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Finore Ilaria Kasavi Ceyda Poli Annarita Romano Ida Oner Ebru Toksoy Kirdar Betul Dipasquale Laura Nicolaus Barbara Lama Licia 《World journal of microbiology & biotechnology》2011,27(10):2425-2433
This study reports the purification and biochemical characterization of a raw starch-digesting α-amylase from Geobacillus thermoleovorans subsp. stromboliensis subsp. nov. (strain PizzoT). The molecular weight was estimated to be 58 kDa by SDS–PAGE. The enzyme was highly active over a wide range of pH from
4.0–10.0. The optimum temperature of the enzyme was 70°C. It showed extreme thermostability in the presence of Ca2+, retaining 50% of its initial activity after 90 h at 70°C. The enzyme efficiently hydrolyzed 20% (w/v) of raw starches, concentration
normally used in starch industries. The α-amylase showed an high stability in presence of many organic solvents. In particular
the residual activity was of 73% in presence of 15% (v/v) ethyl alcohol, which corresponds to ethanol yield in yeast fermentation
process. By analyzing its complete amyA gene sequence (1,542 bp), the enzyme was proposed to be a new α-amylase. 相似文献
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Küçükaşik F Kazak H Güney D Finore I Poli A Yenigün O Nicolaus B Oner ET 《Applied microbiology and biotechnology》2011,89(6):1729-1740
Levan is a homopolymer of fructose with many outstanding properties like high solubility in oil and water, strong adhesiveness,
good biocompatibility, and film-forming ability. However, its industrial use has long been hampered by costly production processes
which rely on mesophilic bacteria and plants. Recently, Halomonas sp. AAD6 halophilic bacteria were found to be the only extremophilic species producing levan at high titers in semi-chemical
medium containing sucrose, and in this study, pretreated sugar beet molasses and starch molasses were both found to be feasible
substitutes for sucrose. Five different pretreatment methods and their combinations were applied to both molasses types. Biomass
and levan concentrations reached by the Halomonas sp. AAD6 cells cultivated on 30 g/L of pretreated beet molasses were 6.09 g dry cells/L and 12.4 g/L, respectively. When
compared with literature, Halomonas sp. was found to stand out with its exceptionally high levan production yields on available fructose. Molecular characterization
and monosaccharide composition studies confirmed levan-type fructan structure of the biopolymers. Rheological properties under
different conditions pointed to the typical characteristics of low viscosity and pseudoplastic behaviors of the levan polymers.
Moreover, levan polymer produced from molasses showed high biocompatibility and affinity with both cancerous and non-cancerous
cell lines. 相似文献
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Extracellular polymer substance synthesized by a halophilic bacterium Chromohalobacter canadensis 28
Radchenkova Nadja Boyadzhieva Ivanka Atanasova Nikolina Poli Annarita Finore Ilaria Di Donato Paola Nicolaus Barbara Panchev Ivan Kuncheva Margarita Kambourova Margarita 《Applied microbiology and biotechnology》2018,102(11):4937-4949
Applied Microbiology and Biotechnology - Halophilic microorganisms are producers of a lot of new compounds whose properties suggest promising perspectives for their biotechnological exploration.... 相似文献
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