排序方式: 共有6条查询结果,搜索用时 250 毫秒
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Estaras Matias Ortiz-Placin Candido Castillejo-Rufo Alba Fernandez-Bermejo Miguel Blanco Gerardo Mateos Jose M. Vara Daniel Gonzalez-Cordero Pedro L. Chamizo Sandra Lopez Diego Rojas Adela Jaen Isabel de Armas Noelia Salido Gines M. Iovanna Juan L. Santofimia-Castaño Patricia Gonzalez Antonio 《Journal of physiology and biochemistry》2023,79(1):235-249
Journal of Physiology and Biochemistry - We have investigated the effects of melatonin on major pathways related with cellular proliferation and energetic metabolism in pancreatic stellate cells.... 相似文献
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Matias Estaras Salome Martinez-Morcillo Alfredo García Remigio Martinez Mario Estevez Marcos Perez-Lopez Maria P. Miguez Miguel Fernandez-Bermejo Jose M. Mateos Daniel Vara Gerardo Blanco Diego Lopez Vicente Roncero Gines M. Salido Antonio Gonzalez 《Biology of the cell / under the auspices of the European Cell Biology Organization》2020,112(10):280-299
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Estaras Matias Peña Fernando J. Tapia José A. Fernandez-Bermejo Miguel Mateos Jose M. Vara Daniel Roncero Vicente Blanco Gerardo Lopez Diego Salido Gines M. Gonzalez Antonio 《Journal of physiology and biochemistry》2020,76(2):345-355
Journal of Physiology and Biochemistry - In this study, the effects of melatonin (1 μM–1 mM) on pancreatic stellate cells (PSC) have been examined. Cell viability and... 相似文献
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Molina-Infante J Pazos-Pacheco C Vinagre-Rodriguez G Perez-Gallardo B Dueñas-Sadornil C Hernandez-Alonso M Gonzalez-Garcia G Mateos-Rodriguez JM Fernandez-Bermejo M Gisbert JP 《Helicobacter》2012,17(4):269-276
Background: Using quadruple clarithromycin‐containing regimens for Helicobacter pylori eradication is controversial with high rates of macrolide resistance. Aim: To evaluate antibiotic resistance rates and the efficacy of empirical and tailored nonbismuth quadruple (concomitant) therapy in a setting with cure rates <80% for triple and sequential therapies. Methods: 209 consecutive naive H. pylori‐positive patients without susceptibility testing were empirically treated with 10‐day concomitant therapy (proton pump inhibitors (PPI), amoxicillin 1 g, clarithromycin 500 mg, and metronidazole 500 mg; all drugs b.i.d.). Simultaneously, 89 patients with positive H. pylori culture were randomized to receive triple versus concomitant therapy for clarithromycin‐susceptible H. pylori, and sequential versus concomitant therapy for clarithromycin‐resistant strains. Eradication was confirmed with 13C‐urea breath test or histology 8 weeks after completion of treatment. Results: Per‐protocol (PP) and intention‐to‐treat eradication rates after empirical concomitant therapy without susceptibility testing were 89% (95%CI:84–93%) and 87% (83–92%). Antibiotic resistance rates were: clarithromycin, 20%; metronidazole, 34%; and both clarithromycin and metronidazole, 10%. Regarding clarithromycin‐susceptible H. pylori, concomitant therapy was significantly better than triple therapy by per protocol [92% (82–100%) vs 74% (58–91%), p = 0.05] and by intention to treat [92% (82–100%) vs 70% (57–90%), p = 0.02]. As for antibiotic‐resistant strains, eradication rates for concomitant and sequential therapies were 100% (5/5) vs 75% (3/4), for clarithromycin‐resistant/metronidazole‐susceptible strains and 75% (3/4) vs 60% (3/5) for dual‐resistant strains. Conclusions: Empirical 10‐day concomitant therapy achieves good eradication rates, close to 90%, in settings with multiresistant H. pylori strains. Tailored concomitant therapy is significantly superior to triple therapy for clarithromycin‐susceptible H. pylori and at least as effective as sequential therapy for resistant strains. 相似文献
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Patricia Santofimia-Castaño Deborah Clea Ruy Miguel Fernandez-Bermejo Gines M. Salido Antonio Gonzalez 《Molecular and cellular biochemistry》2014,397(1-2):75-86
Intracellular Ca2+ overload has been considered a common pathological precursor of pancreatic injury. In this study, the effects of melatonin on Ca2+ mobilization induced by cholecystokinin octapeptide (CCK-8) in freshly isolated mouse pancreatic acinar cells have been examined. Changes in intracellular free Ca2+ concentration were followed by single cell fluorimetry. For this purpose, cells were loaded with the Ca2+-sensitive fluorescent dye fura-2-acetoxymethyl ester. In order to evaluate the contribution of Ca2+ transport at the plasma membrane, at the endoplasmic reticulum (ER) or at the mitochondria, cells were incubated with CCK-8 alone or in combination with LaCl3, thapsigargin (Tps), or FCCP to, respectively, uncouple Ca2+ transport at these localizations. The experiments were performed in the absence or in the presence of melatonin in combination with the stimuli mentioned. Our results show that the total Ca2+ mobilization evoked by CCK-8 was attenuated by a 30 % in the presence of 100 µM melatonin compared with the responses induced by CCK-8 alone. Upon inhibition of Ca2+ transport into the ER by Tps, Ca2+ mobilization was also reduced in the presence of melatonin. In the presence of LaCl3 plus melatonin, the total Ca2+ mobilization induced by CCK-8 was significantly decreased, compared with the response obtained without melatonin but in the presence of LaCl3. No major differences were found when the cells were incubated with CCK-8 or Tps alone or in combination with LaCl3 plus melatonin and FCCP, compared with the responses obtained in the absence of FCCP. The initial Ca2+ release from intracellular stores evoked by CCK-8 or Tps was not significantly reduced in the presence of melatonin. The effect of melatonin could be explained on the basis of a stimulated Ca2+ transport out of the cell through the plasma membrane and by a stimulation of Ca2+ reuptake into the ER. Accumulation of Ca2+ into mitochondria might not be a major mechanism stimulated by melatonin. We conclude that melatonin alleviates intracellular Ca2+ accumulation, a situation potentially leading to cell damage in the exocrine pancreas. 相似文献
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