全文获取类型
收费全文 | 566篇 |
免费 | 55篇 |
出版年
2021年 | 4篇 |
2020年 | 7篇 |
2019年 | 9篇 |
2018年 | 5篇 |
2017年 | 5篇 |
2016年 | 13篇 |
2015年 | 26篇 |
2014年 | 15篇 |
2013年 | 17篇 |
2012年 | 18篇 |
2011年 | 30篇 |
2010年 | 24篇 |
2009年 | 17篇 |
2008年 | 22篇 |
2007年 | 26篇 |
2006年 | 23篇 |
2005年 | 22篇 |
2004年 | 29篇 |
2003年 | 9篇 |
2002年 | 25篇 |
2001年 | 18篇 |
2000年 | 27篇 |
1999年 | 10篇 |
1998年 | 12篇 |
1997年 | 12篇 |
1996年 | 8篇 |
1995年 | 7篇 |
1994年 | 7篇 |
1993年 | 10篇 |
1992年 | 15篇 |
1991年 | 18篇 |
1990年 | 12篇 |
1989年 | 16篇 |
1988年 | 9篇 |
1987年 | 9篇 |
1986年 | 5篇 |
1985年 | 7篇 |
1984年 | 6篇 |
1983年 | 6篇 |
1982年 | 3篇 |
1981年 | 4篇 |
1980年 | 4篇 |
1979年 | 6篇 |
1978年 | 5篇 |
1977年 | 3篇 |
1975年 | 5篇 |
1974年 | 4篇 |
1973年 | 3篇 |
1971年 | 5篇 |
1970年 | 3篇 |
排序方式: 共有621条查询结果,搜索用时 46 毫秒
1.
2.
Post-natal myogenic satellite cells, isolated from the sternomandibularis muscles of bovine at slaughter were used for primary culture studies. Isolated satellite cells tended to differentiate into multinucleated myotubes more efficiently if initially plated on to a fibronectin substratum. Bovine-derived satellite cells displayed greater fused cell numbers when exposed to Dulbecco's Modified Eagle's Medium (DMEM) supplemented with horse serum than similar supplementation with fetal calf serum (P less than 0.05) or sheep serum (P less than 0.05). In addition, differentiation appeared nearly complete after 4 days exposure to DMEM-1% horse serum as verified by beta-D-arabinofuranosyl-cytosine addition to cultures. Collectively, these data provide the first evidence that satellite cells can be isolated from a bovine skeletal muscle. Furthermore, these data indicate that bovine-derived satellite cells can be induced to undergo substantial morphological differentiation in vitro. 相似文献
3.
Julian J. Dodson 《Environmental Biology of Fishes》1988,23(3):161-182
Synopsis Fish migration may be viewed as the product of two processes; the selection and tracking of optimal environmental conditions
through time and space, and the use of predictive information about environmental structure to bias movements towards a goal.
The establishment and maintenance of directional bias is based on the interaction of experience and instinct. The preoccupation
of much fish orientation research with innate fixed patterns of behavior on one hand and hydrodynamics on the other has led
us to underestimate the possibility that orientation is a flexible process relying on developmental sequences, calibration
of the motor-sensory interaction based on experience and the learning of environmental pattern. Evidence illustrating how
experience and learning may influence the direction of movement and how the goal is recognized is presented according to two
general categories: (a) imprinting and early experience and (b), spatial learning, including the social transmission of migratory
routes and directions. In the first category, the olfactory hypothesis of salmon homing is briefly reviewed and new data presented
describing olfactory imprinting in Atlantic salmon,Salmo salar. In the second category, evidence is presented demonstrating the modifiability of sun-compass orientation and the ability
of some fish species to learn the spatial distribution of landmarks. The role of social transmission in the migration of coral
reef fishes is reviewed. The possible role of these learning phenomena in the formation of familiar area maps, route-based
and location-based navigation and the critical distance factor is considered. The relationship between life history and the
nature of learning in migratory orientation is discussed 相似文献
4.
