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Murine monoclonal antibodies specific for conserved and non-conserved antigenic determinants of the human and murine Ku autoantigens 总被引:5,自引:0,他引:5
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Wu Po-Ming Lin Chih-Hao Lee Hsueh-Te Shih Hsin-I Huang Chao-Ching Tu Yi-Fang 《Neurochemical research》2020,45(11):2712-2722
Neurochemical Research - Neonatal hypoxic–ischemic encephalopathy is the most common cause of neurological disability in infancy. Superimposed inflammation may further worsen neurological... 相似文献
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Chun-Li Lin Jian-Hong Yu Heng-Liang Liu Chih-Hao Lin Yang-Sung Lin 《Journal of biomechanics》2010,43(11):2174-2181
This study determines the relative effects of changes in bone/mini-screw osseointegration and mini-screw design factors (length, diameter, thread shape, thread depth, material, head diameter and head exposure length) on the biomechanical response of a single mini-screw insertion. Eighteen CAD and finite element (FE) models corresponding to a Taguchi L18 array were constructed to perform numerical simulations to simulate mechanical responses of a mini-screw placed in a cylindrical bone. The Taguchi method was employed to determine the significance of each design factor in controlling strain. Simulation results indicated that mini-screw material, screw exposure length and screw diameter were the major factors affecting bone strain, with percentage contributions of 63%, 24% and 7%, respectively. Bone strain decreased obviously when screw material had the high elastic modulus of stainless/titanium alloys, a small exposure length and a large diameter. Other factors had no significanton bone strain. The FE analysis combined with the Taguchi method efficiently identified the relative contributions of several mini-screw design factors, indicating that using a strong stainless/titanium alloys as screw material is advantageous, and increase in mechanical stability can be achieved by reducing the screw exposure length. Simulation results also revealed that mini-screw and bone surface contact can provide sufficient mechanical retention to perform immediately load in clinical treatment. 相似文献
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Disrupted-In-Schizophrenia 1 regulates integration of newly generated neurons in the adult brain 总被引:10,自引:0,他引:10
Duan X Chang JH Ge S Faulkner RL Kim JY Kitabatake Y Liu XB Yang CH Jordan JD Ma DK Liu CY Ganesan S Cheng HJ Ming GL Lu B Song H 《Cell》2007,130(6):1146-1158
Adult neurogenesis occurs throughout life in discrete regions of the adult mammalian brain. Little is known about the mechanism governing the sequential developmental process that leads to integration of new neurons from adult neural stem cells into the existing circuitry. Here, we investigated roles of Disrupted-In-Schizophrenia 1 (DISC1), a schizophrenia susceptibility gene, in adult hippocampal neurogenesis. Unexpectedly, downregulation of DISC1 leads to accelerated neuronal integration, resulting in aberrant morphological development and mispositioning of new dentate granule cells in a cell-autonomous fashion. Functionally, newborn neurons with DISC1 knockdown exhibit enhanced excitability and accelerated dendritic development and synapse formation. Furthermore, DISC1 cooperates with its binding partner NDEL1 in regulating adult neurogenesis. Taken together, our study identifies DISC1 as a key regulator that orchestrates the tempo of functional neuronal integration in the adult brain and demonstrates essential roles of a susceptibility gene for major mental illness in neuronal development, including adult neurogenesis. 相似文献
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Disulfide bonds play an important role in stabilizing protein structure and regulating protein function. Therefore, the ability to infer disulfide connectivity from protein sequences will be valuable in structural modeling and functional analysis. However, to predict disulfide connectivity directly from sequences presents a challenge to computational biologists due to the nonlocal nature of disulfide bonds, i.e., the close spatial proximity of the cysteine pair that forms the disulfide bond does not necessarily imply the short sequence separation of the cysteine residues. Recently, Chen and Hwang (Proteins 