排序方式: 共有3条查询结果,搜索用时 15 毫秒
1
1.
2.
3.
Chaewon Bae Hiroshi Katoh Aika Wakata Masafumi Sakata Fumihiro Kato Makoto Takeda 《Microbiology and immunology》2023,67(1):44-47
The reverse genetics system is a very powerful tool for analyzing the molecular mechanisms of viral propagation and pathogenesis. However, full-length genome plasmid construction is highly time-consuming and laborious, and undesired mutations may be introduced by Escherichia coli. This study shows a very rapid E. coli-free method of full-genome construction using the mumps virus as an example. This method was able to reduce dramatically the time for full-genome construction, which was used very efficiently for virus rescue, from several days or more to ~2 days, with a similar accuracy and yield to the conventional method using E. coli/plasmid. 相似文献
1