全文获取类型
收费全文 | 646篇 |
免费 | 76篇 |
出版年
2023年 | 4篇 |
2022年 | 6篇 |
2021年 | 22篇 |
2020年 | 10篇 |
2019年 | 9篇 |
2018年 | 19篇 |
2017年 | 18篇 |
2016年 | 21篇 |
2015年 | 35篇 |
2014年 | 42篇 |
2013年 | 37篇 |
2012年 | 50篇 |
2011年 | 40篇 |
2010年 | 31篇 |
2009年 | 34篇 |
2008年 | 35篇 |
2007年 | 35篇 |
2006年 | 39篇 |
2005年 | 37篇 |
2004年 | 26篇 |
2003年 | 23篇 |
2002年 | 24篇 |
2001年 | 7篇 |
2000年 | 7篇 |
1999年 | 12篇 |
1998年 | 8篇 |
1997年 | 3篇 |
1996年 | 8篇 |
1995年 | 7篇 |
1994年 | 3篇 |
1993年 | 3篇 |
1991年 | 6篇 |
1990年 | 9篇 |
1989年 | 3篇 |
1988年 | 3篇 |
1987年 | 4篇 |
1985年 | 3篇 |
1982年 | 2篇 |
1981年 | 3篇 |
1980年 | 3篇 |
1977年 | 2篇 |
1974年 | 3篇 |
1973年 | 2篇 |
1964年 | 2篇 |
1961年 | 3篇 |
1960年 | 2篇 |
1930年 | 1篇 |
1926年 | 1篇 |
1908年 | 1篇 |
1905年 | 1篇 |
排序方式: 共有722条查询结果,搜索用时 31 毫秒
1.
Jorge E. Ramos Gretta T. Pecl Natalie A. Moltschaniwskyj Jan M. Strugnell Rafael I. León Jayson M. Semmens 《PloS one》2014,9(8)
Understanding the response of any species to climate change can be challenging. However, in short-lived species the faster turnover of generations may facilitate the examination of responses associated with longer-term environmental change. Octopus tetricus, a commercially important species, has undergone a recent polewards range shift in the coastal waters of south-eastern Australia, thought to be associated with the southerly extension of the warm East Australian Current. At the cooler temperatures of a polewards distribution limit, growth of a species could be slower, potentially leading to a bigger body size and resulting in a slower population turnover, affecting population viability at the extreme of the distribution. Growth rates, body size, and life span of O. tetricus were examined at the leading edge of a polewards range shift in Tasmanian waters (40°S and 147°E) throughout 2011. Octopus tetricus had a relatively small body size and short lifespan of approximately 11 months that, despite cooler temperatures, would allow a high rate of population turnover and may facilitate the population increase necessary for successful establishment in the new extended area of the range. Temperature, food availability and gender appear to influence growth rate. Individuals that hatched during cooler and more productive conditions, but grew during warming conditions, exhibited faster growth rates and reached smaller body sizes than individuals that hatched into warmer waters but grew during cooling conditions. This study suggests that fast growth, small body size and associated rapid population turnover may facilitate the range shift of O. tetricus into Tasmanian waters. 相似文献
2.
Mike A. Steer Jayson M. Semmens 《Journal of experimental marine biology and ecology》2003,290(2):165-178
The influence of both predator and prey size on the shift from a pulling to a drilling predatory response was examined in the intertidal octopus Octopus dierythraeus, using an experimental program. Additionally, selective drilling, where particular regions of the prey are targeted, was examined for a variety of bivalve and gastropod prey. O. dierythraeus always initially attempted to pull bivalves apart. Shells that were eventually drilled were always subjected to significantly more pulling attempts than those that could be pulled apart, indicating that octopus are willing to expend more energy to access the flesh quickly. There was no defined threshold where bivalve size caused an octopus to switch from a pulling to a drilling response. Instead, there was a broad size range where the octopus could adopt either handling method and it varied for each individual. Octopus may only able to pull open bivalves before the molecular ratchet or ‘catch’ mechanism that many bivalves possess is engaged. This might explain the lack of a relationship between either octopus or bivalve size and the success of pulling, as it is likely that when the bivalves were presented to individual octopus they were either setting the ‘catch’ mechanism, or had already engaged it. O. dierythraeus demonstrated selective drilling on a variety of molluscan prey, with penetration sites differing between prey species. O. dierythraeus targeted the valve periphery, which was the thinnest part of the shell, therefore minimizing handling time. O. dierythraeus always drilled gastropods, but did not target the thinnest regions of the shells, with drill site varying according to the morphology of the prey. Elongate species with pronounced aperture lips were drilled in the apical region, close to the columella on the side of the opercula whereas nonelongate species were drilled immediately above the aperture. The location of drilling sites may represent a trade-off between targeting the most effective places to inject paralyzing secretions and the mechanically simplest places to drill. 相似文献
3.
