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The short-term and long-term effects of two most commonly used brown and black masheri were studied in Swiss mice and Syrian golden hamsters. In short-term studies, both the types of masheri extracts (ME) at 3/4 LD50 dose given ip did not have any effect on either liver or plasma vitamin C levels (both species). However, a decrease in liver vitamin A was observed only in hamsters injected with black ME. Similar effect was not observed in mice injected with both the types of masheri extracts. In long-term studies, when both the types of masheri were fed through diet at 10% level for 20 months, no effect was observed on hepatic or plasma vitamin C levels in mice (both sexes), while an increase in vitamin C levels was observed in black masheri diet fed hamsters. A depletion in liver vitamin A was observed in hamsters fed both the types of masheri. Such an effect was observed only in black masheri diet fed Swiss mice (both sexes) and brown masheri diet fed Swiss females. 相似文献
3.
The defatted starch was dispersed in NaOH (1 M) and neutralized with HCl (1 M). The amylose 1-butanol complex is adsorbed on defatted cellulose powder in the solvent system containing acetate buffer (pH 4.8,0.1 M) + urea (2 M) + 1-butanol (8.5%, v/v). The complex adsorbed on cellulose powder is separated by centrifugation (2418 g). The sediment is washed with the solvent system-I to obtain the intermediate fraction. The adsorbed amylose is eluted with urea (2 M) in acetate buffer (pH 4.8, 0.1 M). The amylose, intermediate fraction and amylopectin were precipitated with ethanol, washed free of urea and air dried. They were characterized by determining their blue value and beta -amylolysis limit. 相似文献
4.
Primary cultures of human fetal oesophageal cells were set up and maintained for 45 days. Epithelial cells were the dominant cell type in the culture for the first four weeks. Thereafter, both epithelial cells and fibroblasts were seen with the fibroblasts becoming the dominant cell type by the 6th week and until the cultures degenerated. The tritiated thymidine uptake showed an upward trend from day 10 up to day 30, with peak uptake at day 30 in the untreated, B(a)P treated and OAc treated cultures and decreased thereafter. The thymidine uptake levels were significantly higher in the B(a)P treated cultures when compared with levels in the untreated cultures. A concurrent increase/decrease was also seen in the cell number in all the three groups of cultures. Cultures with B(a)P and DMN-OAc showed significantly higher AHH levels as compared with untreated cultures. These results indicate that the human fetal oesophageal cells could be viably maintained under in vitro conditions for long periods of time and also showed capacity to metabolise the carcinogens through aryl hydrocarbon hydroxylase activity. 相似文献
5.
G V Bhide 《Steroids》1979,33(4):361-378
Asymmetric synthesis of D-homoestrone methyl ether was achieved by a new annalation reaction. 相似文献
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Arunkumar Venkatesan Jie Geng Malathi Kandarpa Sanjeeva Joseph Wijeyesakere Ashwini Bhide Moshe Talpaz Irina D. Pogozheva Malini Raghavan 《The Journal of cell biology》2021,220(7)
Myeloproliferative neoplasms (MPNs) are frequently driven by mutations within the C-terminal domain (C-domain) of calreticulin (CRT). CRTDel52 and CRTIns5 are recurrent mutations. Oncogenic transformation requires both mutated CRT and the thrombopoietin receptor (Mpl), but the molecular mechanism of CRT-mediated constitutive activation of Mpl is unknown. We show that the acquired C-domain of CRTDel52 mediates both Mpl binding and disulfide-linked CRTDel52 dimerization. Cysteine mutations within the novel C-domain (C400A and C404A) and the conserved N-terminal domain (N-domain; C163A) of CRTDel52 are required to reduce disulfide-mediated dimers and multimers of CRTDel52. Based on these data and published structures of CRT oligomers, we identify an N-domain dimerization interface relevant to both WT CRT and CRTDel52. Elimination of disulfide bonds and ionic interactions at both N-domain and C-domain dimerization interfaces is required to abrogate the ability of CRTDel52 to mediate cell proliferation via Mpl. Thus, MPNs exploit a natural dimerization interface of CRT combined with C-domain gain of function to achieve cell transformation. 相似文献
8.
Soong-Hoon Kim Keith L. Constantine Gerald J. Duke Valentina Goldfarb John T. Hunt Stephen Johnson Kevin Kish Herbert E. Klei Patricia A. McDonnell William J. Metzler Luciano Mueller Michael A. Poss Craig R. Fairchild Rajeev S. Bhide 《Bioorganic & medicinal chemistry letters》2013,23(14):4107-4111
The design, synthesis and characterization of a phosphonate inhibitor of N-acetylneuraminate-9-phosphate phosphatase (HDHD4) is described. Compound 3, where the substrate C-9 oxygen was replaced with a nonlabile CH2 group, inhibits HDHD4 with a binding affinity (IC50 11 μM) in the range of the native substrate Neu5Ac-9-P (compound 1, Km 47 μM). Combined SAR, modeling and NMR studies are consistent with the phosphonate group in inhibitor 3 forming a stable complex with native Mg2+. In addition to this key interaction, the C-1 carboxylate of the sugar interacts with a cluster of basic residues, K141, R104 and R72. Comparative NMR studies of compounds 3 and 1 with Ca2+ and Mg2+ are indicative of a highly dynamic process in the active site for the HDHD4/Mg2+/3 complex. Possible explanations for this observation are discussed. 相似文献
9.
Sepharose 4B affinity chromatography of Trichosanthes anguina seed extract and subsequent elution with galactose resulted in the isolation of an apparently single lectin with molecular weight of 45,000 +/- 700. However, major amount of the hemagglutinating activity was recovered as unadsorbed protein fraction. High affinity matrix Lactamyl Seralose could retain most of the galactose specific lectin activity from fraction 'A' which was eluted with lactose. It is evident from PAGE and SDS-PAGE analysis of the purified protein that T. anguina seeds contains a mixture of isolectins ranging in molecular weight from 30,000 to 50,000 +/- 1300. Periodic Acid Schiff's staining of the gels revealed this lectin complex to be a combination of glycosylated and non-glycosylated lectins. Two Isolectins SLc and IEL from within this complex have been isolated by affinity and ion exchange chromatography respectively. Apparent homology of these two lectins is indicated by their identical molecular weight (45 kDa), sub unit composition, non glycoprotein nature and immunological identity. However, these two lectins show minor differences in their biological and physicochemical properties. The peptide maps of the two lectins obtained after digestion with Trypsin and Pronase E also indicate minor changes in the primary structure. 相似文献
10.
Pradeep G. Bhide 《Seminars in cell & developmental biology》2009,20(4):395-402
Exposure of the developing fetus to cocaine produces lasting adverse effects on brain structure and function. Animal models show that cocaine exerts its effects by interfering with monoamine neurotransmitter function and that dopamine is cocaine's principal monoamine target in the fetal brain. This review will examine the role of dopamine receptor signaling in the regulation of normal development of the cerebral cortex, the seat of higher cognitive functions, and discuss whether dopamine receptor signaling mechanisms are the principal mediators of cocaine's deleterious effects on the ontogeny of cerebral cortical cytoarchitecture. 相似文献