To define gonadotrophin secretion rates in the prepubertal heifer, 12 Hereford x Friesian heifers were blood-sampled at 15-min intervals for periods of 24 h every 4 weeks from 3 weeks of age until puberty. Radioimmunoassay of plasma LH concentrations showed that, although LH episode frequency increased with age, overall mean LH concentrations and basal LH concentrations decreased between 3 and 15 weeks of age and then increased to 35 weeks of age. The validity of these trends in relation to biological activity of plasma LH was investigated using an in-vitro Leydig cell bioassay. Samples were selected from 24-h profile bleeds of 4 heifers at 3, 7, 11, 15, 19, 27 and 39 weeks of age. No significant differences were found in the patterns of change in overall mean LH concentrations, basal LH concentrations or LH episode amplitude when comparing the estimates obtained by radioimmunoassay with those by bioassay from birth over the prepubertal period. These results indicate that the changes with age observed by radioimmunoassay are representative of changes in biologically active hormone. 相似文献
5.
The time elvers of the American eel, Anguilla rostrata , spend in an estuary prior to their migration into fresh water was assessed. A distinct mark was formed on elvers' otoliths during their first 2 to 3 weeks in the river estuary. This mark was used to distinguish between growth in fresh water and in salt water. Migrating eels collected at a falls 4 km from the estuary exhibited bimodal length and weight distributions. Frequency distributions showed that eels collected in the estuary were smaller and had smaller otoliths than eels collected at the falls, indicating that elvers do not reach the falls in the same year as they enter the estuary. The three modal groups most probably represent three age–classes. However, the otoliths of elvers collected in the estuary had only the mark of transition whereas eels in the first and second mode at the falls usually had two rings (1–4) and four rings (3–6) per otolith, respectively, in addition to the mark of transition, as viewed under SEM. The possibility that ring formation is not annual means that the use of otoliths for the age determination of eels in this study has not been validated. 相似文献
6.
The in vitro activity of several new imidazoles, cloconazole, sulconazole, butoconazole, isoconazole and fenticonazole, were compared with those of amphothericin B, flucytosine, and three azoles: econazole, miconazole and ketoconazole against isolates of pathogenic Candida. A total of 186 clinical isolates of 10 species of the genus Candida and two culture collection strains were tested by an agar-dilution technique. Isoconazole was the most active azole, followed by butoconazole and sulconazole. Differences between some of the species in their susceptibility to the antifungal agents were noted. Sulconazole and cloconazole had the highest activity in vitro against 106 isolates of C. albicans. Butoconazole and isoconazole were also very active against isolates of C. albicans, and were the most active azole compounds against 80 isolates of Candida spp. 相似文献
7.
Insulin's structural behavior and its relation to activity 总被引:3,自引:0,他引:3
This paper discusses the hypothesis that insulin undergoes a conformational change either before or during its binding to the receptor. The evidence for this is not conclusive but allows us to reconcile the following observations: (1) no chemical modification or deletion of invariant surface residues has abolished the hormone's activity—only reduced its potency. (2) Reduction in potency follows many modifications to different side chains, both variant and invariant. (3) There are insulins with perfectly preserved structure (by the criteria of aggregation, spectroscopy, and x-ray analysis) that have markedly reduced potency. (4) Insulins with disturbed structure still exhibit real, sometimes substantial activity. 相似文献
8.