G Stamatoyannopoulos P Constantoulakis M Brice S Kurachi T Papayannopoulou 《Developmental biology》1987,123(1):191-197
The cellular control of the switch from embryonic to fetal globin formation in man was investigated with studies of globin expression in erythroid cells of 35- to 56-day-old embryos. Analyses of globins synthesized in vivo and in cultures of erythroid progenitors (burst-forming units, BFUe) showed that cells of the yolk sac (primitive) erythropoiesis, in addition to embryonic chains, produced fetal and adult globins and that cells of the definitive (liver) erythropoiesis, in addition to fetal and adult globins, produce embryonic globins. That embryonic, fetal, and adult globins were coexpressed by cells of the same lineage was documented by analysis of globin chains in single BFUe colonies: all 67 yolk sac-origin BFUe colonies and 42 of 43 liver-origin BFUe colonies synthesized epsilon-, gamma-, and beta-chains. These data showed that during the switch from embryonic to adult globin formation, embryonic and definitive globin chains are coexpressed in the primitive, as well as in the definitive, erythroid cells. Such results are compatible with the postulate that the switch from embryonic to fetal globin synthesis represents a time-dependent change in programs of progenitor cells rather than a change in hemopoietic cell lineages. 相似文献
4.
J R López L E Brice?o G Cordovez V Sánchez N Linares 《General physiology and biophysics》1989,8(2):91-98
Carnitine is required for the transport of activated long chain fatty acids through the mitochondrial inner membrane. We measured the intracellular free calcium concentration [( Ca2+]i) by means of a calcium selective microelectrode in skeletal muscle biopsies obtained from nine patients in which myopathic carnitine deficiency (MCD) was diagnosed, and from six subjects with no evidence of neuromuscular disease. Intact intercostal muscle bundles were dissected and then split for electron microscopic studies and electrophysiological measurements. The [Ca2+]i in muscle fibers from MCD patients was 0.46 +/- 0.02 mumol.l-1 (mean +/- SEM) and 0.10 +/- 0.01 mumol.l-1 in control subjects. At the electron microscopic level, the predominant abnormality was the presence of lipid vacuoles between the myofibrils. These results show that in patients with myopathic carnitine deficiency there is a significant increase in the resting myoplasmic calcium concentration which might be related to a malfunction of some mechanisms responsible for the homeostasis of intracellular calcium. 相似文献
5.
6.
Rosalie J. Harris Callum Bryant Melinda A. Coleman Andrea Leigh Verónica F. Briceño Pieter A. Arnold Adrienne B. Nicotra 《Journal of phycology》2023,59(1):179-192
Foundation seaweed species are experiencing widespread declines and localized extinctions due to increased instability of sea surface temperature. Characterizing temperature thresholds are useful for predicting patterns of change and identifying species most vulnerable to extremes. Existing methods for characterizing seaweed thermal tolerance produce diverse metrics and are often time-consuming, making comparisons between species and techniques difficult, hindering insight into global patterns of change. Using three kelp species, we adapted a high-throughput method – previously used in terrestrial plant thermal biology – for use on kelps. This method employs temperature-dependent fluorescence (T–F0) curves under heating or cooling regimes to determine the critical temperature (Tcrit) of photosystem II (PSII), i.e., the breakpoint between slow and fast rise fluorescence response to changing temperature, enabling rapid assays of photosynthetic thermal tolerance using a standardized metric. This method enables characterization of Tcrit for up to 48 samples per two-hour assay, demonstrating the capacity of T–F0 curves for high-throughput assays of thermal tolerance. Temperature-dependent fluorescence curves and their derived metric, Tcrit, may offer a timely and powerful new method for the field of phycology, enabling characterization and comparison of photosynthetic thermal tolerance of seaweeds across many populations, species, and biomes. 相似文献
7.
