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1.
It has been established that in healthy persons functional brain asymmetry positively influences the effective performance of sensomotor function of oral cavity organs. Individual brain asymmetry is of considerable importance, the higher it is, the quicker the final results of sensomotor function are achieved. The character of brain asymmetry (right hand or left hand) does not practically influence the performance of sensomotor functions. 相似文献
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Lesia Dmytrenko Michal Cicanic Miroslava Anderova Ivan Vorisek Ole Petter Ottersen Eva Sykova Lydia Vargova 《PloS one》2013,8(7)
Aquaporin-4 (AQP4) is the primary cellular water channel in the brain and is abundantly expressed by astrocytes along the blood-brain barrier and brain-cerebrospinal fluid interfaces. Water transport via AQP4 contributes to the activity-dependent volume changes of the extracellular space (ECS), which affect extracellular solute concentrations and neuronal excitability. AQP4 is anchored by α-syntrophin (α-syn), the deletion of which leads to reduced AQP4 levels in perivascular and subpial membranes. We used the real-time iontophoretic method and/or diffusion-weighted magnetic resonance imaging to clarify the impact of α-syn deletion on astrocyte morphology and changes in extracellular diffusion associated with cell swelling in vitro and in vivo. In mice lacking α-syn, we found higher resting values of the apparent diffusion coefficient of water (ADCW) and the extracellular volume fraction (α). No significant differences in tortuosity (λ) or non-specific uptake (k′), were found between α-syn-negative (α-syn −/−) and α-syn-positive (α-syn +/+) mice. The deletion of α-syn resulted in a significantly smaller relative decrease in α observed during elevated K+ (10 mM) and severe hypotonic stress (−100 mOsmol/l), but not during mild hypotonic stress (−50 mOsmol/l). After the induction of terminal ischemia/anoxia, the final values of ADCW as well as of the ECS volume fraction α indicate milder cell swelling in α-syn −/− in comparison with α-syn +/+ mice. Shortly after terminal ischemia/anoxia induction, the onset of a steep rise in the extracellular potassium concentration and an increase in λ was faster in α-syn −/− mice, but the final values did not differ between α-syn −/− and α-syn +/+ mice. This study reveals that water transport through AQP4 channels enhances and accelerates astrocyte swelling. The substantially altered ECS diffusion parameters will likely affect the movement of neuroactive substances and/or trophic factors, which in turn may modulate the extent of tissue damage and/or drug distribution. 相似文献
3.
Hryshchenko NV Bychkova AM Pichkur NA Skyban HV Dmytrenko VV Livshyts' LA 《T?Sitologii?a i genetika》2003,37(6):55-59
Charcot-Marie-Tooth neuropathy (CMT) is one of the most common hereditary disorders, affecting 1:2500 individuals. CMT is a heterogeneous group of disorders characterized by chronic peripheral motor and sensory neuropathy. We have performed the detection of 1.5 Mb CMT1A tandem duplication in 17p11.2-12 chromosome region for autosome-dominant CMT1 patients and their relatives using the analysis of two (CA)n polymorphic microsatellite loci: 17S921 and 17S1358 localised in the duplication region. CMT1A duplication was found in three of five autosome-dominant CMT1 families. It has been shown that CMT1A duplication analysis is important for early differential diagnosis of CMT including prenatal diagnosis and genetic consulting in high risk families. 相似文献
4.
L. N. Shapoval O. V. Dmytrenko L. S. Pobegailo L. G. Stepanenko V. F. Sagach 《Neurophysiology》2007,39(3):201-212
In acute experiments on normotensive rats and those with genetically determined hypertension (urethane anesthesia), we studied
hemodynamic effects resulting from modulation of the activities of neuronal NO synthase (NOS-1), arginase II, and superoxide
dismutase, and also of the mitochondrial permeability in medullary cardiovascular neurons. Unilateral microinjections of either
a nitric oxide (NO) donor, sodium nitroprusside, or a substrate for endogenous NO synthesis, L-arginine, into the medullary
cardiovascular nuclei (nucl. tractus solitarius, NTS, nucl. ambiguous, AMB, paramedian nucleus, PMn, and lateral reticular nucleus LRN) were shown to induce hemodynamic responses with rather similar dynamics in both normotensive and spontaneously hypertensive
rats, although in the latter the reactions were more intense. Injections of an antagonist of NOS-1, NG nitro-L-arginine (L-NNA), into the medullary nuclei under study in spontaneously hypertensive rats resulted in shifts of
the systemic arterial pressure (SAP), which did not differ dramatically from those observed in normotensive animals. The data
obtained serve as the background for the suggestion that the functional activity of NOS-1 is not fundamentally impaired under
hypertension conditions, but, probably, the amount of the substrate for adequate synthesis of NO via the NO-synthase pathway
of metabolism of L-arginine is insufficient. Considering this, we examined the functional activity of arginase, an enzyme
that also, similarly to NOS, uses L-arginine for metabolic transformation. Injections of antagonists of arginase, norvaline
or α-difluoromethylornithine hydrochloride (DFMO), into populations of the medullary neurons under study induced similar shifts
of the SAP in normotensive and spontaneously hypertensive rats, and those responses did not differ significantly from the
effects of inhibition of the NOS-1 activity. Thus, both the above-mentioned enzymes are potentially active in normotensive
and spontaneously hypertensive rats; so, a possibility for their competition for L-arginine in certain situations does exist.