Sediments of the Upper Cretaceous (Campanian) Oldman Formation at Dinosaur Provincial Park, Alberta, have yielded an exceedingly rich fauna of dinosaurs. These fluviatile sediments show characteristics of both meandering and braided channels. Fossil molluscs, plants, and diverse salamanders indicate that Oldman sediments were deposited in fresh water, and the very sparse agglutinated Foraminifera recovered fail to controvert this conclusion. Annual growth rings in wood and vertebrate of Champsosaurus demonstrate that the climate was seasonal, and current botanic interpretations suggest that the climate was an equable, warm temperate one. Dinosaurian remains represent all stages of disarticulation from complete skeletons to isolated bones. They are common in channel sediments and rare in overbank deposits, suggesting that the animals preserved died in the water of channels. The persistence of the characteristics sedimentary association of fossils over a 200 ft. stratigraphic interval demonstrates that regular, not catastrophic events determined the preservation of individuals. Stages of progressive decomposition of dinosaurs are inferred from the condition of fossils collected from the Park. Disarticulated small bones occur at interfaces between mudstone and sandstone in which the sandstone lies above the mudstone, and also in intraformational conglomerates. Ceratopsians are identified as dwellers of the swampy lowlands along with hadrosaurs. Hadrosaurs, ceratopsians, and possibly even carnosaurs spent significant portions of their daily lives in water. These dinosaurs did not breed in “uplands”, but possibly in dry areas lateral to the streams. 相似文献
9.
A novel experimental method was developed which allows the determination of the threshold concentration of sucrose by use of a linear sucrose gradient in water. With this method a continuous tasting of the test-liquid is possible. A panel of 15 persons experienced in taste-testing was used. Three gradients of different steepness were applied: 0 to 1.5% (w/w) sucrose in 2 min (I), 3 min (II) and 4 min (III). The results of the new method were compared with those of the standard method (DIN). With gradients I and II we found values which were significantly higher than those of the standard method (I: 0.49% (w/w); II: 0.46% (w/w); DIN: 0.31% (w/w)), whereas with gradient III the same threshold value was found as with the DIN-Method (III: 0.32% (w/w)). 相似文献
10.
P. M. Dodson M. Beevers R. Hallworth M. J. Webberley R. F. Fletcher K. G. Taylor 《BMJ (Clinical research ed.)》1989,298(6668):227-230
OBJECTIVE--To determine the effect of moderate dietary sodium restriction on the hypertension of non-insulin-dependent (type II) diabetes. DESIGN--Randomised parallel controlled study of moderate sodium restriction for three months compared with usual diabetic diet, followed by randomised double blind crossover trial of sustained release preparation of sodium for one month versus placebo for one month in patients continuing with sodium restriction. SETTING--Patients attending diabetic outpatient clinic of city hospital. PATIENTS--Thirty four patients with established type II diabetes complicated by mild hypertension (systolic blood pressure greater than 160 mm Hg or diastolic pressure greater than 95 mm Hg on three consecutive occasions). Patients already taking antihypertensive agents (but not diuretics) not barred from study provided that criteria for mild hypertension still met. Conditions precluding patients from study were diabetic or hypertensive nephropathy, cardiac failure, and pregnancy. INTERVENTIONS--After run in phase with recordings at seven weeks, three weeks, and time zero patients were allocated at random to receive moderate dietary sodium restriction for three months (n = 17) or to continue with usual diabetic diet. Subsequently nine patients in sodium restriction group continued with regimen for a further two months, during which they completed a randomised double blind crossover trial of sustained release preparation of sodium (Slow Sodium 80 mmol daily) for one month versus matching placebo for one month. END POINT--Reduction in blood pressure in type II diabetics with mild hypertension. MEASUREMENTS AND MAIN RESULTS--Supine and erect blood pressure, body weight, and 24 hour urinary sodium and potassium excretion measured monthly during parallel group and double blind crossover studies. After parallel group study sodium restriction group showed significant reduction in systolic blood pressure (supine 19.2 mm Hg, erect 21.4 mm Hg; p less than 0.001) and mean daily urinary sodium excretion (mean reduction 60 mmol/24 h). There were no appreciable changes in weight, diabetic control, or diastolic pressure. No significant changes occurred in controls. In double blind crossover study mean supine systolic blood pressure rose significantly (p less than 0.005) during sodium supplementation (to 171 mm Hg) compared with value after three months of sodium restriction alone (159.9 mm Hg) and after one month of placebo (161.8 mm Hg). CONCLUSIONS--Moderate dietary restriction of sodium has a definite hypotensive effect, which may be useful in mild hypertension of type II diabetes. 相似文献