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Anjos Marcia S. Bitencourt Jamille A. Nunes Lorena A. Sarmento-Soares Luisa M. Carvalho Daniel C. Armbruster Jonathan W. Affonso Paulo R. A. M. 《Hydrobiologia》2020,847(2):563-578
Hydrobiologia - Integrative approaches are particularly useful to resolve taxonomic uncertainties in species-rich groups that have undergone explosive radiation, such as Hypostomini (suckermouth... 相似文献
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Parathyroidectomy Improves Survival In Patients with Severe Hyperparathyroidism: A Comparative Study
Patricia Taschner Goldenstein Rosilene Motta Elias Lilian Pires de Freitas do Carmo Fernanda Oliveira Coelho Luciene Pereira Magalh?es Gisele Lins Antunes Melani Ribeiro Custódio Fábio Luiz de Menezes Montenegro Silvia Maria Titan Vanda Jorgetti Rosa Maria Affonso Moysés 《PloS one》2013,8(8)
Background and objectives
Secondary hyperparathyroidism (SHPT) in CKD is associated with an increased risk for mortality, but definitive data showing that parathormone control decreases mortality is still lacking. This study aimed to compare the mortality of patients with severe SHPT submitted to parathyroidectomy(PTX) with those who did not have access to surgery.Methods
This is a retrospective study in a cohort of 251 CKD patients with severe SHPT who were referred to a CKD-MBD Center for PTX from 2005 until 2012.Results
Most of our patients had indication of PTX, but only 49% of them had access to this surgical procedure. After a mean follow-up of 23 months, 72 patients had died. Non-survivors were older; more often had diabetes, lower serum 25 vitamin D and mostly had not been submitted to surgery. The relative risk of death was lower in the PTX patients (0.428; 95% CI, 0.28 to 0.67; p<0.0001). After adjustments, mortality risk was dependent on age (1.04; 95% CI, 1.01 to 1.07; p = 0.002), 25 vitamin D (0.43; 95% CI, 0.24 to 0.81; p = 0.006) and no access to PTX (4.13; 95% CI, 2.16 to 7.88; p<0.0001). Results remained the same in a second model using the PTX date as the study start date for the PTX group.Conclusions
Our data confirms the benefit of PTX on mortality in patients with severe SHPT. The high mortality encountered in our population is significant and urges the need to better treat these patients. 相似文献5.
Marcelo Moura Linhares Renato José Affonso Jr Luciano de Souza Viana Sandra Regina Morini Silva Marcos Vinicius Araujo Denadai Silvia Regina Caminada de Toledo Delcio Matos 《PloS one》2015,10(12)
Objective
To evaluate the relationship between the expression profiles of 84 extracellular matrix (ECM) genes and the prognosis of patients with colorectal cancer (CRC).Methods
This retrospective study included 114 patients with stage I–IV CRC who underwent primary tumour resection. Quantitative real-time PCR and immunohistochemistry assays were conducted using primary tumour samples. Kaplan-Meier survival curves were also generated to identify differences in global survival (GS) and disease-free survival (DFS) for the hypo- or hyperexpression status of each marker. The log-rank test was used to verify whether the differences were significant. Stepwise Cox regression models were also used to identify the risk factors associated with GS and DFS in a multivariate mode, and then were used to score the risk of death associated with each marker, either independently or in association.Results
In the univariate analyses, significant differences in GS in relation to the expression profiles of ITGAV (p = 0.001), ITGA3 (p = 0.002), ITGA6 (p = 0.001), SPARC (p = 0.036), MMP9 (p = 0.034), and MMP16 (p = 0.038) were observed. For DFS, significant differences were observed in associated with ITGAV (p = 0.004) and ITGA3 (p = 0.001). However, only the ITGAV and ITGA6 gene markers for GS (hazard ratio (HR) = 3.209, 95% confidence interval (CI) = 1.412–7.293, p = 0.005 and HR = 3.105, 95% CI = 1.367–7.055, p = 0.007, respectively), and ITGA3 for DFS (HR = 3.806, 95% CI = 1.573–9.209, p = 0.003), remained in the final Cox regression models. A scoring system was developed to evaluate the risk of patient death based on the number of markers for the components of the final GS model. Scores of 0, 1, or 2 were associated with the following mean survival rates [CI]: 47.162 [44.613–49.711], 39.717 [35.471–43.964], 30.197 [24.030–36.327], respectively.Conclusions
Multivariate mathematical models demonstrated an association between hyperexpression of the ITGAV and ITGA6 integrins and GS, and also between the ITGA3 integrin and DFS, in patients with colorectal tumours. A risk scoring system based on detected hyperexpression of 0, 1, or 2 markers (e.g., ITGAV and/or ITGA6) was also found to accurately correlate with the GS curves generated for the present cohort. 相似文献6.
