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1.
R Calderone E Wadsworth 《Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)》1987,185(3):325-334
Antigenic differences between a wild-type virulent Candida albicans 4918 (wt) and its spontaneous avirulent mutant (m-10) were found with crossed immunoelectrophoresis. Yeast cell extracts as well as soluble protein and mannoprotein fractions obtained by affinity chromatography on concanavalin A (Con A) were analyzed. Sera from patients with candidiasis and antisera from rabbits infected with live wt cells and boosted with wt extracts or rabbits immunized with purified wt cell wall preparation were used as counter reactants. Qualitative differences in serum precipitins formed by patients with suspected or culture-proven candidiasis to polysaccharide antigens of wt and m-10 origin were observed. In comparison, except for a spike-formed precipitate detected only with the wt extract, the serum from infected rabbits precipitated the wt and m-10 cell wall polysaccharide antigens about equally. The same type of precipitate was also found with the Con A wt mannoprotein fractions but was again lacking with the m-10 mannoproteins. This precipitate, with extremely slow electromobility in the first dimension, may be related to some special immunodeterminant of the wt mannan molecule. No substantial differences in the precipitation patterns of the Con A wt and m-10 proteins were found when analyzed with patients' sera or rabbit anti-cell sera. However, using these protein fractions with anti-cell wall sera revealed a larger number of precipitates for the wt as opposed to the m-10 strain. The observed antigenic differences between the virulent- and the avirulent-derived strains seem to be mainly associated with cell wall determinants (components) and might be related to the greater adherence and infectivity of the wild strain. 相似文献
2.
Sterol carrier protein2-like activity in rat intestine 总被引:1,自引:0,他引:1
A Kharroubi J A Wadsworth R Chanderbhan P Wiesenfeld B Noland T Scallen G V Vahouny L L Gallo 《Journal of lipid research》1988,29(3):287-292
A sterol carrier protein2 (SCP2)-like activity has been demonstrated in rat intestinal mucosal homogenates and in isolated intestinal cells from both crypt and villus zones. The results indicate the presence of a protein with similar molecular weight and antigenicity to that of authentic SCP2 purified from rat liver cytosol. Like liver SCP2, mucosal cytosol stimulates pregnenolone production in rat adrenal mitochondria and acyl coenzyme A:cholesterol acyltransferase activity of liver and mucosal microsomes. The distribution of SCP2-like activity as determined by radioimmunoassay indicates high levels in mitochondria and cytosol and relatively lower levels in microsomes and in brush-border membranes. The widespread distribution of SCP2-like protein in the intestine is consistent with potential transfer functions in all phases of cholesterol processing. 相似文献
3.
H L Wadsworth D Russo Y Nagayama G D Chazenbalk B Rapoport 《Molecular endocrinology (Baltimore, Md.)》1992,6(3):394-398
We previously reported that deletion or substitution of a unique eight-amino acid tract (residues 38-45) in the extracellular domain of the human TSH receptor led to the loss of specific ligand binding to the surface of transfected cells. In the present study we analyzed this region in more detail. Using site-directed mutagenesis of the TSH receptor cDNA, we substituted amino acid residues 38-45, either in three overlapping groups of four amino acids each or individually. The resultant TSH receptor mutant cDNAs were stably transfected into Chinese hamster ovary cells, and the cells were tested for their TSH-binding ability. Our data demonstrate that amino acid residues 38-40 and 42-45 in this region of the human TSH receptor can be substituted without alteration in receptor function and are, therefore, not critical in forming or maintaining the TSH-binding site. However, substitution of Cys41, either alone or together with adjacent amino acids, leads to the loss of TSH binding to its receptor. These data suggest a central role for the amino acid in position 41 in preserving the biological function of the TSH receptor. 相似文献
4.
Analysis of the treadmilling model during metaphase of mitosis using fluorescence redistribution after photobleaching 总被引:23,自引:19,他引:4 下载免费PDF全文
One recent hypothesis for the mechanism of chromosome movement during mitosis predicts that a continual, uniform, poleward flow or "treadmilling" of microtubules occurs within the half-spindle between the chromosomes and the poles during mitosis (Margolis, R. L., and L. Wilson, 1981, Nature (Lond.), 293:705-711). We have tested this treadmilling hypothesis using fluorescent analog cytochemistry and measurements of fluorescence redistribution after photobleaching to examine microtubule behavior during metaphase of mitosis. Mitotic BSC 1 mammalian tissue culture cells or newt lung epithelial cells were microinjected with brain tubulin labeled with 5-(4,6-dichlorotriazin-2-yl) amino fluorescein (DTAF) to provide a fluorescent tracer of the endogenous tubulin pool. Using a laser microbeam, fluorescence in the half-spindle was photobleached in either a narrow 1.6 micron wide bar pattern across the half-spingle or in a circular area of 2.8 or 4.5 micron diameter. Fluorescence recovery in the spindle fibers, measured using video microscopy or photometric techniques, occurs as bleached DTAF-tubulin subunits within the microtubules are exchanged for unbleached DTAF-tubulin in the cytosol by steady-state microtubule assembly-disassembly pathways. Recovery of 75% of the bleached fluorescence follows first-order kinetics and has an average half-time of 37 sec, at 31-33 degrees C. No translocation of the bleached bar region could be detected during fluorescence recovery, and the rate of recovery was independent of the size of the bleached spot. These results reveal that, for 75% of the half-spindle microtubules, FRAP does not occur by a synchronous treadmilling mechanism. 相似文献
5.