2005;61:507-512) treated this problem as a multiple class classification by defining each distinct disulfide pattern as a class. They used multiple support vector machines based on a variety of sequence features to predict the disulfide patterns. Their results compare favorably with those in the literature for a benchmark dataset sharing less than 30% sequence identity. However, since the number of disulfide patterns grows rapidly when the number of disulfide bonds increases, their method performs unsatisfactorily for the cases of large number of disulfide bonds. In this work, we propose a novel method to represent disulfide connectivity in terms of cysteine pairs, instead of disulfide patterns. Since the number of bonding states of the cysteine pairs is independent of that of disulfide bonds, the problem of class explosion is avoided. The bonding states of the cysteine pairs are predicted using the support vector machines together with the genetic algorithm optimization for feature selection. The complete disulfide patterns are then determined from the connectivity matrices that are constructed from the predicted bonding states of the cysteine pairs. Our approach outperforms the current approaches in the literature. 相似文献
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Novel method of forming human embryoid bodies in a polystyrene dish surface-coated with a temperature-responsive methylcellulose hydrogel 总被引:1,自引:0,他引:1
A temperature-responsive hydrogel composed of aqueous methylcellulose (MC) blended with distinct concentrations of PBS was prepared and characterized. The developed MC hydrogel underwent a sol-gel reversible transition upon heating or cooling at approximately 32 degrees C. This temperature-responsive hydrogel was employed to coat the surface of a polystyrene dish and used to cultivate human embryonic stem (hES) cell clumps for the formation of embryoid bodies (EBs) in liquid suspension culture (LSC-MC/PS). The conventional hanging drop culture (HDC) and LSC in the uncoated polystyrene dish (LSC-PS) or in the Corning Ultralow-Attachment plate (LSC-ULAP) were used as controls. The results indicated that LSC-PS failed to generate EBs in an efficient manner, whereas the efficiencies of EB formation observed in LSC-ULAP and LSC-MC/PS were significantly greater than in HDC. The hES cells within the EBs were shown to express molecular markers specific for representative cells from the three embryonic germ layers. These results indicated that the MC-coated dish can be used to produce a large scale of hES cell derivatives through the formation of EBs. 相似文献
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CY Huang CM Shih NW Tsao YH Chen CY Li YJ Chang NC Chang KL Ou CY Lin YW Lin CH Nien FY Lin 《PloS one》2012,7(8):e42808
The expression of vascular adhesion molecule-1 (VCAM-1) by endothelial cells may play a major role in atherogenesis. The actual mechanisms of chlamydia pneumoniae (C. pneumoniae) relate to atherogenesis are unclear. We investigate the influence of VCAM-1 expression in the GroEL1 from C. pneumoniae-administered human coronary artery endothelial cells (HCAECs) and hypercholesterolemic rabbits. In this study, we constructed the recombinant GroEL1 from C. pneumoniae. The HCAECs/THP-1 adhesion assay, tube formation assay, western blotting, enzyme-linked immunosorbent assay, actinomycin D chase experiment, luciferase reporter assay, and immunohistochemical stainings were performed. The results show that GroEL1 increased both VCAM-1expression and THP-1 cell adhesives, and impaired tube-formation capacity in the HCAECs. GroEL1 significantly increased the VCAM-1 mRNA stability and cytosolic AU-binding factor 1 (AUF1) level. Overexpression of the p37(AUF1) significantly increased VCAM-1 gene expression in GroEL1-induced bovine aortic endothelial cells (BAECs). GroEL1 prolonged the stability of VCAM-1 mRNA by increasing both p37(AUF1) and the regulation of the 5' untranslated region (UTR) of the VCAM-1 mRNA in BAECs. In hypercholesterolemic rabbits, GroEL1 administration enhanced fatty-streak and macrophage infiltration in atherosclerotic lesions, which may be mediated by elevated VCAM-1 expression. In conclusion, GroEL1 induces VCAM-1 expression by p37(AUF1) in endothelial cells and enhances atherogenesis in hypercholesterolemic rabbits. 相似文献
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