排序方式: 共有3条查询结果,搜索用时 15 毫秒
1
1.
Koen Vandelannoote Kurt Jordaens Pieter Bomans Herwig Leirs Lies Durnez Dissou Affolabi Ghislain Sopoh Julia Aguiar Delphin Mavinga Phanzu Kapay Kibadi Sara Eyangoh Louis Bayonne Manou Richard Odame Phillips Ohene Adjei Anthony Ablordey Leen Rigouts Fran?oise Portaels Miriam Eddyani Bouke C. de Jong 《Applied and environmental microbiology》2014,80(3):1197-1209
Buruli ulcer is an indolent, slowly progressing necrotizing disease of the skin caused by infection with Mycobacterium ulcerans. In the present study, we applied a redesigned technique to a vast panel of M. ulcerans disease isolates and clinical samples originating from multiple African disease foci in order to (i) gain fundamental insights into the population structure and evolutionary history of the pathogen and (ii) disentangle the phylogeographic relationships within the genetically conserved cluster of African M. ulcerans. Our analyses identified 23 different African insertion sequence element single nucleotide polymorphism (ISE-SNP) types that dominate in different areas where Buruli ulcer is endemic. These ISE-SNP types appear to be the initial stages of clonal diversification from a common, possibly ancestral ISE-SNP type. ISE-SNP types were found unevenly distributed over the greater West African hydrological drainage basins. Our findings suggest that geographical barriers bordering the basins to some extent prevented bacterial gene flow between basins and that this resulted in independent focal transmission clusters associated with the hydrological drainage areas. Different phylogenetic methods yielded two well-supported sister clades within the African ISE-SNP types. The ISE-SNP types from the “pan-African clade” were found to be widespread throughout Africa, while the ISE-SNP types of the “Gabonese/Cameroonian clade” were much rarer and found in a more restricted area, which suggested that the latter clade evolved more recently. Additionally, the Gabonese/Cameroonian clade was found to form a strongly supported monophyletic group with Papua New Guinean ISE-SNP type 8, which is unrelated to other Southeast Asian ISE-SNP types. 相似文献
2.
Florian Gehre Mebrat Ejo Kristina Fissette Pim de Rijk Cécile Uwizeye Elie Nduwamahoro Odin Goovaerts Dissou Affolabi Martin Gninafon Fanny M. Lingoupou Mamadou Dian Barry Oumou Sow Corinne Merle Piero Olliaro Fatoumata Ba Marie Sarr Alberto Piubello Juergen Noeske Martin Antonio Leen Rigouts Bouke C de Jong 《PloS one》2014,9(12)
In this study, we retrospectively analysed a total of 605 clinical isolates from six West or Central African countries (Benin, Cameroon, Central African Republic, Guinea-Conakry, Niger and Senegal). Besides spoligotyping to assign isolates to ancient and modern mycobacterial lineages, we conducted phenotypic drug-susceptibility-testing for each isolate for the four first-line drugs. We showed that phylogenetically modern Mycobacterium tuberculosis strains are more likely associated with drug resistance than ancient strains and predict that the currently ongoing replacement of the endemic ancient by a modern mycobacterial population in West/Central Africa might result in increased drug resistance in the sub-region. 相似文献
3.
1