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1.
Hobbie Erik Alan Bendiksen Katriina Thorp Nathan R. Ohenoja Esteri Ouimette Andrew P. 《Ecosystems》2022,25(5):1207-1217
Ecosystems - A major functional division in ectomycorrhizal fungi is between taxa with hydrophobic ectomycorrhizae (strong proteolytic capabilities, deep nitrogen (N) acquisition, and extensive... 相似文献
2.
Ringø E Bendiksen HR Wesmajervi MS Olsen RE Jansen PA Mikkelsen H 《Journal of applied microbiology》2000,89(2):317-322
The present study reports the effect of excessive handling stress and starvation on the lactic acid bacteria associated with the digestive tract of Atlantic salmon (Salmo salar L.). A relatively low population level (approximately 2 x 103 bacteria per gram wet tissue) of viable adherent heterotrophic bacteria was associated with the digestive tract (foregut, midgut and hindgut). Of the 752 bacterial isolates isolated from diet, water and the digestive tract, 201 isolates belonged to the carnobacteria. Of these isolates, one from the diet, one from the rearing water and 80 from the gastrointestinal tract, were further identified on the basis of 16S rDNA sequence analysis. All these isolates were identified as being Carnobacterium piscicola-like. Daily repeated stress and starvation of the fish over 11 d had no influence on the total culturable bacterial numbers or population level of C. piscicola associated with the digestive tract. C. piscicola-like isolates colonizing the various intestinal regions (foregut, midgut and hindgut) were also screened for their ability to produce growth inhibitory compounds active against the fish pathogen Aeromonas salmonicida. Of the 199 C. piscicola isolates tested, 139 inhibited growth of the pathogen. 相似文献
3.
Anders N. Hansen Christine D. Bendiksen Lene Sylvest Tina Friis Dan Staerk Flemming Steen J?rgensen Christian A. Olsen Gunnar Houen 《PloS one》2012,7(9)
Inhibition of angiogenesis is a promising addition to current cancer treatment strategies. Neutralization of vascular endothelial growth factor by monoclonal antibodies is clinically effective but may cause side effects due to thrombosis. Low molecular weight angiogenesis inhibitors are currently less effective than antibody treatment and are also associated with serious side effects. The discovery of new chemotypes with efficient antiangiogenic activity is therefore of pertinent interest. (S)-Levamisole hydrochloride, an anthelminthic drug approved for human use and with a known clinical profile, was recently shown to be an inhibitor of angiogenesis in vitro and exhibited tumor growth inhibition in mice. Here we describe the synthesis and in vitro evaluation of a series of N-alkylated analogues of levamisole with the aim of characterizing structure–activity relationships with regard to inhibition of angiogenesis. N-Methyllevamisole and p-bromolevamisole proved more effective than the parent compound, (S)-levamisole hydrochloride, with respect to inhibition of angiogenesis and induction of undifferentiated cluster morphology in human umbilical vein endothelial cells grown in co-culture with normal human dermal fibroblasts. Interestingly, the cluster morphology caused by N-methyllevamisole was different than the clusters observed for levamisole, and a third “cord-like” morphology resembling that of the known drug suramin was observed for an aniline-containing derivative. New chemotypes exhibiting antiangiogenic effects in vitro are thus described, and further investigation of their underlying mechanism of action is warranted. 相似文献
4.
Berg V Sveinbjörnsson B Bendiksen S Brox J Meknas K Figenschau Y 《Arthritis research & therapy》2010,12(6):R228
Introduction
Chemerin is a chemotactic peptide which directs leukocytes expressing the chemokine-like receptor ChemR23 towards sites of inflammation. ChemR23 is a G protein-coupled receptor which binds several different ligands, and it is also expressed by other cell types such as adipocytes. In addition to chemotaxis, recent reports suggest that ChemR23 is capable of mediating either inflammatory or anti-inflammatory effects, depending on the type of ligand it binds. In the present study, we aimed to clarify whether human chondrocytes express ChemR23 and chemerin, and whether chemerin/ChemR23 signalling could affect secretion of inflammatory mediators. 相似文献5.