Modulation of the mitochondrial permeability in medullary cardiovascular neurons in normotensive and spontaneously hypertensive
rats induced significant hemodynamic effects. In particular, an increase in the mitochondrial permeability in the medullary
cardiovascular nuclei by injections of an inductor of mitochondrial permeability transition pore (mPTP) opening, phenylarsine
oxide (PAO), was accompanied by SAP drops in both normotensive and spontaneously hypertensive rats; the effects were dose-dependent
and, in some cases, irreversible. A decrease in the mitochondrial permeability in the neurons under study by injections of
an inhibitor of mPTP, melatonin, induced mostly hypertensive responses, although in some experiments we observed hypotensive
and two-phase responses.
Neirofiziologiya/Neurophysiology, Vol. 39, No. 3, pp. 232–244, May–June, 2007. 相似文献
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A method for endogenous formaldehyde (FA) level evaluation has been worked out. The method involves the administration of dimedone, which forms the stable complex with FA, and the determination of formaldimedone concentration in biological samples by the fluorescence approach. The method was tested on rat's models of FA metabolism modulation. Animals received FA (10 mg/kg); or methylamine - substrate of FA-generating enzyme SSAO, (250 mg/kg); or semicarbazide - SSAO inhibitor, (200 mg/kg). Concentration of FA bound with dimedone in the liver tissue were, correspondingly: 7.5 +/- 1.5 mkg/kg; 5.4 +/- 0.9 mkg/kg; 2.4 +/- 0.7 mkg/kg; control - 4.2 +/- 1.4 mkg/kg. Obtained data indicate, that the elaborated method gives reliable information about FA level. 相似文献
7.
Aruna Kasoju M Lakshmi Narasu Charuvaka Muvva Bathula VV SubbaRao 《Bioinformation》2012,8(14):684-686
Aflatoxins are polyketide-derived secondary metabolites produced by Aspergillus spp. The toxic effects of aflatoxins have adverse
consequences for human health and agricultural economics. The aflR gene, a regulatory gene for aflatoxin biosynthesis, encodes a
protein containing a zinc-finger DNA-binding motif. AFLR-Protein three-dimensional model was generated using Robetta server.
The modeled AFLR-Protein was further optimization and validation using Rampage. In the simulations, we monitored the
backbone atoms and the C-α-helix of the modeled protein. The low RMSD and the simulation time indicate that, as expected, the
3D structural model of AFLR-protein represents a stable folding conformation. This study paves the way for generating computer
molecular models for proteins whose crystal structures are not available and which would aid in detailed molecular mechanism of
inhibition of aflatoxin. 相似文献
8.
Dmytrenko MP Shandrenko SH Petrun' LM Kishko TO Sylonova NV Latyshko NV Hudkova OO Sushkova VV 《Ukrainski? biokhimicheski? zhurnal》2010,82(4):86-91
Subchronic administration of semicarbazide in the experiment with the rats was used to reduce the formaldehyde level in the organism in order to reveal the interaction between formaldehyde metabolism and biochemical parameters, which define the oxidant-antioxidant system condition and NO metabolism. It has been found that under semicarbazide impact the generation of free radicals, ROS, nitrite and nitrate were enhanced while aldehydes level was reduced that resulted from not only semicarbazide effect like the aldehydes acceptor, but the formaldehyde synthesis slowdown and acceleration of its transformation into format as well. We suppose that formaldehyde plays certain role in the development of connective tissue pathology. 相似文献
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Shapoval L. M. Dmytrenko O. V. Sagach V. F. Prylutska S. V. Khrapatiy S. V. Zavodovskyi D. O. Prylutskyy Yu. I. Tsierkezos N. Ritter U. 《Neurophysiology》2020,52(2):101-109
Neurophysiology - Priority data have been obtained on the effects of repeated systemic administrations of water-dispersible single-walled carbon nanotubes (SWCNTs) to spontaneously hypertensive... 相似文献