全文获取类型
收费全文 | 209篇 |
免费 | 17篇 |
出版年
2021年 | 4篇 |
2020年 | 1篇 |
2019年 | 4篇 |
2018年 | 5篇 |
2016年 | 3篇 |
2015年 | 4篇 |
2014年 | 11篇 |
2013年 | 27篇 |
2012年 | 14篇 |
2011年 | 9篇 |
2010年 | 4篇 |
2009年 | 6篇 |
2008年 | 10篇 |
2007年 | 14篇 |
2006年 | 10篇 |
2005年 | 10篇 |
2004年 | 11篇 |
2003年 | 14篇 |
2002年 | 9篇 |
2001年 | 11篇 |
2000年 | 6篇 |
1999年 | 4篇 |
1998年 | 2篇 |
1997年 | 2篇 |
1996年 | 1篇 |
1995年 | 2篇 |
1994年 | 1篇 |
1993年 | 1篇 |
1992年 | 7篇 |
1991年 | 1篇 |
1990年 | 1篇 |
1989年 | 3篇 |
1988年 | 3篇 |
1985年 | 1篇 |
1984年 | 3篇 |
1982年 | 1篇 |
1980年 | 2篇 |
1978年 | 1篇 |
1975年 | 1篇 |
1974年 | 1篇 |
1972年 | 1篇 |
排序方式: 共有226条查询结果,搜索用时 234 毫秒
1.
D J Montell 《Development (Cambridge, England)》1999,126(14):3035-3046
Cell migrations are found throughout the animal kingdom and are among the most dramatic and complex of cellular behaviors. Historically, the mechanics of cell migration have been studied primarily in vitro, where cells can be readily viewed and manipulated. However, genetic approaches in relatively simple model organisms are yielding additional insights into the molecular mechanisms underlying cell movements and their regulation during development. This review will focus on these simple model systems where we understand some of the signaling and receptor molecules that stimulate and guide cell movements. The chemotactic guidance factor encoded by the Caenorhabditis elegans unc-6 locus, whose mammalian homolog is Netrin, is perhaps the best known of the cell migration guidance factors. In addition, receptor tyrosine kinases (RTKs), and FGF receptors in particular, have emerged as key mediators of cell migration in vivo, confirming the importance of molecules that were initially identified and studied in cell culture. Somewhat surprisingly, screens for mutations that affect primordial germ cell migration in Drosophila have revealed that enzymes involved in lipid metabolism play a role in guiding cell migration in vivo, possibly by producing and/or degrading lipid chemoattractants or chemorepellents. Cell adhesion molecules, such as integrins, have been extensively characterized with respect to their contribution to cell migration in vitro and genetic evidence now supports a role for these receptors in certain instances in vivo as well. The role for non-muscle myosin in cell motility was controversial, but has now been demonstrated genetically, at least in some cell types. Currently the best characterized link between membrane receptor signaling and regulation of the actin cytoskeleton is that provided by the Rho family of small GTPases. Members of this family are clearly essential for the migrations of some cells; however, key questions remain concerning how chemoattractant and chemorepellent signals are integrated within the cell and transduced to the cytoskeleton to produce directed cell migration. New types of genetic screens promise to fill in some of these gaps in the near future. 相似文献
2.
Summary We study the leg morphology and feeding postures of two subspecies of the Blue Tit (Parus caeruleus; Tenerife island and the Iberian Peninsula) and the Coal Tit (Parus ater; Iberian Peninsula). We search for evidence supporting the hypothesis of convergent evolution in morphological and ecological traits and we discuss the role of ecomorphological hypotheses as predictors of foraging differences at the intraspecific level. To overcome the problems introduced by environmental characteristics not related to locomotion and competition, we make observations under controlled situations to manage food quality and food access. We determine that the island Blue Tit has a longer tarsometatarsus, larger foot span and a more proximal insertion of the tibialis cranialis muscle (flexor of the tarsometatarsus) than the mainland Blue Tit. These morphological differences are consistent with the more frequent use of hanging and clinging head-up postures by the Iberian Blue Tit. Several ecomorphological hypotheses obtained at the interspecific level with other taxa, have proved to be of high predictive value for explaining ecological differences considering morphological evolution. The Tenerife Blue Tit and the Iberian Coal Tit clearly show close convergence in both feeding postures and leg structure, although some differences in morphology were found between these two species. Convergence in foraging methods between the island Blue Tit and the mainland Coal Tit can be explained without considering current interspecific competition as a determinant of niche space. 相似文献
3.
Esterina Pascale Christine Liu Eulalia Valle Karen Usdin Anthony V. Furano 《Journal of molecular evolution》1993,36(1):9-20
Summary All modern mammals contain a distinctive, highly repeated (⩾50,000 members) family of long interspersed repeated DNA called
the L1 (LINE 1) family. While the modern L1 families were derived from a common ancestor that predated the mammalian radiation
∼80 million years ago, most of the members of these families were generated within the last 5 million years. However, recently
we demonstrated that modern murine (Old World rats and mice) genomes share an older long interspersed repeated DNA family
that we called Lx. Here we report our analysis of the DNA sequence of Lx family members and the relationship of this family
to the modern L1 families in mouse and rat. The extent of DNA sequence divergence between Lx members indicates that the Lx
amplification occurred about 12 million years ago, around the time of the murine radiation. Parsimony analysis revealed that
Lx elements were ancestral to both the modern rat and mouse L1 families. However, we found that few if any of the evolutionary
intermediates between the Lx and the modern L1 families were extensively amplified. Because the modern L1 families have evolved
under selective pressure, the evolutionary intermediates must have been capable of replication. Therefore, replicationcompetent
L1 elements can reside in genomes without undergoing extensive amplification. We discuss the bearing of our findings on the
evolution of L1 DNA elements and the mammalian genome. 相似文献
4.