Ringø E Wesmajervi MS Bendiksen HR Berg A Olsen RE Johnsen T Mikkelsen H Seppola M Strøm E Holzapfel W 《Systematic and applied microbiology》2001,24(2):183-191
Eleven bacterial strains were isolated from the gastrointestinal tract of four fish species, Atlantic salmon (Salmo salar L.), Arctic charr (Salvelinus alpinus L.), Atlantic cod (Gadus morhua L.) and wolffish (Anarhichas lupus L.). All the strains were Gram-positive rods, non-sporing, catalase and oxidase-negative, able to grow at pH 9.0 but not on acetate containing media (pH < or = 5.4), and were fermentative. They had a high content of oleic acid (18:1 n-9) in cellular lipid, and were found to belong to the genus Carnobacterium by phenotypic criteria. The eleven carnobacteria strains were further identified on the basis of 16S rDNA sequence analysis and AFLP(TM) fingerprinting. 相似文献
6.
Benjamin A. Sandkam Jeffrey B. Joy Corey T. Watson Pablo Gonzalez‐Bendiksen Caitlin R. Gabor Felix Breden 《Evolution; international journal of organic evolution》2013,67(1):120-130
Expansions in sensory systems usually require processes such as gene duplication and divergence, and thus evolve slowly. We evaluate a novel mechanism leading to rapid sensory repertoire expansion: hybrid‐sensory expansion (HSE). HSE occurs when two species with differently tuned sensory systems form a hybrid, bringing together alleles from each of the parental species. In one generation, a sensory repertoire is created that is the sum of the variance between parental species. The Amazon molly presents a unique opportunity to test the HSE hypothesis in a “frozen” hybrid. We compared opsin sequences of the Amazon molly, Poecilia formosa, to those of the parental species. Both parental species are homozygous at the RH2–1 locus and each of the four long wavelength sensitive loci, while P. formosa possess two different alleles at these loci; one matching each parental allele. Gene expression analysis showed P. formosa use the expanded opsin repertoire that was the result of HSE. Additionally, behavioral tests revealed P. formosa respond to colored stimuli in a manner similar or intermediate to the parental species P. mexicana and P. latipinna. Together these results strongly support the HSE hypothesis. Hybrid‐sensory repertoire expansion is likely important in other hybrid species and in other sensory systems. 相似文献
7.
Bendiksen S Van Ghelue M Winkler T Moens U Rekvig OP 《Journal of immunology (Baltimore, Md. : 1950)》2004,173(12):7630-7640
The mechanism(s) responsible for autoimmunity to DNA and nucleosomes in SLE is largely unknown. We have demonstrated that nucleosome-polyomavirus T-Ag complexes, formed in context of productive polyomavirus infection, activate dsDNA-specific B cells and nucleosome-specific CD4(+) T cells. To investigate whether de novo expressed T-Ag is able to terminate nucleosome-specific T cell tolerance and to maintain anti-dsDNA Ab production in nonautoimmune mice, we developed two binary transgenic mouse variants in which expression of SV40 large T-Ag is controlled by tetracycline, MUP tTA/T-Ag (tet-off), and CMV rtTA/T-Ag (tet-on) mice. Data demonstrate that MUP tTA/T-Ag mice, but not CMV rtTA/T-Ag mice, are tightly controlling T-Ag expression. In MUP tTA/T-Ag transgenic mice, postnatal T-Ag expression activated CD8(+) T cells but not DNA-specific B cells, while immunization with T-Ag and nucleosome-T-Ag-complexes before T-Ag expression resulted in elevated and remarkably stable titers of anti-T-Ag and anti-dsDNA Abs and activation of T-Ag-specific CD4(+) T cells. Immunization of nonexpressing MUP tTA/T-Ag mice resulted in transient anti-T-Ag and anti-dsDNA Abs. This system reveals that a de novo expressed DNA-binding quasi-autoantigen maintain anti-dsDNA Abs and CD4(+) T cell activation once initiated by immunization, demonstrating direct impact of a single in vivo expressed molecule on sustained autoimmunity to DNA and nucleosomes. 相似文献
8.
A survey of the patterns of lignicolous fungi to record occurrence of fruitbodies on fallen logs of Picea abies . has been performed. Ninetythree forest stands with different ages. ecology, and histories of management in Ser-Trendelag County, Central Norway were investigated. In each stand an investigation plot of 40 m diameter was established, in which five logs were randomly selected. Environmental variables of the logs and plots and geographical positions of the plots were recorded. The numerical methods used include detrended correspondence analysis (DCA) and canonical correspondence analysis (CCA).
