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1.
N-Methyl-D-Aspartate receptors in acquisition of the short-term memory in the honeybeeApis mellifera
N. G. Lopatina I. V. Ryzhova E. G. Chesnokova L. A. Dmitrieva 《Journal of Evolutionary Biochemistry and Physiology》2000,36(3):294-300
Role of NMDA receptors in the process of associative learning has been studied in the honeybeeApis mellifera L. in behavior experiments, using method of conditional reflexes. To determine pharmacological profile of NMDA receptors,
effects of Mg2+ ions, NMDA, glycine (Gly), antagonist of the glycine site 5,7-dichlorokynurenic acid (DCK), competitive antagonists of NMDA
receptors: D,L-2-aminophosphovalerate (APV), L-2-aminophosphobutyrate (APB), and D-glutamyl-aminomethylphosphonic acid (GAMP)
as well of antagonists of NMDA receptor ion channels MK-801 and ketamine (Ket) were studied on acquisition of alimentary conditional
reflex and its retention in memory. NMDA increased capacity for learning by stimulating shortterm memory. The NMDA receptor
co-agonist Gly activated this NMDA effect. DCK eliminated the NMDA and Gly stimulatoty effects. All tested antagonists at
millimolar concentrations inhibited associative function. The data obtained confirm our hypothesis about participation of
NMDA receptors in processes of formation of short-term memory in the honeybeeApis mellifera and suggest that functional characteristics of the NMDA receptors involved in the process of associative learning in the
honeybee resemble those in mammals. 相似文献
2.
Yu. S. Ravkin S. M. Tsybulin S. G. Livanov K. V. Toropov V. N. Kuranova V. P. Starikov S. V. Chesnokova 《Contemporary Problems of Ecology》2008,1(5):568-573
For the entire territory of West Siberia, including its plain and mountainous parts, two maps were constructed for populations of amphibians together with reptiles, and of birds. Communities of these classes of vertebrates differ from one another in their spatial differentiation. The differences are demonstrated for the plain and mountainous territories considered jointly and separately. 相似文献
3.
immunocytochemical localization of urokinase-type plasminogen activator in lewis lung carcinoma 总被引:1,自引:0,他引:1 下载免费PDF全文
L Skriver LI Larsson V Kielberg LS Nielsen PB Andresen P Kristensen K Dano 《The Journal of cell biology》1984,99(2):753-758
The invasively growing and metasizing Lewis lung carcinoma consistently contained urokinase-type plasminogen activator (u-PA) enzyme activity. When investigated immunocytochemically with antibodies against u-PA, different parts of individual tumors showed a pronounced heterogeneity in staining intensity. Strong staining was found in areas with invasive growth and degradation of surrounding normal tissue, while other areas were completely devoid of staining. Immunoreactivity occurred both with a perinuclear cytoplasmic localization in tumor cells and associated with apparently extracellular material. SDS PAGE of tumor extracts, under both reducing and nonreducing conditions, followed by immunoblotting, showed only one immunocytochemically stainable band with an electrophoretic mobility corresponding to that of purified proenzyme to u-PA, while no two-chain u-PA was detected. This indicates that the major part of the activator in Lewis lung carcinoma is present as one-chain pro-u-PA. 相似文献
4.
5.
A. P. Bonartsev V. V. Voinova E. S. Kuznetsova I. I. Zharkova T. K. Makhina V. L. Myshkina D. V. Chesnokova K. S. Kudryashova A. V. Feofanov K. V. Shaitan G. A. Bonartseva 《Applied Biochemistry and Microbiology》2018,54(4):379-386
Porous scaffolds for tissue engineering have been prepared from poly(3-hydroxybutyrate) (PHB) and a copolymer of poly(3-hydroxybutyrate) and polyethylene glycol (PHB-PEG) produced by bioPEGylation. The morphology of the scaffolds and their capacity for adsorption of the model protein bovine serum albumin (BSA) have been studied. Scaffolds produced from bioPEGylated PHB adsorbed more BSA, whereas the share of protein irreversibly adsorbed on these scaffolds was significantly lower (33%) than in the case of PHB homopolymer-based scaffolds (47%). The effect of protein adsorption on scaffold biocompatibility in vitro was tested in an experiment that involved the cultivation of fibroblasts (line COS-1) on the scaffolds. PHB-PEG scaffolds had a higher capacity for supporting cell growth than PHB-based scaffolds. Thus, the bioPEGylated PHB-based polymer scaffolds developed in the present study have considerable potential for use in soft tissue engineering. 相似文献
6.