Jaqueline Inês Alves de Andrade Eduardo Akifumi Ono Glauber Cruz de Menezes Elenice Martins Brasil Rodrigo Roubach Elisabeth Criscuolo Urbinati Marcos Tavares-Dias Jaydione Luiz Marcon Elizabeth Gusmo Affonso 《Comparative biochemistry and physiology. Part A, Molecular & integrative physiology》2007,146(4):576
This study evaluated the influence of diets supplemented with 500, 800, 1200 mg kg− 1 of vitamin C (ascorbic acid or AA) and vitamin E (α-tocopherol or α-T) on the physiological responses of pirarucu fed for 2 months. Weight and mortality were not affected by dietary vitamin type or their concentrations. Significant increase (p < 0.05) on the red blood cells count was obtained on treatments with 800 and 1200 mg AA kg− 1 and on the hemoglobin concentration on treatment with 500 mg α-T kg− 1 relatively to control. Mean corpuscular volume presented a significant decrease (p < 0.05) on treatment with 800 and 1200 mg AA kg−1 when compared to control. Mean corpuscular hemoglobin concentration was significantly high (p < 0.05) on treatment with 500 mg α-T kg− 1. Only in vitamin C treatments, we noticed a significant increase (p < 0.05) in the number of leucocytes relative to control. All fish in the vitamin-supplemented treatments, except 500 mg AA kg− 1, had high total protein values compared to control. Fish treated with 800 or 1200 mg α-T kg− 1 also showed increases in plasma glucose concentrations. Our results suggest that 800 and 1200 mg AA kg− 1 are probably the most suitable concentrations for pirarucu diets, although high vitamin E diets are not necessary for quantitative leucocyte increases for this species. 相似文献
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The genus Centropyge is remarkable for species richness, composing a highly specialized fish group amongst members from family Pomacanthidae. However, cytogenetical reports are nearly absent in these animals. New data are provided from karyotypical studies carried out on Centropyge aurantonotus from the Brazilian coast of the Atlantic Ocean and C. ferrugatus from the Philippines Sea of the Indo-Pacific Ocean. Both species present 2n=48 but karyotypes are differentiated by fundamental number. C. aurantonotus has a great number of biarmed chromosomes (4 m + 14 sm+16 st+4 a), while C. ferrugatus presents only acrocentric chromosomes. Single nucleolar organizer regions (NORs) are located at interstitial position of an acrocentric pair in C. ferrugatus and on short arms of a subtelocentric pair in C. aurantonotus, as confirmed by fluorescent in situ hybridization (FISH) with 18S rDNA probes. Heterochromatin is distributed over NOR and centromeric regions in both species, but additional GC-rich heterochromatic blocks on short arms of up to eight chromosomal pairs can be detected in C. aurantonotus. 5S rDNA segments were located interstitially on two chromosomal pairs in C. ferrugatus and on nine pairs in C. aurantonotus, mostly equivalent to heterochromatic blocks on short arms of biarmed chromosomes. C. ferrugatus can be considered a species in which basal chromosomal features proposed for modern Teleosteans were conserved. The derived karyotype pattern of C. aurantonotus seems to be determined by pericentric inversions and heterochromatin addition which probably determined the notorious dispersion of 5S rRNA (pseudo)genes. It is demonstrated that, even within a group generally characterized by cytogenetical homogeneity as the family Pomacanthidae, diversified karyotypes can be found. 相似文献
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Aline Souza Medrado Mavione Souza Ribeiro Paulo Roberto Antunes de Mello Affonso Paulo Luíz Souza Carneiro Marco Ant?nio Costa 《Genetics and molecular biology》2012,35(4):797-801
The fish genus Astyanax is widespread throughout the Neotropical region and is one of the most species-rich genera of the Characiformes. Cytogenetic studies of Astyanax have revealed marked intra- and interspecific diversity, with the identification of various species complexes. In this report, we describe the karyotypic structure of two sympatric species of Astyanax (Astyanax sp. and Astyanax aff. fasciatus) from the Middle Contas River basin in the northeastern Brazilian state of Bahia. Both species had 2n = 48 but differed in their karyotypic formulae. Small heterochromatic blocks and multiple nucleolar organizer regions (NORs) were identified in both species. Terminal CMA3+/DAPI− signals were observed in Astyanax sp. and A. aff. fasciatus, mostly coincident with NORs. These results show that chromosomal markers can be used to identify species in this fish complex. These markers can provide useful information for evolutionary studies and investigations on the mechanisms of chromosomal diversity in Astyanax. 相似文献
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Igor de Paiva Affonso Luiz Carlos Gomes Angelo Antonio Agostinho Hugo José Message João Dirço Latini Emili García-Berthou 《Reviews in Fish Biology and Fisheries》2016,26(1):71-81
Understanding the variation of fish assemblages in reservoirs is crucial for precise interpretations of ecological processes. However, representative samples in such environments are subjected both to spatial zonation (longitudinal and transversal gradients) and limitations of fishing gears. We used a large zoned reservoir (Itaipu, Brazil) to perform a quasi-experiment that assessed the relative role of spatial gradients and fishing methods on fish diversity and composition. Active (cast nets, electrofishing and seines) and passive (gillnets and longlines) fishing gears were employed in lotic, transitional and lentic habitats of the reservoir and three large tributaries. In total 6281 individuals of 101 species were captured. Sampling methods and spatial gradients had an influence on species composition, with significant interactions between these factors. In addition, results produced by a variation partitioning analysis suggested that 21 % of the species richness was explained by the sampling methods and 7 % by the longitudinal gradient; whereas for species composition, 12 and 3 % corresponded to sampling methods and transversal gradients, respectively. Overall, our results suggest that variations among fishing gears is greater than across strong spatial gradients, emphasizing the importance of using multiple and complementary fishing methods in reservoirs for characterization of fish assemblages. The interactions between spatial gradients and sampling methods were higher with transversal than with longitudinal gradients, suggesting that substantial differences in species richness and composition among sampling gears depend to some extent on habitat. The approach used here would be applicable to any large zoned inland waterscape. 相似文献