A total of 140 species of Aphyllophorales, Agaricales with eccentrically attached caps, heterobasiodomycetes, and prominent ascomycetes and deuteromycetes were registered. More than one third of the species were only found once. The main gradient in the data set was shown to be related to decay. The second gradient was related to size of the logs and some forest management variables (in addition to decay). Of the variation in composition of the species, 13.8 % can be ascribed to solely environmental variance, 6 % to solely spatial structure, and only 0.8 % to the combination of the environment and spatial component of variation. The results are discussed in the light of wood decay, forest management, and landscape fragmentation. 相似文献
A total of 140 species of Aphyllophorales, Agaricales with eccentrically attached caps, heterobasiodomycetes, and prominent ascomycetes and deuteromycetes were registered. More than one third of the species were only found once. The main gradient in the data set was shown to be related to decay. The second gradient was related to size of the logs and some forest management variables (in addition to decay). Of the variation in composition of the species, 13.8 % can be ascribed to solely environmental variance, 6 % to solely spatial structure, and only 0.8 % to the combination of the environment and spatial component of variation. The results are discussed in the light of wood decay, forest management, and landscape fragmentation. 相似文献
9.
Erik A. Hobbie Andrew P. Ouimette Edward A. G. Schuur Daniel Kierstead James M. Trappe Katriina Bendiksen Esteri Ohenoja 《Biogeochemistry》2013,114(1-3):381-389
Organic nitrogen use by mycorrhizal fungi and associated plants could fuel productivity in nitrogen-limited systems. To test whether fungi assimilated soil-derived organic nitrogen, we compared the 14C signal (expressed as Δ14C) from 1950s to 1960s thermonuclear testing in protein and structural carbon of ectomycorrhizal fungi. As expected, structural carbon had Δ14C similar to recent photosynthesis; however, protein Δ14C was either higher or lower than structural carbon depending on the fungal taxa. This suggests that some protein carbon derived from uptake of organic nitrogen with different Δ14C signals. Specimens from two taxa (Lactarius and Russula) adapted to taking up soluble nutrients had protein higher than structural carbon in Δ14C, indicating uptake of young, post-bomb organic nitrogen, whereas two taxa (Cortinarius and Leccinum) adapted to using insoluble, complex organic nutrients had protein lower than structural carbon in Δ14C, indicating uptake of old, pre-bomb organic nitrogen. Tuber, a genus common in mineral soil, was also consistently lower in Δ14C for protein than for structural carbon, with an estimated 10 % of protein carbon originating from old, deep organic nitrogen for this taxon. Our results indicate that radiocarbon can provide evidence of organic nitrogen use in ectomycorrhizal fungi and reflects the exploration depth of different taxa. 相似文献
10.
Design and test of a grid to reduce bycatch in the longfin inshore squid (Doryteuthis pealeii) trawl fishery 下载免费PDF全文
S. M. Bayse M. V. Pol M. Walsh A. Walsh T. Bendiksen P. He 《Zeitschrift fur angewandte Ichthyologie》2017,33(5):898-906
A species separation grid was tested for a squid trawl to reduce finfish bycatch in the Nantucket Sound longfin inshore squid (Doryteuthis pealeii) fishery in southern New England, USA. The experimental trawl with a grid significantly reduces bycatch of summer flounder (Paralichthys dentatus) (76.4%, p < .001), black sea bass (Centropristis striata) (71.7%, p = .001), smooth dogfish (Mustelus canis) (86.0%, p < .001), and total bycatch (69.2%, p < .001) when compared to a conventional trawl. The catch rate of scup (Stenotomus chrysops) is 40.2% less than in the experimental trawl, but this difference is not statistically significant (p = .258). However, the experimental trawl also reduces targeted squid capture by 47.5% (p < .001), which is commercially unacceptable. Length analysis indicates no size effect on the retention for squid between the trawl with a grid (experimental) and the one without a grid (control), but the experimental trawl significantly reduces larger scup (>27 cm FL) and larger black sea bass (>37 cm TL), and all summer flounder size‐classes. Therefore, this grid design may not be a suitable bycatch reduction device for the Nantucket Sound squid trawl fishery, and further work is needed to understand squid behavior within a trawl to develop a successful bycatch reduction strategy for the New England longfin inshore squid fishery. 相似文献