M E Wadsworth H A Cripps R E Midwinter J R Colley 《BMJ (Clinical research ed.)》1985,291(6508):1534-1538
Blood pressure was measured in a birth cohort of 5362 subjects at the age of 36. The prevalence of hypertension in men (blood pressure greater than 140/90 mm Hg) was almost twice that in women, although women received treatment more often. Deaths of fathers of subjects from hypertensive and ischaemic heart disease were associated with significantly higher mean systolic and diastolic pressures in both sexes. Cigarette smoking was not strongly associated with blood pressure in men and not associated at all in women. Of the social factors, low social class of family of origin was associated with high blood pressure in both sexes; but the strongest association was with current body mass, and birth weight also contributed. Differences in blood pressures between the sexes may have been related to protective biological factors, such as endogenous sex hormones, in women and also to differences in types of employment, smoking habits, and body mass. Differences in blood pressures related to the social class of family of origin may reflect long term influences of class differences on diet, exercise, and educational achievement. The importance of measuring secular trends in obesity and blood pressures is emphasised. 相似文献
6.
Early mouse embryos produce and release factors with transforming growth factor activity 总被引:2,自引:0,他引:2
Angie Rizzino 《In vitro cellular & developmental biology. Plant》1985,21(9):531-536
Summary Previous studies have shown that extracts from mouse embryos at mid and late stages of development contain factors that exhibit
transforming growth factor activity. The work reported here demonstrates that cultured mouse embryos at significantly earlier
stages of development produce and release factors that exhibit the characteristic property of transforming growth factors.
Specifically, the data demonstrate that embryos cultured from the blastocyst stage in serum-containing medium or in serum-free
medium release factors that promote the anchorage-independent growth of normal rat kidney fibroblasts. It is shown that these
factors are produced and released by cells derived from the inner cell mass and by trophoblasts. The precise developmental
stage when production of these factors first begins has not been determined but our findings suggest that these factors are
produced by cell types associated with early postimplatation embryos.
This work was supported by the Laboratory of Viral Carcinogenesis at the National Cancer Institute and by grants from the
National Cancer Institute (CA-36727) and the University of Nebraska Medical Center (22-271-732).
Editor's Statement This paper presents evidence that, in an in vitro assay system, early embryonic cells are capable of both
synthesizing and secreting TGF-like growth factors, implicating the production of these factors in the events of early development.
David W. Barnes 相似文献
7.
John Geigert Demetrios J. Dalietos Saul L. Neidleman Terry D. Lee Julie Wadsworth 《Biochemical and biophysical research communications》1983,114(3):1104-1108
Chloroperoxidase catalyzes the peroxidation of primary alcohols, specifically those that are allylic, propargylic, or benzylic. Aldehydes are the products. The reaction dislays appreciable activity throughout the entire pH range investigated, namely pH 3.0–7.0. This enzyme is the only haloperoxidase of four tested capable of carrying out the reaction. These results further establish chloroperoxidase as a unique haloperoxidase. 相似文献
8.
9.
Angie Rizzino Peter Kazakoff John Nebelsick 《In vitro cellular & developmental biology. Plant》1990,26(5):537-542
Summary Previous studies have shown that cell density can regulate the binding of several growth factors. To determine whether cell
density exerts a uniform effect on the expression of epidermal growth factor (EGF) receptors, seven cell lines were examined
in detail. For each cell line, EGF binding was found to decrease as cell density increases. Scatchard analysis of the binding
data reveals that decreases in EGF binding are due to reductions in the number of cell surface EGF receptors. The only apparent
exception is the effect of cell density on the binding of EGF to A-431 cells. For these cells, increases in cell density lead
to two effects: decreases in the number of high affinity EGF receptors and increases in the total number of EGF receptors.
In addition to the effects of cell density on EGF receptors, it was determined that increases in cell density can coordinately
down-regulate receptors for as many as four different growth factors. Overall, the findings described in this report for EGF
and those previously described for transforming growth factor type-β (TGF-β) and fibroblast growth factor (FGF) demonstrate
the existence of a common mechanism for down-regulating growth factor receptors.
This work was supported by grants from the Nebraska Department of Health (89-51), the National Cancer Institute (Laboratory
Research Center Support Grant, CA36727), and the American Cancer Society (Core Grant ACS SIG-16).
EDITOR'S STATEMENT The paper by Rizzino et al. demonstrates that receptor number decreases as a function of cell density.
This may represent a mechanism by which cell proliferation is reduced as cell density increases. 相似文献
10.