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ANDRÉ PICARD JEAN-CLAUDE LABBÉ GÉRARD PEAUCELLIER FRANÇOISE LE BOUFFANT CHRISTIAN LE PEUCH MARCEL DOREE 《Development, growth & differentiation》1987,29(1):93-103
Many studies suggest that MPF activation depends on protein phosphorylation or that MPF is itself a protein kinase. In the present report, cyclic variations of MPF activity have been correlated in vivo with changes in the extent of protein phosphorylation or in vitro with changes of a major protein kinase during the first cell cycles of fertilized starfish eggs. This cycling protein kinase neither requires cAMP nor Ca2+ . Neither colchicine nor aphidicoline, which inhibits cleavage and chromosome replication respectively, was found to suppress the synchronous and cyclic variations of both MPF and protein kinase activities. Protein synthesis was found to be required for both MPF and protein kinase activities to reappear after their simultaneous drop at the time of mitotic or meiotic cleavages. Production of either MPF or protein kinase activities is not the immediate result of protein synthesis since there is a delay at each cell cycle between the time when protein synthesis is required and the time when both MPF and protein kinase activities are produced. This suggests that both MPF and protein kinase activities might involve some post-translational modification of a precursor protein synthesized during the preceeding cell cycle. 相似文献
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ANDRÉ PICARD GÉRARD PEAUCELLIER FRANCOISE LE BOUFFANT MARCEL DOREE 《Development, growth & differentiation》1985,27(3):251-262
Single nuclei (germinal vesicles) manually isolated from large oocytes of the starfish Echinaster sepositus , as well as the complementary anucleated oocytes, were used to investigate the early changes of protein phosphorylation which occur from 1-MeAde addition to germinal vesicle breakdown (GVBD). Stimulation of protein phosphorylation was already evident in the nucleus shortly after 1-MeAde addition (18 min, thus about 0.40x the time required for GVBD), although it began first in the cytoplasm. No translocation of phosphoprotein across the nuclear envelope was detected before GVBD. Presence of the nucleus is not required for the hormone to stimulate protein phosphorylation in the remaining part of the oocytetin:fact the patterns of protein phosphorylation in enucleated oocytes were found to be identical, whether enucleation was performed after or before hormonal treatment. Cytoplasm taken at the time of GVBD from maturing Echinaster oocytes induces meiotic maturation when transferred in stage VI immature oocytes of the amphibian Xenopus laevis. 相似文献
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