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Relationships among the streptothricin resistance transposons Tn1825 and Tn1826 and the trimethoprim resistance transposon Tn7 总被引:9,自引:0,他引:9
The streptothricin resistance transposons Tn1825 and Tn1826 are closely related, based on physical and genetic characteristics, to the trimethoprim resistance transposon Tn7. These transposons may be considered to be members of a transposon family sharing in common the transposition functions and a basic streptomycin/spectinomycin resistance determinant but differing from one another with respect to particular additional resistance genes inserted to the left of the aadA gene. 相似文献
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Schubert Hendrik; Schiewer Ulrich; Tschirner Erhard 《Journal of plankton research》1989,11(2):353-359
The fluorescence characteristics of the cyanobacteria Synechocystisaquatilis Sauv., Microcystis firma (Breb. et Lenorm.) Schmidleand Synechococcus leopoliensis (Racib.) Kom. and the green algaScenedesmus quadricauda (Turp.) Breb. were examined. In thethree cyanobacteria, phycocyanin is the main accessory pigment.Phycoerythrin is not present in our investigated strains ofcyanobacteria. The highest excitation of the chlorophyll a (Chla) fluorescence of cyanobacteria resulted from light with wavelengthsof 620630 nm. A definite Kautsky effectis also evident at this wavelength. However, excitation withblue light (420520 nm) produced only very slight fluorescence.The Kautsky effect is not evident at these wavelengths, evenat high photon flux densities. For Scenedesmus, fluorescencecharacteristics typical of green algae were found. The fluorescenceexcitation of cyanobacteria at 620 nm corresponds to a photosynthesispeak in the action spectrum measured in terms of O2 production.The results underline the necessity of fluorescence measurementsat several wavelengths whenever mixed populations are involved.Such measurements also present possibilities for more accurateestimation of biomass and potential photosynthetic productionin mixed populations. 相似文献
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Freeze etching studies in a symbiotic and a freeliving strain of Chroococcidiopsis revealed a specific layer in the outer cell wall not described so far from Cyanophyta. The layer showed a complex organisation: The main unit are ribbons, 2–3 nm thick, striated at right angle to the longitudinal axis. They are interwoven to a patchwork-like leaflet. The ribbons are virtually composed of globular particles associated in parallel rows. The cytoplasmic membrane and the cell walls of the symbiotic and the free-living strain were compared.Abbreviations cm
cytoplasmic membrane
- CW 1,2,3
cell wall layer 1,2,3
- EF
exoplasmic fracture face
- PF
protoplasmic fracture face 相似文献
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Rolf Erhard 《Development genes and evolution》1959,151(4):381-429
Ohne ZusammenfassungDie Arbeit wurde auf Anregung von Herrn Prof. Dr. H.Wurmbach ausgeführt. Ich danke ihm für die freundliche Förderung der Arbeit, sowie Herrn Prof. Dr. A.Goebel, Pathol. Inst. d. Univ. Köln, für wertvolle Hinweise. Die Untersuchungen wurden von derDeutschen Forschungsgemeinschaft, Bad Godesberg, gefördert. 相似文献
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Martin Haardt Bettina Kempf Elke Faatz Erhard Bremer 《Molecular genetics and genomics : MGG》1995,246(6):783-796
The ProP and ProU transport systems of Escherichia coli mediate the uptake of several osmoprotectants including glycine betaine. Here we report that both ProP and ProU are involved in the transport of the potent osmoprotectant proline betaine. A set of isogenic E. coli strains carrying deletions in either the proP or proU loci was constructed. The growth properties of these mutants in high osmolarity minimal media containing 1 mM proline betaine demonstrated that the osmoprotective effect of this compound was dependent on either an intact ProP or ProU uptake system. Proline betaine competes with glycine betaine for binding to the proU-encoded periplasmic substrate binding protein (ProX) and we estimate a KD of 5.2 μM for proline betaine binding. This value is similar to the binding constant of the ProX protein determined previously for the binding of glycine betaine (KD of 1.4 μM). Our results thus demonstrate that the binding-protein-dependent ProU transport system of E. coli mediates the efficient uptake of the osmoprotectants glycine betaine and proline betaine. 相似文献