Cytotoxic,clonal fidelity,and antioxidant evaluation of in vitro propagated Vitex negundo var. cannabifolia

Plant Cell, Tissue and Organ Culture (PCTOC) - Vitex negundo L. var. cannabifolia (Sieb. et Zucc.) Hand.-Mazz. is an important medicinal plant rich in bioactive compounds. In vitro tissue culture...     

On Graphical and Numerical Representation of Protein Sequences

Abstract

A new approach using a 3-D Cartesian coordinate system to represent protein sequences has been derived. By the 3-D Graphical representation we make a comparison of sequences belonging to nine different proteins.     

蓖麻毒蛋白A链基因RNAi转化研究

通过基因沉默技术调控蓖麻毒蛋白A链基因的表达,以期获得低毒蓖麻新材料.利用基因克隆技术获得蓖麻毒蛋白A链基因762 bp片段,命名为RTA基因.进一步利用该基因构建了植物RNAi表达载体pBI-RTA-S-AS,通过农杆菌介导法转化蓖麻子叶节,用卡那抗性筛选转化再生植株,PCR进一步鉴定转基因植株.结果表明:克隆得到目的基因长762 bp,与预期结果一致;卡那抗性筛选和PCR鉴定结果显示,获得了3株转基因阳性植株.     

De novo transcriptome analyses reveal multiple metabolic pathways for L-ascorbic acid biosynthesis under drought stress in Pugionium cornutum (L.) Gaertn

Journal of Plant Biochemistry and Biotechnology - Pugionium cornutum (L.) Gaertn., a member of the Brassicaceae family, is a plant that has been used as traditional Chinese medicine and is consumed...     

人胰高血糖素样肽1在转基因番茄中的表达

人胰高血糖素样肽1 (GLP1)是一种短肽激素,对Ⅱ型糖尿病具有很好的疗效.本研究在设计合成GLP1基因并构建植物表达载体的基础上,通过农杆菌介导将GLP1基因导入番茄基因组中,经过PCR扩增和Southern Blot分析,证实GLP1基因已整合进入9个株系的番茄基因组中.Western Blot检测表明,其中4个株系转基因番茄的叶片能够检测到hGLP1融合蛋白的表达.通过Ni-NTA亲和层析分离纯化转基因番茄表达的hGLP1融合蛋白,动物实验表明该融合蛋白具有显著的降血糖生物活性.本研究结果将为转基因番茄作为生物反应器表达药用蛋白提供重要的理论和技术支持,并将为培育具有糖尿病治疗功能的番茄新品种奠定基础.     

Isolation and characterisation of an <Emphasis Type="Italic">HpSHP</Emphasis> gene from <Emphasis Type="Italic">Hosta plantaginea</Emphasis>

Based on genetic and molecular analyses, the ABC model has been proposed to explain the genetic control of floral development. C-class MADS-box genes play crucial roles in Arabidopsis thaliana development by regulating the organ identities of stamens and gynoecium. The present research reports for the first time the cloning of an HpSHP gene from Hosta plantaginea (Lam.) Aschers. Phylogenetic analysis shows that HpSHP is a member of the C-class MADS-box genes that is closely related to C-lineage SHP homologues from monocot species. Semi-quantitative and real-time polymerase chain reaction analyses show that HpSHP expression is stamen and gynoecium specific. HpSHP also has spatial and temporal expression patterns in the reproductive organs of H. plantaginea. A functional analysis is carried out in Arabidopsis by overexpression of HpSHP. Homeotic transformations of sepals into carpelloid organs, bent ovaries, and prematurely shattering fruits are observed in 35S::HpSHP transgenic plants. All these results show that HpSHP plays a crucial role in gynoecium development.     

Local Polynomial Estimation of Heteroscedasticity in a Multivariate Linear Regression Model and Its Applications in Economics

Multivariate local polynomial fitting is applied to the multivariate linear heteroscedastic regression model. Firstly, the local polynomial fitting is applied to estimate heteroscedastic function, then the coefficients of regression model are obtained by using generalized least squares method. One noteworthy feature of our approach is that we avoid the testing for heteroscedasticity by improving the traditional two-stage method. Due to non-parametric technique of local polynomial estimation, it is unnecessary to know the form of heteroscedastic function. Therefore, we can improve the estimation precision, when the heteroscedastic function is unknown. Furthermore, we verify that the regression coefficients is asymptotic normal based on numerical simulations and normal Q-Q plots of residuals. Finally, the simulation results and the local polynomial estimation of real data indicate that our approach is surely effective in finite-sample situations.     

Efficient callus-mediated regeneration and <Emphasis Type="Italic">in vitro</Emphasis> root tuberization in <Emphasis Type="Italic">Trichosanthes kirilowii</Emphasis> Maxim., a medicinal plant

Trichosanthes kirilowii Maxim. is a climbing herb with considerable medicinal value. In this study, efficient protocols for callus-mediated regeneration and in vitro tuberization of this plant were developed. Sterilized stem and leaf tissues were cultured on Murashige and Skoog (MS) medium with plant growth regulators (PGRs), and additives that promoted callus induction and regeneration. Both stem and leaf tissues showed the best response (100%) for callus initiation on MS medium supplemented with 4.5-μM 2,4-dichlorophenoxyacetic acid (2,4-D). Efficient shoot organogenesis was obtained by exposing the callus tissue to 4.6-μM kinetin, 2.2-μM 6-benzylaminopurine, and 2.7-μM 1-naphthylacetic acid (NAA) along with 12.6-μM copper sulfate, which yielded a shoot regeneration rate of 85.5% and 28 shoots derived from each callus. In vitro shoots were best rooted on half-strength (1/2) MS medium with 2.7-μM NAA. Tuberous roots were efficiently induced on rooting medium with 5% (w/v) sucrose under short illumination conditions (8 h photoperiod). Rooted plantlets were successfully acclimatized in pots with a >?90% survival rate. This protocol provides an effective method for callus-mediated regeneration and in vitro root tuberization.     

Season- and age-associated telomerase activity in <Emphasis Type="Italic">Ginkgo biloba</Emphasis> L.

Telomeres have lately received considerable attention in the development of broad-leaved tree species. In order to determine tissue-, sex-, season- and age-specific changes in telomerase activity in ginkgo trees, analyses of the telomerase repeat amplification protocol were carried out. In all of the tissues detected (embryonal callus, microspore tissues and leaves) telomerase activity was found, with differences between these activities statistically significant (P < 0.05). The highest telomerase activity was found in embryonal callus, suggesting that ginkgo trees have tissue-specific telomerase activity. Tissues containing high levels of dividing cells also have high levels of telomerase activity. No significant difference of telomerase activity was found between male and female trees (P > 0.05). In the annual development cycle, the highest telomerase activity was found in April and a decreasing trend over time in the four age groups studied: 10, 20, 70 and 700 year. The most obvious decline appeared in trees of the 700 year old group, suggesting that ginkgo trees have season-specific telomerase activities and trees of various ages react differently to seasonal changes. The mean annual telomerase activity showed a regular decreasing trend in all leaf samples analyzed from 10 to 700 year old ginkgo trees. We conclude that maintenance of telomere length depends on season- and age- associated telomerase activity. An optimal telomere length is regulated and maintained by telomerase in Ginkgo biloba L.