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1.
H. Bleckmann B. U. Budelmann T. H. Bullock 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1991,168(2):247-257
Potentials were recorded from the epidermal head lines and from the CNS of young cuttlefish, Sepia officinalis, in response to weak water movements. 1. Within the test range 0.5-400 Hz a sinusoidal water movement elicits up to 4 components of response if the electrode is placed on a headline: (i) a positive phasic ON response; (ii) a tonic frequency-following microphonic response; (iii) a slow negative OFF response; and (iv) compound nerve impulses. 2. The amplitude of both the ON wave and the microphonic potential depends on stimulus frequency, stimulus amplitude and stimulus rise time. Frequencies around 100 Hz and short rise times are most effective in eliciting strong potentials. The minimal threshold was 0.06 microns peak-to-peak water displacement at 100 Hz (18.8 microns/s as velocity). 3. Change of direction of tangential sphere movement (parallel vs. across the head lines) has only a small effect on the microphonic and the summed nerve potentials. 4. Frequency and/or amplitude modulations of a carrier stimulus elicit responses at the onset and offset of the modulation and marked changes in the tonic microphonic response. 5. Evoked potentials can be recorded from the brain while stimulating the epidermal lines with weak water movements. The brain potentials differ in several aspects from the potentials of the head lines and show little or no onset or offset wave at the transitions of a frequency and amplitude modulation. 相似文献
2.
Hardies SC; Martin SL; Voliva CF; Hutchison CA d; Edgell MH 《Molecular biology and evolution》1986,3(2):109-125
3.
R. G. Northcutt H. Bleckmann 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1993,172(4):439-446
The lateral line system of axolotls (Ambystoma mexicanum) consists of mechanoreceptive neuromasts and electroreceptive ampullary organs. All neuromasts in salamanders are located superficially and are organized into lines that are homologous to canal neuromasts in fishes. Ampullary organs are confined to the head and generally are located adjacent to the lines of superficial neuromasts. Axolotls, however, also possess a third class of receptors; these form restricted patches on the head and are possibly homologous to the superficial pit organs in fishes. In order to test this hypothesis the morphology of the suspected pit organs was examined with scanning electron microscopy, and a number of their physiological properties were determined. Pit organs are approximately half the size of neuromasts and have fewer hair cells, although these hair cells do possess kinocilia and stereocilia like those of neuromasts. Pit organs also possess cupulae and exhibit a pattern of innervation identical to that of neuromasts. Pit organs and neuromasts also exhibit similar rates of spontaneous activity, are excited by weak water currents but not weak electric stimuli, and are not inhibited by magnesium ions. Pit organs appear to have slightly lower rates of spontaneous discharge than neuromasts, however, and have slightly lower displacement thresholds to low frequency wave stimuli. These data support the contention that the pit organs of axolotls constitute a second class of neuromasts homologous to the pit organs of fishes. 相似文献
4.
H. Bleckmann R. Zelick 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1993,172(1):115-128
Mechanosensory lateral line afferents of weakly electric fish (Eigenmannia) responded to an object which moved parallel to the long axis of the fish with phases of increased spike activity separated by phases of below spontaneous activity. Responses increased with object speed but finally may show saturation. At increasingly greater distances the responses decayed as a power function of distance. For different object velocities the exponents (mean±SD) describing this response falloff were -0.71±0.4 (20 cm/s object velocity) and-1.9±1.25 (10 cm/s). Opposite directions of object movement may cause an inversion of the main features of the response histograms. In terms of peak spike rate or total number of spikes elicited, however, primary lateral line afferents were not directionally sensitive.Central (midbrain) lateral line units of weakly electric fish (Apteronotus) showed a jittery response if an object moved by. In midbrain mechanosensory lateral line, ampullary, and tuberous units the response to a rostral-tocaudal object movement may be different from that elicited by a caudal-to-rostral object motion. Central units of Apteronotus may receive input from two or more sensory modalities. Units may be lateral line-tuberous or lateral line-ampullary. Multimodal lateral line units were OR units, i.e., the units were reliably driven by a unimodal stimulus of either modality. The receptive fields of central units demonstrate a weak somatotopic organization of lateral line input: anterior body areas project to rostral midbrain, posterior body areas project to caudal midbrain.Abbreviation EOD
electric organ discharge 相似文献
5.
G. von der Emde H. Bleckmann 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1997,181(5):511-524
Electroreceptive afferents from A- and B-electroreceptor cells of mormyromasts and Knollenorgans were tested for their sensitivity
to different stimulus waveforms in the weakly electric fish Gnathonemus petersii. Both A- and B-mormyromast cells had their lowest sensitivity to a waveform similar to the self-generated electric organ
discharge (EOD) (around 0° phase-shift). Highest sensitivities, i.e. lowest response thresholds, in both A- and B-cells were
measured at phase shifts of +135°. Thus, both cell types were inversely waveform tuned. The sensitivity of B-cells increased
sharply with increasing waveform distortions. Their tuning curves had a sharp minimum of sensitivity at +7° phase shift. A-cells
had a much broader waveform tuning with a plateau level of low sensitivity from +24° to −15°. Across a 360° cycle of phase-shifts,
the range of thresholds was 16 dB for individual B-cells and 4.5 dB for individual A-cells. Knollenorgan afferents were tuned
to 0° phase-shifted EODs and had a dynamic range of 12 dB. Lowest sensitivities were measured at a phase shift of +165°. Experiments
with computer-generated stimuli revealed that the strong sensitivity of mormyromast B-cells of EOD waveform distortions cannot
be attributed to any of the seven waveform parameters tested. In addition, EOD stimuli must have the correct duration for
B-cells to respond to waveform distortions. Thus, waveform tuning appears to be based on the specific combination of several
waveform parameters that occur only with natural EODs.
