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排序方式: 共有106条查询结果,搜索用时 703 毫秒
1.
Aechmea victoriana var discolor L. B. Foster and Aechmea dactylina Bal. are commercially propagated in vitro through lateral shoot growth. A modified Murashige and Skoog medium is used which contains both BA and IAA. These growth substances were shown in the present study to synergistically stimulate the production of ethylene by the cultured plants. The stimulation of ethylene production is correlated with the outgrowth of the lateral buds. The rise in ethylene production was concluded to induce lateral shoot growth, because: (a) outgrowth of the shoots was blocked by preventing an increase in ethylene production, (b) 1-aminocyclopropane-1-carboxylic acid (ACC), the natural precursor of ethylene biosynthesis, substituted for IAA in the promotion of ethylene production and lateral bud outgrowth. Although ACC could substitute for IAA, it could not substitute for BA; therefore, cytokinins are concluded to be essential for lateral bud outgrowth in vitro in Aechmea. These results suggest that cytokinins and ethylene both play roles in natural lateral bud initiation and that the cytokinin function involves two stages of the process. 相似文献
2.
Patrick van Dijck Kristina Schoonjans Paolo Sassone-Corsi Johan Auwerx Guido Verhoeven 《Molecular and cellular biochemistry》1993,125(2):127-136
The hepatic expression of the 2u gene family is controlled by a variety of hormones including steroids, growth hormone and insulin. The mechanisms by which these hormones affect -globulin expression are only partially understood. Recently we isolated and characterized clone RAP 01, an 2u-globulin gene expressed in the liver. In preliminary experiments we noted that partial hepatectomy, a procedure which results in a sharp rise in the level of the oncoproteins c-Fos and c-Jun, also causes a transient induction of the messenger RNA corresponding to clone RAP 01. Using the DNAseI footprinting technique we were able to show that this clone contains a TPA (phorbol 12-myristate 13-acetate)-responsive element (TRE) in its first intron. This element (denoted as element X) is identical to the consensus AP-1 binding site (TGACTCAG) and is protected by rat liver nuclear extracts as well as by purified c-Jun. Gel retardation experiments show that an oligonucleotide containing the TRE consensus sequence competes for binding of liver nuclear proteins to element X and that antibodies directed against the M2 peptide of the mouse Fos protein or the PEP-2 peptide of Jun prevent the formation of specific complexes with the same element. Moreover, element X functions as a TRE in transfected BWTG3 hepatoma cells treated with TPA. Co-transfection withfos andjun expression vectors mimics the effects of TPA suggesting that AP-1 is in fact the mediator of the observed response. It is concluded that the first intron of RAP 01 contains a functional Fos-Jun element. 相似文献
3.
Molecular cloning of a gene involved in glucose sensing in the yeast Saccharomyces cerevisiae 总被引:6,自引:1,他引:5
Linda Van Aelst Stefan Hohmann Botchaka Bulaya Wim de Koning Laurens Sierkstra Maria José Neves Kattie Luyten Rafael Alijo José Ramos Paola Coccetti Enzo Martegani Neuza Maria de Magalhães-Rocha Rogelio Lopes Brandão Patrick Van Dijck Mieke Vanhalewyn Peter Durnez Arnold W. H. Jans Johan M. Thevelein 《Molecular microbiology》1993,8(5):927-943
4.
From data obtained by differential scanning calorimetry phase diagrams were constructed, using a thermodynamically based fitting method. The following binary mixtures of phosphatidylcholines in water were studied: 14:0/14:0-glycerophosphocholine/16:0/16:0-glucerophosphocholine, 14:0/14:0-glycerophosphocholine/18:0/18:0-glycerophosphocholine, 12:0/12:0-glycerophosphocholine/16:0/16:0-glycerophosphocholine, 18:1t/18:1t-glycerophosphocholine/14:0/14:0-glycerophosphocholine and 18:1t/18:1t-glycerophosphocholine/16:0/16:0-glycerophosphocholine. A comparison is made of the present results with those obtained using probe techniques and the differences are discussed. 相似文献
5.
From data obtained by differential scanning calorimetry phase diagrams were constructed, using a thermodynamically based fitting method. The following binary mixtures of phosphatidylcholines in water were studied: 14:0/ 14:0-glycerophosphocholine/16:0/16:0-glycerophosphocholine, 14:0/14:0-glycerophosphocholine /18:0/18:0-glycerophosphocholine, 12:0/12:0-glycerophosphocholine /16:0/16:0-glycerophosphocholine, 18:1t/18:1t-glycerophosphocholine /14:0/14:0-glycerophosphocholine and 18:1t/18:1t-glycerophosphocholine /16:0/16:0-glycerophosphocholine.A comparison is made of the present results with those obtained using probe techniques and the differences are discussed. 相似文献
6.
The sensitivity of different microorganisms, considered as typical representatives of the microflora of soil and water, was established to evaluate the influence of the nonmedical use of antimicrobial agents on bacterial ecology. Only seven strains, six chemoorganotrophs and one chemolithotroph, could be considered as relatively sensitive to the 21 antimicrobial compounds tested. The other 29 microorganisms may be regarded as resistant to most antimicrobial agents. Streptomyces were sensitive to high concentrations of active substances. Broad-spectrum antibiotics showed an effect on environmental bacteria similar to that on human pathogens. Cephalothin stimulated the growth of a Chlorella sp. From these experiments, it appears that spilled antimicrobial agents have little chance of causing an alteration in the microbial ecology. 相似文献
7.
P.W.M. Van Dijck P.H.J.Th. Ververgaert A.J. Verkleij L.L.M. Van Deenen J. De Gier 《生物化学与生物物理学报:生物膜》1975,406(4):465-478
8.
9.
José van Dijck 《New genetics and society》2013,32(1):9-22
Abstract After the birth of Dolly, media stories on cloning were replete with references to well-known science fiction plots. This essay criticizes the ‘imagination deficit’ of scientists and journalists, first by problematizing the uncritical adoption of attenuated science fiction plots in the media coverage of Dolly, and second, by proposing to look at more expansive science fiction novels that carefully examine issues such as uniqueness and identity in relation to the new genetics. 相似文献