sorghum (Sorghum bicolor L. Moench) and pearl millet (Pennisetum glaucum L.) for a period of eight years on a loamy sand and a loam soil. The following treatments were compared under tied ridging
and the traditional open ridging: continuous cereal with crop residue removed, continuous cereal with crop residue incorporated,
cereal in rotation with cowpea (Vigna unguiculata (L.) Waip.), cereal in rotation with sesbania (Sesbania rostrata Bremek. & Oberm.), and cereal in rotation with dolichos (Dolichos lablab L.). Legumes in rotation were incorporated as green manures except cowpea which was removed after each harvest. Tied ridging
improved cereal grain yield from 1022 kg ha−1 with open ridging to 1091 kg ha−1 on the loamy sand and from 1554 kg ha−1 to 1697 kg ha−1 on the loam, when averaged across management regimes and years of cropping. Incorporation of cereal residue at the beginning
of the rainy season every other year had only small and inconsistent effects on cereal yield. Rotation with cowpea increased
cereal grain and stover yields by 18 and 25%, respectively, on the loamy sand, and by 23% and 27%, respectively, on the loam
compared to continuous cereal, when averaged across tillage regimes and years. Sesbania and dolichos performed similarly as
green manures on both soils. Incorporation of these legumes as green manure at the end of the rainy season increased cereal
grain and stover yields by 37% and 49%, respectively, on the loamy sand, and by 27% and 30%, respectively, on the loam, compared
to cereal monoculture without organic amendment, when averaged across tillage regimes and years. A significant linear increase
in cereal yield was observed during the eight years of the study on the loam soil when sesbania and dolichos green manures
were incorporated.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
6.
Rigon João Paulo Gonsiorkiewicz Calonego Juliano Carlos Capuani Silvia Franzluebbers Alan J. 《Plant and Soil》2021,462(1-2):577-590
Plant and Soil - Cover crop species selection for soybean (Glycine max) production under no-tillage (NT) management may affect soil organic C sequestration by altering the quantity and quality of C... 相似文献
7.
A. J. Franzluebbers 《Plant and Soil》2006,282(1-2):153-164
Tall fescue (Festuca arundinacea Schreb.) is naturally infected with a fungal endophyte, Neotyphodium coenophialum, which produces toxic ergot alkaloids that negatively affect herbivores and that may alter soil organic matter dynamics.
A 60-week mesocosm study with a factorial arrangement of soil type (clay loam and loamy sand) and endophyte infection (with
and without) was conducted to determine potential changes in soil C and N fractions. Forage and root dry-matter production
were greater with than without endophyte infection, while forage C and N concentrations were unaffected. Total, particulate,
mineralizable, and aggregate-associated C and N fractions increased several fold during the course of the experiment due to
large rhizosphere inputs in all treatments. The fraction of total C and N in water-stable macroaggregates (>0.25 mm) was initially
0.43 ± 0.10 and 0.46 ± 0.16, respectively, and increased during the course of the experiment to 0.68 ± 0.06 and 0.56 ± 0.15
when averaged across soil type and endophyte infection level as a result of organic matter cycling and deposition in this
active biophysical fraction. Changes in soil C and N fractions due to endophyte infection were minimal. The lack of detectable
changes in soil C and N fractions due to endophyte infection may have been a result of the overwhelming input of C from roots
and/or the relatively short-term nature (60 weeks). Greater plant productivity of endophyte-infected tall fescue is likely
a contributing mechanism for eventual changes in total and active C and N fractions that have been observed in long-term pastures. 相似文献
8.
In contrast to endophyte-free (E−) tall fescue, endophyte-infected (E+) tall fescue pastures appear to enhance soil carbon sequestration. A hypothetical mechanism that may account for the enhanced carbon sequestration is that the E+ tall fescue affects the soil microbial community or components of it that are involved in organic carbon turnover. A 60-week mesocosm study with a factorial arrangement of soil type, loamy sand (LS) and clay loam (CL), and E+ and E− tall fescue was conducted to determine if the soil microbial communities were affected by the presence of the endophyte. Bulk and rhizosphere soil samples were fixed in paraformaldehyde, and prepared for total direct microbial counts, and with a combination of one of a domain or subdivision fluorescent oligonucleotide probe for enumerating metabolically active Eubacteria, bacterial subdivisions, and Archaea. E+ tall fescue suppressed the archaeal and high G+C gram-positive bacterial communities of the bulk CL, the delta-proteobacterial community in the rhizosphere CL, and the Planctomycetes community of the rhizosphere LS. In the long-term, suppression of these microbial communities may be a factor in enhanced soil carbon sequestration associated with E+ tall fescue. 相似文献
9.