Brice Maxime Nkouayeb Nangmou Hermine Laure Maza Djomkam Georges Bellier Tabekoueng Willifred Dongmo Tékapi Tsopgni Gabin Thierry Mbahbou Bitchagno Michel Arnaud Mbock Raceline Gounoue Kamkumo Marcel Frese Bruno Ndjakou Lenta Silvère Augustin Ngouela Nobert Sewald Anatole Guy Blaise Azebaze 《化学与生物多样性》2023,20(4):e202200271
The root extract of Nauclea xanthoxylon (A.Chev.) Aubrév. displayed significant 50 % inhibition concentration (IC50s) of 0.57 and 1.26 μg/mL against chloroquine resistant and sensitive Plasmodium falciparum (Pf) Dd2 and 3D7 strains, respectively. Bio-guided fractionation led to an ethyl acetate fraction with IC50s of 2.68 and 1.85 μg/mL and subsequently, to the new quinovic acid saponin named xanthoxyloside ( 1 ) with IC50s of 0.33 and 1.30 μM, respectively against the tested strains. Further compounds obtained from ethyl acetate and hexane fractions were the known clethric acid ( 2 ), ursolic acid ( 3 ), quafrinoic acid ( 4 ), quinovic acid ( 5 ), quinovic acid 3-O-β-D-fucopyranoside ( 6 ), oleanolic acid ( 7 ), oleanolic acid 3-acetate ( 8 ), friedelin ( 9 ), β-sitosterol ( 10a ), stigmasterol ( 10b ) and stigmasterol 3-O-β-D-glucopyranoside ( 11 ). Their structures were characterised with the aid of comprehensive spectroscopic methods (1 and 2D NMR, Mass). Bio-assays were performed using nucleic acid gel stain (SYBR green I)-based fluorescence assay with chloroquine as reference. Extracts and compounds exhibited good selectivity indices (SIs) of >10. Significant antiplasmodial activities measured for the crude extract, the ethyl acetate fraction and xanthoxyloside ( 1 ) from that fraction can justify the use of the root of N. xanthoxylon in ethnomedicine to treat malaria. 相似文献
8.
Isolation of Histoplasma capsulatum from an oil bird (steatornis caripensis) cave in Venezuela 总被引:2,自引:0,他引:2
Summary 1.Histoplasma capsulatum was recovered from two of fifteen soil specimens collected in a Venezuelan cave. This cave, located near Caripe in the state of Monagas, harbors a large colony of the oil bird,Steatornis caripensis.2. This finding confirms the previous discovery in Tingo Maria, Peru, of the association ofH. capsulatum with oil birds.3. The relationship ofH. capsulatum with animal habitats is discussed along with the public health importance of this association.
Zusammenfassung 1.Histoplasma capsulatum ist in zwei Bodenproben aus fünfzehn, die einer Höhle in Venezuela entnommen wurden, gefunden worden. Diese Höhle, die sich in der Nähe von Caripe, im Staate Monagas befindet, herbergt eine grosse Kolonie von Fettvögeln,Steatornis caripensis.2. Dieser Befund bestätigt die Vergesellschaftung vonH. capsulatum mit den Fettvögeln, wie es vorher bereits in Tingo Maria, Peru, entdeckt worden ist.3. Die Beziehung vonH. capsulatum zum tierischen Habitat wird diskutiert unter Betonung der Wichtigkeit dieser Vergesellschaftung vom Standpunkte des öffentlichen Gesundheitswesens.相似文献
9.
Nathalie Doerflinger Catherine Linder Karim Ouahchi Gabor Gyapay Jean Weissenbach Denis Le Paslier Philippe Rigault Samir Belal Christiane Ben Hamida Faycal Hentati Mongi Ben Hamida Massimo Pandolfo Stephano DiDonato Ronald Sokol Herbert Kayden Pierre Landrieu Alexandra Durr Alexis Brice Fran?oise Goutières Alfried Kohlschütter Pascal Sabouraud Ali Benomar Mohamed Yahyaoui Jean-Louis Mandel Michel Koenig 《American journal of human genetics》1995,56(5):1116-1124
Ataxia with vitamin E deficiency (AVED) is an autosomal recessive disease characterized clinically by neurological symptoms with often striking resemblance to those of Friedreich ataxia. This disorder has been reported previously as familial isolated vitamin E deficiency. We have mapped recently the AVED locus to a 5-cM confidence interval on chromosome 8q by homozygosity mapping in six Mediterranean families. We have now analyzed six new and two previously described families and demonstrate genetic homogeneity despite important clinical variability and wide geographic origins. Analysis of nine new tightly linked microsatellite markers, including four characterized in this study, revealed a predominant but not unique mutation in northern African populations, where this condition is more frequent. Haplotype analysis but also classical recombinations allowed us to refine the AVED position to a 1-cM interval. A YAC contig over this interval was constructed from marker STSs and YAC fingerprint data, in order to facilitate the search of the AVED gene. 相似文献