Complete transformation by adenovirus 2 requires both E1A proteins 总被引:45,自引:0,他引:45
5.
Elimination of mRNA splicing by a point mutation outside the conserved GU at 5'' splice sites 总被引:9,自引:4,他引:5 下载免费PDF全文
Nearly all mRNA introns begin with the dinucleotide GU. Mutations in either of these virtually invariant bases have been found to inactivate the corresponding 5' splice site. Until now single base changes in neighboring bases have not been found to completely inactivate a 5' splice site. Here we show that a single A----U transversion in the third position of the adenovirus 2 E1A 13S mRNA intron does prevent RNA splicing at the corresponding 5' splice site. 相似文献
6.
Differential localizations of and requirements for the two Drosophila ninaC kinase/myosins in photoreceptor cells 下载免费PDF全文
The ninaC gene encodes two retinal specific proteins (p132 and p174) consisting of a protein kinase domain joined to a domain homologous to the head region of the myosin heavy chain. The putative myosin domain of p174 is linked at the COOH-terminus to a tail which has some similarities to myosin-I tails. In the current report, we demonstrate that the ninaC mutation results in light- and age-dependent retinal degeneration. We also show that ninaC flies display an electrophysiological phenotype before any discernible retinal degeneration indicating that the electrophysiological defect is the primary effect of the mutation. This suggests that ninaC has a role in phototransduction and that the retinal degeneration is a secondary effect resulting from the defect in phototransduction. To examine the requirements for the individual ninaC isoforms, mutant alleles were generated which express only p132 or p174. Elimination of p174 resulted in a ninaC phenotype as strong as the null allele; however, elimination of p132 had little if any effect. As a first step in investigating the basis for the difference in requirements for p174 and p132 we performed immuno-localization at the electron microscopic level and found that the two isoforms display different subcellular distributions in the photoreceptor cells. The p132 protein is restricted primarily to the cytoplasm and p174 to the rhabdomeres, the microvillar structure which is the site of action of many of the steps in phototransduction. This suggests that the p174 myosin-I type tail is the domain responsible for association with the rhabdomeres and that the substrate for the p174 putative kinase may be a rhabdomeric protein important in photo-transduction. 相似文献
7.
Genomic clones containing the full coding sequences of the two subunits of the Ca2+/calmodulin-stimulated protein phosphatase, calcineurin, were isolated from a Drosophila melanogaster genomic library using highly conserved human cDNA probes. Three clones encoded a 19.3-kDa protein whose sequence is 88% identical to that of human calcineurin B, the Ca(2+)-binding regulatory subunit of calcineurin. The coding sequences of the Drosophila and human calcineurin B genes are 69% identical. Drosophila calcineurin B is the product of a single intron-less gene located at position 4F on the X chromosome. Drosophila genomic clones encoding a highly conserved region of calcineurin A, the catalytic subunit of calcineurin, were used to locate the calcineurin A gene at position 21 EF on the second chromosome of Drosophila and to isolate calcineurin A cDNA clones from a Drosophila embryonic cDNA library. The structure of the calcineurin A gene was determined by comparison of the genomic and cDNA sequences. Twelve exons, spread over a total of 6.6 kilobases, were found to encode a 64.6-kDa protein 73% identical to either human calcineurin A alpha or beta. At the nucleotide level Drosophila calcineurin A cDNA is 67 and 65% identical to human calcineurin A alpha and beta cDNAs, respectively. Major differences between human and Drosophila calcineurins A are restricted to the amino and carboxyl termini, including two stretches of repetitive sequences in the carboxyl-terminal third of the Drosophila molecule. Motifs characteristic of the putative catalytic centers of protein phosphatase-1 and -2A and calcineurin are almost perfectly conserved. The calmodulin-binding and auto-inhibitory domains, characteristic of all mammalian calcineurins A, are also conserved. A remarkable feature of the calcineurin A gene is the location of the intron/exon junctions at the boundaries of the functional domains and the apparent conservation of the intron/exon junctions from Drosophila to man. 相似文献
8.
9.
Multiple classes of cell surface receptors and ion channels participate in the detection of changes in environmental stimuli, and thereby influence animal behavior. Among the many classes of ion channels, Transient Receptor Potential (TRP) cation channels are notable in contributing to virtually every sensory modality, and in controlling a daunting array of behaviors. TRP channels appear to be conserved in all metazoan organisms including worms, insects and humans. Flies encode 13 TRPs, most of which are expressed and function in sensory neurons, and impact behaviors ranging from phototaxis to thermotaxis, gravitaxis, the avoidance of noxious tastants and smells and proprioception. Multiple diseases result from defects in TRPs, and flies provide an excellent animal model for dissecting the mechanisms underlying “TRPopathies.” Drosophila TRPs also function in the sensation of botanically derived insect repellents, and related TRPs in insect pests are potential targets for the development of improved repellents to combat insect-borne diseases. 相似文献
10.