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The binding of isolated high mobility group proteins HMG (1+2) with nucleosomes was studied using gel electrophoresis. The interaction of HMG (1+2) with mononucleosomes could be detected as a new discrete electrophoretic band with a decreased mobility only after cross-linking of HMG (1+2)-nucleosome complex by formaldehyde. Approximately two molecules of the large HMG proteins were bound per nucleosomal particle of a DNA length of 185 base pairs, lacking histones H1 and H5. Using the same techniques, no binding was observed with core particles of a DNA length of 145 base pairs. 相似文献
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Dr. Andrei V. Dronov 《Facies》1993,28(1):169-180
Summary Several Waulsortian-type mud mounds nearly 500 m thick and about 5 km long occur in the Middle Paleozoic carbonate section
of the Aktur nappe in the mountains on the right bank of Isfara river. These buildups form a well developed barrier system
that stretches along the South Ferganian carbonate platform margin and divides the carbonate complex into a fore-reef and
a back-reef part. The time of the mounds' most active growth was from the Late Silurian (Ludlow) to the Middle Devonian (Eifel).
Three main facies types can be recognized in the mud mounds: 1. micritic core facies, 2. sparitic flank facies and 3. loferitic
capping facies. The central massive or crudely bedded part of the mounds consists of white or light grey clotted micrite.
Macrofossils are rare. The sparitic flank facies in contrast consists of coarse and densely packed crinoidal wackestone-floatstones
with some brachiopod shell debris. Solitary rugose corals, tabulate corals, stromato-poroids and fragments of mollusks are
also abundant. The tops of the mounds are usually covered with loferitic pelmicrites or oolitic grainstone caps. Stromatactis-like
structures are very rare and poorly developed in the South Ferganian mud mounds. However, almostin all such mounds horizons
of calcitic breccias can be found.
In order to explain all the features found in the Fergana mounds an ‘atoll-like’ model has been proposed which starts the
evolution of the mud mounds with a small nucleus bioherm. The main stage of the evolution corresponds to an atoll-like structure
developing on the surface of shallow water platforms. White clotted micrite of the mound core facies is interpreted as a accumulation
of fine-grained sediment in an inner lagoon flanked by crinoidal bar deposits. The mound flank facies represents the atoll
rim deposits from where the carbonate mud is derived. The capping loferitic facies is considered as tidal flat deposit that
developed on top of the buildups during the last stage of its evolution. The knoll shape of the mounds is explained by the
retreat of the atoll flanking crinoidal bars back into the inner lagoon during the rise in sea level. Stromatactis-like structures
of small cavities filled with sparry calcite owe their existence to burrowing organisms. Calcitic breccias are interpreted
as paleokarst collapse breccias. They indicate that the tops of the mud mound became subaerially exposed. Other evidence for
a subaerial exposure can be seen in the occurrence of Variscian ‘black and white’ limestone gravel on the tops of some mud
mounds. According toWard et al. (1970) these sediments were produced above the sea level at the edge of hypersaline lakes situated on islands. 相似文献
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Andrei O. Zalensky John W. Breneman Irina A. Zalenskaya B. R. Brinkley E. Morton Bradbury 《Chromosoma》1993,102(8):509-518
The localization of centromeres in mature human sperm was shown by immunofluorescent labeling and nonisotopic in situ hybridization. In the decondensed nucleus structural elements (dimers, tetramers, linear arrays and V shape structures) formed by individual centromeres of nonhomologous chromosomes were observed. They organize the compact chromocenter, which was shown for nuclei decondensed to a low extent. The chromocenter is buried inside the nucleus; in contrast, telomeric regions of chromosomes were tentatively localized on the periphery. Thus, a gross architecture, which can influence selective unpackaging of the paternal genome upon fertilization, exists in human sperm. 相似文献
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Anastassia M. Makarieva Andrei V. Nefiodov Antonio Donato Nobre Mara Baudena Ugo Bardi Douglas Sheil Scott R. Saleska Ruben D. Molina Anja Rammig 《Global Change Biology》2023,29(9):2536-2556
The terrestrial water cycle links the soil and atmosphere moisture reservoirs through four fluxes: precipitation, evaporation, runoff, and atmospheric moisture convergence (net import of water vapor to balance runoff). Each of these processes is essential for sustaining human and ecosystem well-being. Predicting how the water cycle responds to changes in vegetation cover remains a challenge. Recently, changes in plant transpiration across the Amazon basin were shown to be associated disproportionately with changes in rainfall, suggesting that even small declines in transpiration (e.g., from deforestation) would lead to much larger declines in rainfall. Here, constraining these findings by the law of mass conservation, we show that in a sufficiently wet atmosphere, forest transpiration can control atmospheric moisture convergence such that increased transpiration enhances atmospheric moisture import and results in water yield. Conversely, in a sufficiently dry atmosphere increased transpiration reduces atmospheric moisture convergence and water yield. This previously unrecognized dichotomy can explain the otherwise mixed observations of how water yield responds to re-greening, as we illustrate with examples from China's Loess Plateau. Our analysis indicates that any additional precipitation recycling due to additional vegetation increases precipitation but decreases local water yield and steady-state runoff. Therefore, in the drier regions/periods and early stages of ecological restoration, the role of vegetation can be confined to precipitation recycling, while once a wetter stage is achieved, additional vegetation enhances atmospheric moisture convergence and water yield. Recent analyses indicate that the latter regime dominates the global response of the terrestrial water cycle to re-greening. Evaluating the transition between regimes, and recognizing the potential of vegetation for enhancing moisture convergence, are crucial for characterizing the consequences of deforestation as well as for motivating and guiding ecological restoration. 相似文献