Isolation of polymorphic tetranucleotide microsatellite markers for the ornate nursery frog Cophixalus ornatus

We have isolated 18 polymorphic microsatellite loci for Cophixalus ornatus from genomic libraries enriched for (AAAG)_n, (AACC)_n and (AAGG)_n repetitive elements. The number of alleles ranges from five to 22 per locus with the observed heterozygosity ranging from 0.10 to 0.92. These markers will be useful for the analysis of population structure in C. ornatus and testing alternative models of speciation.     

Isolation of polymorphic tetranucleotide microsatellite markers for the little greenbul (Andropadus virens)

Thirteen polymorphic microsatellite markers have been developed for the little greenbul, Andropadus virens. The number of alleles range from two to 15 per locus with the observed heterozygosity ranging from 0.15 to 0.92. Eight sets of primers also amplify in closely related species, Andropadus laterostris and Andropadus tephrolaemus. These markers will be useful for analysis of questions concerning population genetic structure and models of speciation.     

Effects of Nutrient Supply on Pre-emergence Growth and Nutrient Absorption in Wheat (Triticum aestivumL.) and Sugarbeet (Beta vulgarisL.)

Nutrient absorption in wheat and sugarbeet was studied duringpre-emergence growth by adding 0, 7, 10.5 or 14 mol m^-3nitrogen(N) to the growth medium. Seedling growth and carbon, N and¹⁵Ncontents of the seedling parts were measured. Differences betweenthe natural abundance of¹⁵N in seeds and in nutrient solutionwere used to determine the proportion of N in the organs originatingfrom seed reserves and from absorption. Absorption began laterfor wheat than for sugarbeet and had less effect on seedlinggrowth. The absorbed N was found mainly in roots. Compared towheat, sugarbeet seedling N content was greatly altered andthe hypocotyl showed increased elongation when nutrients wereadded. Most of the absorbed N was found in the radicle and hypocotylwith less in the cotyledons. Sugarbeet seedling emergence andearly growth could be decreased by adverse conditions occurringafter sowing by affecting mineral availability in the soil orthrough altered root absorption.Copyright 1998 Annals of BotanyCompany Triticum aestivumL., wheat,Beta vulgarisL., emergence, natural isotopic composition, seedling, seed reserves     

Isolation of polymorphic tetranucleotide microsatellite markers for the large‐billed scrubwren (Sericornis magnirostris)

We isolated 12 polymorphic microsatellite markers for the large‐billed scrubwren Sericornis magnirostris from genomic libraries enriched for (AAGG)_n and (AACC)_n repetitive elements and characterized them in 11 individuals. The number of alleles ranged from four to 15 per locus with the observed heterozygosity ranging from 0.14 to 0.91. These markers will be useful to address questions concerning population genetic structure and models of speciation.     

Isolation of polymorphic tetranucleotide microsatellite markers for the streak‐necked flycatcher Mionectes striaticollis

We have isolated 11 polymorphic microsatellite markers for the streak‐necked flycatcher Mionectes striaticollis from genomic libraries enriched for either (AACC)_n, (AAGG)_n or (AAAG)_n repetitive elements. The number of alleles ranged from four to 14 per locus with the observed heterozygosity ranging from 0.38 to 1.00. These markers will be useful for analysis of questions concerning population genetic structure and models of speciation.     

Isolation of polymorphic tetranucleotide microsatellite markers in the satin bowerbird,Ptilonorhynchus violaceus

We isolated 13 polymorphic microsatellite markers from the satin bowerbird, Ptilonorhynchus violaceus from a genomic library enriched in (AAGG)_n repetitive elements and characterized them in 20 individuals. The number of alleles ranged from two to 18 per locus with the observed heterozygosity ranging from 0.15 to 1.00. These markers will be useful for analysing questions concerning parentage, population genetic structure and models of speciation.     

Intraspecific variation in Anolis sagrei mirrors the adaptive radiation of Greater Antillean anoles

Anolis lizards of the Greater Antilles represent one of the classic examples of vertebrate adaptive radiation. The same morphological types ('ecomorphs') have evolved repeatedly in response to similar ecological pressures on different islands. We tested whether patterns of within species diversification were congruent with between species patterns and the processes leading to the adaptive radiation of Greater Antillean anoles by measuring variation in performance-related morphological characters in the brown anole, Anolis sagrei . We measured morphological and genetic variation in two different habitat types on each of five islands in the Bahamas. We estimated population structure and rates of gene flow within and among islands using eight microsatellite markers. Intraspecific variation in performance-related morphological characters was similar to the pattern of interspecific variation that characterizes the adaptive radiation of this group in the Greater Antilles. For example, limb length was correlated with perch diameter within A. sagrei as has also been shown among species of anole. Morphological divergence in traits has occurred despite relatively high levels of gene flow both within and among islands. These results are discussed in the context of the divergence-with-gene-flow model of speciation. The results provide important intraspecific evidence that the diversification of anoles has been shaped by natural selection and show how ecologically-based selection pressures explain diversification at both the population and species levels.  © 2007 The Linnean Society of London, Biological Journal of the Linnean Society , 2007, 90 , 189–199.     

Pre-emergence Growth of Genotypes of Sugarbeet (Beta vulgaris L.) Tolerant to Rhizomania

Growth during pre-emergence of three sugarbeet genotypes, alltolerant to the root disease rhizomania but with poor emergencefeatures, was studied. Dry mass, carbon and nitrogen content,and N isotope composition were measured for the seeds and eachseedling part. These results were compared with those obtainedfor Vega, a genotype with no emergence problems. The depletionof seed reserves and carbon use were similar for all genotypes.Tolerant genotypes had altered nitrogen absorption: the netnitrogen balance (total N in the seedling/N initial mass inthe seed) was 20% lower in these genotypes at the end of theexperiments. Differences in the natural abundance of¹⁵N in seedsand nutrient solutions were used to determine the proportionof N originating from seed reserves or from absorption by theorgans. N absorbed from the nutrient solution was found mainlyin the hypocotyl and radicle, with less in the cotyledons ofall genotypes. Tolerant genotypes had less absorbed N in allparts than non-tolerant genotypes. Hypocotyl and radicle elongationwas the same in all genotypes, but the emergence forces in rhizomania-tolerantgenotypes were smaller. These results suggest that breedingto introduce tolerance to rhizomania has also led to a loweredmineral absorption capacity. Copyright 2000 Annals of BotanyCompany Beta vulgaris L, carbon, nitrogen isotopic composition, rhizomania, seedling growth, seed reserves, sugarbeet.     

Isolation of polymorphic tetranucleotide microsatellite markers for the bay pipefish Syngnathus leptorhynchus

We have isolated nine polymorphic microsatellite markers for the bay pipefish Syngnathus leptorhynchus from genomic libraries enriched for (AAGG)_n repetitive elements. The number of alleles ranged from two to 15 per locus with the observed heterozygosity ranging from 0.09 to 1.00. These markers will be useful for analyses of questions concerning population genetic structure.     

Isolation of polymorphic tetranucleotide microsatellite markers for the black‐bellied seedcracker (Pyrenestes ostrinus)

We have isolated 10 polymorphic microsatellite markers for the black‐bellied seedcracker (Pyrenestes ostrinus) from genomic libraries enriched either for (AAGG)_n or (ATCT)_n repetitive elements and characterized them in 39 individuals. The number of alleles ranged from two to 27 per locus with the observed heterozygosity ranging from 0.38 to 0.94. These markers will be useful for analysis of questions concerning parentage and population genetic structure.