7.
Abstract Post-construction avifauna investigations were undertaken at Project West Wind, Meridian Energy Limited's 62-turbine wind farm on the Wellington south coast. These investigations were required in accordance with the resource consent conditions to quantify the level of avian mortalities occurring at the wind farm, particularly in regard to New Zealand falcon (Falco novaeseelandiae), kākā (Nestor meridionalis) and kererū (Hemiphaga novaeseelandiae). This is the first comprehensive study at a New Zealand operating wind farm. The methods included three field components necessary to calculate annual estimates of mortalities across the wind farm site: routine turbine searches; carcass detection trials; and carcass removal trials. Results from years 1 and 2 of a three-year programme are presented. To date, mortalities have been recorded for 17 taxa at 18 of the 24 study turbines. There have been no recorded mortalities of falcon, kākā or kererū. Australasian harrier (Circus approximans) has been the species for which the most mortalities have been recorded. Overall estimated annual mortality rates for years 1 and 2 were calculated to be approximately six and five birds per turbine respectively. 相似文献
8.
The additive genetic effects of traits can be used to predict evolutionary trajectories,
such as responses to selection. Non-additive genetic and maternal environmental effects
can also change evolutionary trajectories and influence phenotypes, but these effects have
received less attention by researchers. We partitioned the phenotypic variance of survival
and fitness-related traits into additive genetic, non-additive genetic and maternal
environmental effects using a full-factorial breeding design within two allopatric
populations of Atlantic salmon (Salmo salar). Maternal environmental effects were
large at early life stages, but decreased during development, with non-additive genetic
effects being most significant at later juvenile stages (alevin and fry). Non-additive
genetic effects were also, on average, larger than additive genetic effects. The
populations, generally, did not differ in the trait values or inferred genetic
architecture of the traits. Any differences between the populations for trait values could
be explained by maternal environmental effects. We discuss whether the similarities in
architectures of these populations is the result of natural selection across a common
juvenile environment. 相似文献
9.
10.
Klimov VT Chesnokova MV Breneva NV Maramovich AS 《Molekuliarnaia genetika, mikrobiologiia i virusologiia》2002,(4):39-41
A mode of feces sample preparation was developed for polymerase chain reaction (PCR) assay. It was based on alkaline treatment of the material. This treatment killed the most part of indigenous microflora, whereas Yersinia survived, because it was relatively resistant to alkaline. The mode was tested using human feces artificially contaminated with Yersinia pseudotuberculosis. Positive responses in samples containing 10(3)-10(8) microbial cells per ml were obtained by PCR assay with Yersi and Yers2, Invl and Inv2, YP3 and YP4 primers. Diagnostic efficiency of PCR for patients, small mammals, and washings from environmental objects was 4.75, 1.66, and 2.12 times higher than diagnostic efficiency of bacteriological analysis of these samples, respectively. Positive results in PCR were obtained at the day of the material collection and treatment, whereas Y. pseudotuberculosis was isolated only after 8-20 days. Positive samples in PCR and in bacteriological analysis were found to coincide. A brief scheme of the Y. pseudotuberculosis laboratory diagnosis is suggested. According to this scheme, target-oriented bacteriological assay is performed only in those samples, in which preliminary PCR assay after 1-3 days of incubation gave positive results of Y. pseudotuberculosis DNA detection. 相似文献