Gli1 interacts with YAP1 to promote tumorigenesis in esophageal squamous cell carcinoma

Esophageal squamous cell carcinoma (ESCC) is the predominant esophageal cancer type in China. The aberrant activation of glioma-associated oncogene homolog1 (Gli1), a key factor in Hedgehog (Hh) signaling pathway, has been found in esophageal carcinoma. Moreover, Yes-associated protein 1 (YAP1), the major mediator of Hippo signaling pathway, has been linked to esophageal carcinoma progression. However, the precise roles and the underlying mechanism of both Gli1 and YAP1 in ESCC are unclear. Here, we found that Gli1 and YAP1 are overexpressed in ESCC and are associated with poor prognosis. In addition, we confirmed that knockdown of Gli1 or YAP1 suppresses ESCC cell growth, migration, and invasion in ESCC TE1 and EC109 cells. Significantly, Gli1 interacts with YAP1 in ESCC cells. Both Gli1 and YAP1 proteins are closely correlated with each other in human ESCC samples. Mechanistically, Gli1 upregulates YAP1 in a LATS1-independent manner. Conversely, YAP1 induces Gli1 by regulating phosphoinositide 3-kinase (PI3K)/AKT signaling pathway. Most importantly, we demonstrated that the interaction between Gli1 and YAP1 promotes ESCC tumor growth in vitro and in vivo. Our findings established a novel signaling mechanism by which the interaction between Gli1 and YAP1 promotes ESCC cell growth. This signaling regulation of the tumorigenesis provides a new therapeutic strategy for highly lethal ESCC.     

Paecilomyces variotii extracts (ZNC) enhance plant immunity and promote plant growth

Plant and Soil - There are growing concerns regarding the restoration of karst rocky desertification (KRD) areas. However, the soil conditions and its residing microorganisms, which are essential...     

Gankyrin activates the hedgehog signalling to drive metastasis in osteosarcoma

Gankyrin is a regulatory subunit of the 26-kD proteasome complex and promotes the occurrence and progression of many malignancies. However, the role of gankyrin in osteosarcoma (OS) metastasis remains unclear. Hedgehog signalling has been shown to regulate stem cell homeostasis and cancer metastasis, but the mechanisms that activate this pathway in OS are still poorly understood. Here, a series of in vitro and in vivo assays were carried out to explore the function and mechanism of gankyrin regulating Hedgehog signalling in OS. We demonstrated that gankyrin promotes migration, invasion and regulates the expression of some stemness factors by up-regulating Gli1 in OS. Importantly, our data showed an interaction between gankyrin and Gli1. Moreover, gankyrin suppresses the ubiquitin-mediated degradation of Gli1 protein in OS. Gankyrin also significantly promotes the lung metastasis of OS in vivo. Our findings suggest that gankyrin drives metastasis and regulates the expression of some stemness factors in osteosarcoma by activating Hedgehog signalling, indicating that drug screening for compounds targeting gankyrin may contribute to the development of novel and effective therapies for OS.     

Blocking the flow of propionate into TCA cycle through a mutB knockout leads to a significant increase of erythromycin production by an industrial strain of Saccharopolyspora erythraea

Bioprocess and Biosystems Engineering - A high erythromycin producing mutant strain Saccharopolyspora erythraea HL3168 E3-ΔmutB was constructed by deleting mutB (SACE_5639) gene encoding the...     

Loss of Spike N370 glycosylation as an important evolutionary event for the enhanced infectivity of SARS-CoV-2

Dear Editor, SARS-CoV-2 belongs to the Sarbecovirus subgenus of betacor-onaviruses and other members in this subgenus include SARS-CoV and coronaviruses mainly ...     

Correction to: High‑Throughput MicroRNA and mRNA Sequencing Reveals that MicroRNAs may be Involved in Peroxidase‑Mediated Cold Tolerance in Potato

Plant Molecular Biology Reporter -     

High-Throughput MicroRNA and mRNA Sequencing Reveals that MicroRNAs may be Involved in Peroxidase-Mediated Cold Tolerance in Potato

Plant Molecular Biology Reporter - Low-temperature is one of the most severe abiotic stresses affecting the potato production as the cultivated potato (Solanum tuberosum) is frost sensitive....     

Citrullyl-Hydroxyprolyl-Proline (ChPP): An Artificially Synthesized Tripeptide as Potent ACE Inhibitor

International Journal of Peptide Research and Therapeutics - Currently bioactive peptides are the main focus in attempts to identify novel angiotensin-converting enzyme inhibitors (ACEIs) for the...     

羊流产嗜衣原体ompA基因克隆及生物信息学分析

分析羊流产嗜衣原体ompA基因结构并预测其编码蛋白的结构和功能。采用DNA Star、DNA MAN、vector NTI suite11.5序列分析软件和在线网站ExPASy分析该基因的结构和预测其编码蛋白的理化性质、亚细胞定位、一级结构修饰位点、二级结构特征及三维空间构象、潜在抗原表位等。结果显示,该基因全长1 170 bp,可编码389个氨基酸,编码蛋白理化性质较稳定,无各种亚细胞定位序列,含有多个能被其他酶修饰的位点,该蛋白以无规则卷曲为主,大部分氨基酸残基包埋在分子内部,含5个跨膜区,3个亲水性较强的抗原表位。ompA基因生物信息学分析结果为ompA蛋白功能的深入研究和新型多价疫苗的开发提供了基础数据。