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Inês Carqueijeiro Thomas Dugé de Bernonville Arnaud Lanoue Thu‐Thuy Dang Christiana N Teijaro Christian Paetz Kevin Billet Angela Mosquera Audrey Oudin Sébastien Besseau Nicolas Papon Gaëlle Glévarec Lucía Atehortùa Marc Clastre Nathalie Giglioli‐Guivarc'h Bernd Schneider Benoit St‐Pierre Rodrigo B. Andrade Sarah E. O'Connor Vincent Courdavault 《The Plant journal : for cell and molecular biology》2018,94(3):469-484
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Ferreres F Figueiredo R Bettencourt S Carqueijeiro I Oliveira J Gil-Izquierdo A Pereira DM Valentão P Andrade PB Duarte P Barceló AR Sottomayor M 《Journal of experimental botany》2011,62(8):2841-2854
Class III peroxidases (Prxs) are plant enzymes capable of using H(2)O(2) to oxidize a range of plant secondary metabolites, notably phenolic compounds. These enzymes are localized in the cell wall or in the vacuole, which is a target for secondary metabolite accumulation, but very little is known about the function of vacuolar Prxs. Here, the physiological role of the main leaf vacuolar Prx of the medicinal plant Catharanthus roseus, CrPrx1, was further investigated namely by studying its capacity to oxidize co-localized phenolic substrates at the expense of H(2)O(2). LC-PAD-MS analysis of the phenols from isolated leaf vacuoles detected the presence of three caffeoylquinic acids and four flavonoids in this organelle. These phenols or similar compounds were shown to be good CrPrx1 substrates, and the CrPrx1-mediated oxidation of 5-O-caffeoylquinic acid was shown to form a co-operative regenerating cycle with ascorbic acid. Interestingly, more than 90% of total leaf Prx activity was localized in the vacuoles, associated to discrete spots of the tonoplast. Prx activity inside the vacuoles was estimated to be 1809 nkat ml(-1), which, together with the determined concentrations for the putative vacuolar phenolic substrates, indicate a very high H(2)O(2) scavenging capacity, up to 9 mM s(-1). Accordingly, high light conditions, known to increase H(2)O(2) production, induced both phenols and Prx levels. Therefore, it is proposed that the vacuolar couple Prx/secondary metabolites represent an important sink/buffer of H(2)O(2) in green plant cells. 相似文献
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Lemos Cruz Pamela Carqueijeiro Ines Koudounas Konstantinos Bomzan Dikki Pedenla Stander Emily Amor Abdallah Cécile Kulagina Natalja Oudin Audrey Lanoue Arnaud Giglioli-Guivarc’h Nathalie Nagegowda Dinesh A Papon Nicolas Besseau Sébastien Clastre Marc Courdavault Vincent 《Protoplasma》2023,260(2):607-624
Protoplasma - The medicinal plant Catharanthus roseus biosynthesizes many important drugs for human health, including the anticancer monoterpene indole alkaloids (MIAs) vinblastine and vincristine.... 相似文献
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Natalja Kulagina Grégory Guirimand Céline Melin Pamela Lemos-Cruz Ines Carqueijeiro Johan-Owen De Craene Audrey Oudin Vladimir Heredia Konstantinos Koudounas Marianne Unlubayir Arnaud Lanoue Nadine Imbault Benoit St-Pierre Nicolas Papon Marc Clastre Nathalie Giglioli-Guivarc’h Jillian Marc Sébastien Besseau Vincent Courdavault 《Microbial biotechnology》2021,14(6):2693-2699
The pharmaceutical industry faces a growing demand and recurrent shortages in many anticancer plant drugs given their extensive use in human chemotherapy. Efficient alternative strategies of supply of these natural products such as bioproduction by microorganisms are needed to ensure stable and massive manufacturing. Here, we developed and optimized yeast cell factories efficiently converting tabersonine to vindoline, a precursor of the major anticancer alkaloids vinblastine and vincristine. First, fine-tuning of heterologous gene copies restrained side metabolites synthesis towards vindoline production. Tabersonine to vindoline bioconversion was further enhanced through a rational medium optimization (pH, composition) and a sequential feeding strategy. Finally, a vindoline titre of 266 mg l−1 (88% yield) was reached in an optimized fed-batch bioreactor. This precursor-directed synthesis of vindoline thus paves the way towards future industrial bioproduction through the valorization of abundant tabersonine resources. 相似文献
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Inês Carqueijeiro Konstantinos Koudounas Thomas Dug de Bernonville Liuda Johana Sepúlveda Angela Mosquera Dikki Pedenla Bomzan Audrey Oudin Arnaud Lanoue Sbastien Besseau Pamela Lemos Cruz Natalja Kulagina Emily A Stander Sbastien Eymieux Julien Burlaud-Gaillard Emmanuelle Blanchard Marc Clastre Lucia Atehorta Benoit St-Pierre Nathalie Giglioli-Guivarch Nicolas Papon Dinesh A Nagegowda Sarah E OConnor Vincent Courdavault 《Plant physiology》2021,185(3):836
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