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排序方式: 共有106条查询结果,搜索用时 15 毫秒
1.
A G Tkachenko A A Chudinov N S Churilova 《Izvestiia Akademii nauk SSSR. Seriia biologicheskaia》1992,(1):42-51
The energetic state and activity of polyamine-synthesizing system of E. coli were studied under conditions of aerobic-anaerobic and pH transitions, nutrition shifts, osmotic and heat shocks. The electron microscopy of cells showed a correlation between cell physiological state and nucleoid ultrastructure. The role of energetic status and polyamines in the regulation of DNA supercoiling is discussed which is a putative cause for the changes observed in the nucleoid ultrastructure. 相似文献
2.
mtDNA diversity in rhesus monkeys reveals overestimates of divergence time and paraphyly with neighboring species 总被引:4,自引:0,他引:4
Reconstructions of the human-African great ape phylogeny by using
mitochondrial DNA (mtDNA) have been subject to considerable debate. One
confounding factor may be the lack of data on intraspecific variation. To
test this hypothesis, we examined the effect of intraspecific mtDNA
diversity on the phylogenetic reconstruction of another Plio- Pleistocene
radiation of higher primates, the fascicularis group of macaque (Macaca)
monkey species. Fifteen endonucleases were used to identify 10 haplotypes
of 40-47 restriction sites in M. mulatta, which were compared with similar
data for the other members of this species group. Interpopulational,
intraspecific mtDNA diversity was large (0.5%- 4.5%), and estimates of
divergence time and branching order incorporating this variation were
substantially different from those based on single representatives of each
species. We conclude that intraspecific mtDNA diversity is substantial in
at least some primate species. Consequently, without prior information on
the extent of genetic diversity within a particular species, intraspecific
variation must be assessed and accounted for when reconstructing primate
phylogenies. Further, we question the reliability of hominoid mtDNA
phylogenies, based as they are on one or a few representatives of each
species, in an already depauperate superfamily of primates.
相似文献
3.
Lapa S. A. Klochikhina E. S. Miftakhov R. A. Zolotov A. M. Zasedatelev A. S. Chudinov A. V. 《Russian Journal of Bioorganic Chemistry》2020,46(5):859-861
Russian Journal of Bioorganic Chemistry - A multiplex PCR system has been developed and optimized for rapid detection of the five main pathogens of bacterial pneumonia. The system can be expanded... 相似文献
4.
We analyzed the possibility of triplex formation in the human alpha 1-antitrypsin gene. Conditions and nucleotide sequence dependence of triplex formation and thermostability of the product were determined. 相似文献
5.
Barsky V Perov A Tokalov S Chudinov A Kreindlin E Sharonov A Kotova E Mirzabekov A 《Journal of biomolecular screening》2002,7(3):247-257
A series of biochip readers developed for gel-based biochips includes three imaging models and a novel nonimaging biochip scanner. The imaging readers, ranging from a research-grade versatile reader to a simple portable one, use wide-field objectives and 12-bit digital large-coupled device cameras for parallel addressing of multiple array elements. This feature is valuable for monitoring the kinetics of sample interaction with immobilized probes. Depending on the model and the label used, the sensitivity of these readers approaches 0.3 amol of a labeled sample per gel element. In the selective scanner, both the spot size of the excitation laser beam and the detector field of view match the size of the biochip array elements so that the whole row of the array can be read in a single scan. The portable version reads 50-mm long, 150-element, one-dimensional arrays in 5 s. With a dynamic range of 4000:1, a sensitivity of 1-5 amol of a labeled sample per gel element, and a data format facilitating online processing, the scanner is an attractive, inexpensive solution for biomedical diagnostics. Fluorophores for sample labeling were compared experimentally in terms of detection sensitivity, influence on duplex stability, and suitability for multilabel analysis and thermodynamic studies. Texas Red and tetracarboxyphenylporphyn proved to be the best choice for two-wavelength analysis using the imaging readers. 相似文献
6.
Griadunov DA Mikhaĭlovich VM Lapa SA Rudinskiĭ NI Barskiĭ VE Chudinov AV Zasedatelev AS Mirzabekov AD 《Molekuliarnaia genetika, mikrobiologiia i virusologiia》2003,(4):24-27
A method of multiplex polymerase chain reaction (PCR) with subsequent hyoridization on oligonucleotide microchips was worked out to identify the Mycobacterium tuberculosis complex and to determine simultaneously the bacterial sensitivity to 2 first-line drugs, i.e. rifampin and isoniazid. The method provides for detecting above 95% of rifampin-resistant and around 80% of isoniazid-resistant strains within 1 day. 相似文献
7.
Alexandrova LA Jasko MV Belobritskaya EE Chudinov AV Mityaeva ON Nasedkina TV Zasedatelev AS Kukhanova MK 《Bioconjugate chemistry》2007,18(3):886-893
A simple and convenient method for incorporation of fluorescent or ligand groups into 3'-termini of DNA fragments is proposed. A set of triphosphoric acid monoesters bearing fluorescent groups or biotin attached to the triphosphate fragment through linkers of different lengths and structures was synthesized. All the compounds were substrates for calf thymus terminal deoxynucleotidyltransferase and were used for incorporation of marker groups into 3'-termini of DNA fragments. The compounds were successfully applied for DNA labeling during post-PCR target preparation for microarray analysis. 相似文献
8.
Lapa S. A. Volkova O. S. Kuznetsova V. E. Zasedatelev A. S. Chudinov A. V. 《Molecular Biology》2022,56(1):115-123
Molecular Biology - The substrate properties of nitrogen-base modified derivatives of purine and pyrimidine deoxynucleoside triphosphates during their simultaneous pairwise insertion into the... 相似文献
9.
Shershov V. E. Kuznetsova V. E. Lysov Yu. P. Guseinov T. O. Barsky V. E. Spitsyn M. A. Zasedateleva O. A. Vasiliskov V. A. Surzhikov S. A. Zasedatelev A. S. Chudinov A. V. 《Biophysics》2015,60(6):1013-1015
Biophysics - The effect of the chromophore charge on the efficiency of incorporation of fluorescent-labeled nucleotides into DNA during PCR was studied using three dUTP derivatives that contain... 相似文献
10.
The methods of synthesis of the derivatives of nucleoside analogues esterified with various aliphatic, aromatic, and heteroaromatic acids and the construction from them of molecular transport systems that involve lipids, carbohydrates, peptides, and amino acids are discussed. The characteristics of the biological activity of a number of such systems are described. 相似文献