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On the Relationship between Ribulose Diphosphate Carboxylase and Protochlorophyllide Holochrome of Phaseolus vulgaris Leaves 总被引:1,自引:1,他引:0 下载免费PDF全文
G. Akoyunoglou J. H. Argyroudi-Akoyunoglou A. Guiali C. Dassiou 《Plant physiology》1970,45(4):443-446
The relationship between ribulose diphosphate carboxylase (3-phospho-d-glycerate carboxy-lyase [dimerizing], EC 4.1.1.39, formerly known as carboxydismutase) and protochlorophyllide holochrome of etiolated Phaseolus vulgaris leaves has been studied.A procedure for partially selective extraction of the two proteins was devised using tris-HCl buffer first without and then with Triton X-100. Ribulose diphosphate carboxylase was readily extracted from etiolated bean leaves without Triton X-100, and protochlorophyllide holochrome was extracted on the addition of Triton X-100.Optimal extraction conditions for protochlorophyllide holochrome have been found to be different for tissues of different ages. 相似文献
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Malamitsi J. Zorzos J. Varvarigou A. D. Archimandritis S. Dassiou C. Sivolapenko G. Skarlos D. V. Serefoglou A. Lykourinas M. Proukakis Ch. 《Cell biochemistry and biophysics》1994,24(1-3):75-81
Ten patients with transitional cell carcinoma (TCC) of the bladder received 3–6 mCi of HMFG1 monoclonal antibody (MAb) intravesically.
The antibody was labeled with Tc-99m using the 2-Iminothiolane method. All patients underwent transurethral resection of the
bladder tumor within 12–20 h following intravesical administration of99m-Tc-HMFG1. The presence of the radiolabeled MAb in the circulation was studied by measuring the radioactivity in the serum
for a period up to 20 h. Three of 10 patients underwent immunoscintigraphy (SPECT) 2–3 h postadministration and cancerous
areas could be easily localized. Biopsies were taken from the tumor sites as well as from normal bladder mucosa. Absolute
uptake of the administered MAb expressed as percent administered dose/kg of tissue could be evaluated only in eight patients.
Multiple specimen taken from various tumor sites in every patient gave a wide range of uptake values ranging from 0 to 9.29%
adm. dose/kg, whereas normal tissue uptake values ranged from 0 to 1.63, respectively. 相似文献
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