排序方式: 共有6条查询结果,搜索用时 22 毫秒
1
1.
Corina Văcăroiu Mădălin Enache Măriuca Gartner Gabriela Popescu Mihai Anastasescu Aurelia Brezeanu N. Todorova Tatiana Giannakopoulou Christos Trapalis Lucia Dumitru 《World journal of microbiology & biotechnology》2009,25(1):27-31
This work focuses on the photocatalytic performances and antibacterial activity of nitrogen doped TiO2 nanosystems with three and five layers obtained by a sol-gel route, followed by thermal treatment in oxygen or ammonia atmosphere
at temperatures between 400 and 1000°C. Subsequently, the antibacterial activity of the obtained nanosystems on the Escherichia coli cells are determined and discussed. The obtained results show a significant dependence of the functional performances on
the system’s composition. In particular, the antimicrobial activity of nitrogen-doped TiO2 films is correlated with the temperature of thermal treatment and illumination time with visible artificial light. 相似文献
2.
We present an analysis of X-ray-induced damage in ΦX174 plasmid DNA, applying doses between D = 250 and 1,500 Gy. To analyse this damage in detail, the distribution of plasmid fragments after irradiation have been determined
by scanning force microscopy. The results show that even for the lowest dose of D = 250 Gy, a significant amount of double-strand breaks are observed. For increasing dose, the percentage of small fragments
increases and is accompanied by a shortening of the average fragment length from < L> = 1,400 nm for a dose of D = 250 Gy to < L> = 1,080 nm after irradiation with D = 1,500 Gy. The most crucial parameter, the average number of double-strand breaks per broken plasmid (<DSBb> ) has been determined for the first time for the applied doses. The results show that the average number of DSBs per broken
plasmid <DSBb> increases almost linearly from a value of <DSBb> = 1.3 after irradiation with D = 250 Gy to <DSBb> = 1.7 after exposure to D = 1,500 Gy. The presented results show that the amount of DSBs induced by X-ray radiation in plasmid DNA can be calculated
with high accuracy by means of scanning force microscopy, providing relevant information regarding the interaction of X-rays
with DNA molecules.
相似文献
M. BrezeanuEmail: |
3.
SFM studies of carbon ion induced damages in plasmid DNA 总被引:1,自引:0,他引:1
Brezeanu M Taucher-Scholz G Psonka K Träger F Hubenthal F 《Journal of molecular recognition : JMR》2007,20(6):502-507
In this study we present for the first time detailed scanning force microscopy (SFM) investigations of carbon ion induced damages in plasmid DNA in order to obtain information about the biological effectiveness of particle radiation. For this purpose, we have combined SFM and gel electrophoresis measurements in a dose range between D = 0 Gy and 5000 Gy. After irradiation with C ions, the percentage of double-strand breaks (DSBs) increases drastically, i.e. from initially 0% for D = 0 Gy to 38% for D = 5000 Gy. Increasing the dose over the total range is accompanied by a shortening of the average fragment length from L = 1100 nm to L = 575 nm. In addition to our experiments, the average numbers of induced DSBs per irradiated plasmid and per broken plasmid have been calculated from the SFM measurements. The most important among the numerous results is that a significant amount of plasmids has suffered more than two DSBs for all applied doses, indicating multiple DSBs. The number of DSBs per broken plasmid increases from approximately 1.7 after irradiation with a dose of D = 250 Gy to 3.2 after exposure to the highest dose of D = 5000 Gy. The results provide experimental data for the spatially correlated production of DSBs after carbon irradiation, that are relevant to the understanding of its biological effectiveness. 相似文献
4.
Elisa Bellucci Orlando Mario Aguilar Saleh Alseekh Kirstin Bett Creola Brezeanu Douglas Cook Lucía De la Rosa Massimo Delledonne Denise F. Dostatny Juan J. Ferreira Valérie Geffroy Sofia Ghitarrini Magdalena Kroc Shiv Kumar Agrawal Giuseppina Logozzo Mario Marino Tristan Mary-Huard Phil McClean Vladimir Meglič Tamara Messer Frédéric Muel Laura Nanni Kerstin Neumann Filippo Servalli Silvia Străjeru Rajeev K. Varshney Marta W. Vasconcelos Massimo Zaccardelli Aleksei Zavarzin Elena Bitocchi Emanuele Frontoni Alisdair R. Fernie Tania Gioia Andreas Graner Luis Guasch Lena Prochnow Markus Oppermann Karolina Susek Maud Tenaillon Roberto Papa 《The Plant journal : for cell and molecular biology》2021,108(3):646-660
5.
6.
Summary The ultrastructure ofOryza sativa L. cells in suspension was determined as cells developed, matured and senesced at 3, 10, and 17 days, respectively, after transfer to fresh medium. Although cultures of 3-day-old cells contained some senescent cells, the symptoms of cell aging were very conspicuous at 10 days and were most pronounced at 17 days. The amount of cytoplasm decreased as the number of lytic areas, myelin figures and vesicle bodies increased. Other noticeable subcellular changes observed were ultrastructural modifications of mitochondria, proplastids, amyloplasts, and nuclei. Such changes were associated with a general deterioration of the lipoprotein complex of the cell during its growth. A fibrous structure without an external membrane was observed and its reported for the first time for cells grown in suspension culture.Mention of a trademark or proprietary product does not constitute a guarantee or warranty of the product by the U.S. Department of Agriculture and does not imply its approval to the exclusion of other products that may also be suitable. 相似文献
1