Accepted: 28 April 1997 相似文献
6.
Adrian Klein Heinrich Münz Horst Bleckmann 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》2013,199(9):735-749
We investigated the filter properties of the highly branched trunk lateral lines of the stichaeid Xiphister atropurpureus and compared them to the filter properties of simple lateral line canals. For this purpose artificial canals were constructed, some of which were fitted with artificial neuromasts. In still water, the response of a simple canal versus two types of Xiphister-like canals to a vibrating sphere stimulus were similar, as was the decrease in the responses as a function of sphere distance. Also comparable was the mechanical coupling between neighboring parts of the main canal. However, compared to the simple canal, the Xiphister-like canals showed a lower spatial resolution. Equipping artificial lateral line canals with artificial neuromasts revealed that Xiphister-like canals, i.e., lateral lines canals with tubuli that contained widely spaced pores, improve the signal-to-noise ratio in a highly turbulent environment. Even though a reduced spatial resolution is the price for this improvement, Xiphister may compensate for this compromise by having four instead of the usual single trunk lateral line canal. We suggest that lateral line canals with tubuli that contain widely spaced pores and multiple lateral line canals on each body side are an adaptation to a highly turbulent aquatic environment. 相似文献
7.
Susanne Schwarze Horst Bleckmann Vera Schluessel 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》2013,199(10):843-856
Animals face different threats; to survive, they have to anticipate how to react or how to avoid these. It has already been shown in teleosts that selected regions in the telencephalon, i.e., the medial pallium, are involved in avoidance learning strategies. No such study exists for any chondrichthyan. In nature, an avoidance reaction may vary, ranging from a ‘freeze’ reaction to a startling response and quick escape. This study investigated whether elasmobranchs (Chiloscyllium griseum and C. punctatum) can be conditioned in an aversive classical conditioning paradigm. Upon successful conditioning, the dorsal, medial and lateral pallium were removed (group 1) and performance tested again. In a second group, the same operation was performed prior to training. While conditioning was successful in individuals of both groups, no escape responses were observed. Post-operative performance was assessed and compared between individual and groups to reveal if the neural substrates governing avoidance behavior or tasks learned in a classical conditioning paradigm are located within the telencephalon, as has been shown for teleosts such as goldfish. 相似文献
8.
Fast plasmid based protein expression analysis in insect cells using an automated SplitGFP screen 下载免费PDF全文
Maren Bleckmann Stefan Schmelz Christian Schinkowski Andrea Scrima Joop van den Heuvel 《Biotechnology and bioengineering》2016,113(9):1975-1983
Recombinant protein expression often presents a bottleneck for the production of proteins for use in many areas of animal‐cell biotechnology. Difficult‐to‐express proteins require the generation of numerous expression constructs, where popular prokaryotic screening systems often fail to identify expression of multi domain or full‐length protein constructs. Post‐translational modified mammalian proteins require an alternative host system such as insect cells using the Baculovirus Expression Vector System (BEVS). Unfortunately this is time‐, labor‐, and cost‐intensive. It is clearly desirable to find an automated and miniaturized fast multi‐sample screening method for protein expression in such systems. With this in mind, in this paper a high‐throughput initial expression screening method is described using an automated Microcultivation system in conjunction with fast plasmid based transient transfection in insect cells for the efficient generation of protein constructs. The applicability of the system is demonstrated for the difficult to express Nucleotide‐binding Oligomerization Domain‐containing protein 2 (NOD2). To enable detection of proper protein expression the rather weak plasmid based expression has been improved by a sensitive inline detection system. Here we present the functionality and application of the sensitive SplitGFP (split green fluorescent protein) detection system in insect cells. The successful expression of constructs is monitored by direct measurement of the fluorescence in the BioLector Microcultivation system. Additionally, we show that the results obtained with our plasmid‐based SplitGFP protein expression screen correlate directly to the level of soluble protein produced in BEVS. In conclusion our automated SplitGFP screen outlines a sensitive, fast and reliable method reducing the time and costs required for identifying the optimal expression construct prior to large scale protein production in baculovirus infected insect cells. Biotechnol. Bioeng. 2016;113: 1975–1983. © 2016 The Authors. Biotechnology and Bioengineering Published by Wiley Periodicals, Inc. 相似文献
9.
S.?Kr?ther H.?Bleckmann J.?MogdansEmail author 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》2004,190(6):437-448
We investigated how single units in the medial octavolateralis nucleus of the rainbow trout, Oncorhynchus mykiss, respond to a 50-Hz vibrating sphere in still and running water. Four types of units were distinguished. Type MI units (N=16) were flow-sensitive; their ongoing discharge rates either increased or decreased in running water, and as a consequence, responses of these units to the vibrating sphere were masked if the fish was exposed to water flow. Type MII units (N=7) were not flow-sensitive; their ongoing discharge rates were comparable in still and running water, and thus their responses to the vibrating sphere were not masked. Type MIII units (N=7) were also not flow-sensitive; nevertheless, their responses to the vibrating sphere were masked in running water. Type MIV units (N=14) were flow-sensitive, but their responses to the vibrating sphere were not masked. Our data confirm previous findings in the goldfish, Carassius auratus, indicating that the organization of the peripheral lateral line is reflected to a large degree in the medial octavolateralis nucleus. We compare data from goldfish and trout and discuss differences with respect to lateral line morphology, lifestyle and habitat of these species.Abbreviations CN canal neuromast - MON medial octavolateralis nucleus - SN superfical neuromast - a.c. alternating current - d.c. direct current 相似文献
10.