Soil properties and crop performance on a kaolinitic Alfisol after 15 years of fallow and continuous cultivation 总被引:1,自引:1,他引:0
Anthony S. R. Juo Kathrin Franzluebbers Adenike Dabiri Benson Ikhile 《Plant and Soil》1996,180(2):209-217
A long-term field experiment was established on a kaolinitic Alfisol in Ibadan, Nigeria, in 1972. The land was cleared manually from secondary forest and used for (i) continuous no-till cropping with maize (Zea mays L.) and maize/cassava (Manihot esculenta Crantz) intercropping, (ii) planted fallow of guinea grass (Panicum maximum Jacq.), leucaena (Leucaena leucocephala de Wit), and pigeon pea (Cajanus cajan Millsp.), and (iii) natural bush regrowth in a randomized complete block design with three replications. At the end of 15 years, the fallow plots were cleared manually and cropped with maize for three years. The chemical and physical soil properties and crop performance of the newly-cleared plots were compared with those under 15 years of continuous cultivation. A total of 26 woody species were identified on the bush regrowth plots. Above-ground biomass accumulation of the bush plots was 157 Mg ha-1 containing 1316 kg N ha-1. Guinea grass, leucaena and natural bush regrowth plots had comparable organic C concentrations (approximately 20 g kg-1) in the surface soil (0 to 10 cm) after 15 years. The organic C concentration in the surface soil under pigeon pea was the lowest (9.5 g kg-1) among the four fallow treatments. Soil under 15 years of continuous no-till maize with and without residue mulch, respectively, contained approximately half (10 g kg-1) and a quarter (5.7 g kg-1) of the organic C under natural bush or guinea grass fallow. The levels of exchangeable Ca, K, Mg and effective cation exchange capacity (ECEC) were lower in the soils under continuous cultivation than in those under natural bush and planted fallow. Soil acidification occurred in soils under continuous cropping as depicted by the lower pH values and greater exchangeable Al and Mn concentrations compared to the fallow plots. Grain yield of maize (3 to 5 Mg ha-1) without fertilizer application in the plots newly cleared from natural bush, guinea grass and leucaena fallow was comparable with that of continuous no-till maize with residue mulch and chemical fertilizer (N, P, K, Mg, Zn) applications. Among the four fallow treatments, maize grain and stover yields were the lowest in plots cleared from pigeon pea fallow. 相似文献
10.
Increased use of N fertilizer and more intensive cropping due to the rising food demand in the tropics requires design and
evaluation of sustainable cropping systems with minimum soil acidification. The objectives of this study were to quantify
acidification of an Oxic Kandiustalf with different types of N fertilizer in two cropping systems under no-tillage and its
effect on crop performance. Chemical soil properties in continuous maize (Zea mays L.) and maize-cowpea (Vigna unguiculata (L.) Walp) rotation were determined with three N sources (urea (UA), ammonium sulfate (AS) and calcium ammonium nitrate (CAN))
in Nigeria, West Africa, during five years. Chemical soil properties were related to grain yield and diagnostic plant nutrient
concentrations. For the three N sources, the rate of decline in soil pH in maize-cowpea rotation was 57±7.5% of that in continuous
maize, where double the amount of N fertilizer was applied. The rate of soil acidification during the five years was greater
for AS than for UA or CAN in continuous maize, and not different for UA and CAN in both cropping systems. With AS, soil pH
decreased from 5.8 to 4.5 during five years of continuous maize cropping. Exchangeable acidity increased with N fertilization,
but did not reach levels limiting maize or cowpea growth. Return of residues to the soil surface may have reduced soluble
and exchangeable Al levels by providing a source of organic ligands. Soil solution Mn concentrations increased with N fertilization
to levels likely detrimental for crop growth. Symptoms of Mn toxicity were observed on cowpea leaves where AS was applied
to the preceding maize crop, but not on maize plants. Soil acidification caused significant reductions in exchangeable Ca
and effective CEC. Main season maize yield with N fertilization was lower with AS than with UA or CAN, but not different between
UA and CAN during the six years of cropping. The lower maize grain yield with AS than with the other N sources was attributed
to lower pH and a greater extractable Mn concentration with AS. When kaolinitic Alfisols are used for continuous maize cropping,
even under no-tillage with crop residues returned as mulch, the soil may become acidifed to pH values of 5.0 and below after
a few years. The no-till cereal-legume rotation with judicial use of urea or CAN as N sources for the cereal crop is a more
suitable system for these poorly buffered, kaolinitic soils than continuous maize cropping. The use of AS as N source should
be avoided. H Marschner Section editor 相似文献