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Sharma Shivani Kumar Amit Dhakte Priyanka Raturi Gaurav Vishwakarma Gautam Barbadikar Kalyani M. Das B. K. Shivaraj S. M. Sonah Humira Deshmukh Rupesh 《Journal of Plant Growth Regulation》2023,42(1):46-59
Journal of Plant Growth Regulation - Crop improvement in light of the rapidly changing climate and the increasing human population continues to be one of the primary concerns for researchers across... 相似文献
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Reema Rani Prashant Yadav Kalyani M. Barbadikar Nikita Baliyan Era Vaidya Malhotra Binay Kumar Singh Arun Kumar Dhiraj Singh 《Biotechnology letters》2016,38(12):1991-2006
Creation of variation in existing gene pool of crop plants is the foremost requirement in crop improvement programmes. Genome editing is a tool to produce knock out of target genes either by introduction of insertion or by deletion that disrupts the function of a specific gene. The CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9) system is the most recent addition to the toolbox of sequence-specific nucleases that includes ZFNs and TALENs. The CRISPR/Cas9 system allows targeted cleavage of genomic DNA guided by a small noncoding RNA, resulting in gene modifications by both non-homologous end joining and homology-directed repair mechanisms. Here, we present an overview of mechanisms of CRISPR, its potential roles in creating variation in germplasm and applications of this novel interference pathway in crop improvement. The availability of the CRISPR/Cas9 system holds promise in facilitating both forward and reverse genetics and will enhance research in crops that lack genetic resources. 相似文献
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