全文获取类型
收费全文 | 8567篇 |
免费 | 794篇 |
国内免费 | 1篇 |
出版年
2021年 | 162篇 |
2020年 | 86篇 |
2019年 | 108篇 |
2018年 | 152篇 |
2017年 | 120篇 |
2016年 | 203篇 |
2015年 | 331篇 |
2014年 | 360篇 |
2013年 | 477篇 |
2012年 | 532篇 |
2011年 | 515篇 |
2010年 | 349篇 |
2009年 | 325篇 |
2008年 | 448篇 |
2007年 | 498篇 |
2006年 | 416篇 |
2005年 | 353篇 |
2004年 | 352篇 |
2003年 | 346篇 |
2002年 | 281篇 |
2001年 | 190篇 |
2000年 | 171篇 |
1999年 | 165篇 |
1998年 | 123篇 |
1997年 | 83篇 |
1996年 | 85篇 |
1995年 | 93篇 |
1994年 | 73篇 |
1993年 | 78篇 |
1992年 | 123篇 |
1991年 | 95篇 |
1990年 | 134篇 |
1989年 | 86篇 |
1988年 | 74篇 |
1987年 | 84篇 |
1986年 | 73篇 |
1985年 | 76篇 |
1984年 | 65篇 |
1983年 | 65篇 |
1982年 | 63篇 |
1981年 | 58篇 |
1980年 | 50篇 |
1979年 | 64篇 |
1977年 | 48篇 |
1976年 | 48篇 |
1975年 | 38篇 |
1974年 | 45篇 |
1973年 | 37篇 |
1972年 | 41篇 |
1971年 | 41篇 |
排序方式: 共有9362条查询结果,搜索用时 15 毫秒
1.
Christopher T. Clarke 《Restoration Ecology》1997,5(3):256-264
No guidelines are currently available that advise which soil properties of arable land can be used to suggest suitable locations for heathland reconstruction. This paper reviews studies comparing soil properties of heathland or semi-natural grassland with those of adjacent arable fields, investigations in the autecology of the dominant heathland plant, Calluna vulgaris (common heather), and long-term experiments of fertilizer inputs on arable soils. Three properties must be assessed before the suitability of a field can be determined: extractable phosphorus, exchangeable calcium, and pH. A number of other nutrients may also be important, but evidence is currently insufficient to substantiate this. Natural changes in levels of extractable phosphorus, exchangeable calcium, and pH appear to be very slow, so nutrient stripping and acidification will be necessary where recommended levels are exceeded to successfully restore heathland vegetation. 相似文献
2.
3.
4.
Paul D Clarke 《BMJ (Clinical research ed.)》1980,281(6256):1704-1705
5.
6.
7.
This study presents an approach to identifying surface residues on membrane proteins that are exposed toward the membrane-aqueous interface. The method employs a lipid Ni(II) chelate that localizes the metal ion to a region near the membrane-aqueous interface. Lateral diffusion of the lipid chelate results in Heisenberg exchange (HE) with nitroxide side chains in the protein only if direct contact occurs between the paramagnetic species during a collision. Thus, HE serves as a signature for residues facing the bilayer in the neighborhood of the membrane-aqueous interface. To evaluate the method, 13 surface residues on the extracellular half of KcsA, a prokaryotic potassium channel of known structure, were examined for HE with the Ni(II) chelate. The HE rate between the two species is found to depend strongly on the vertical position of the nitroxide with respect to the membrane-aqueous interface. Nitroxides introduced near the interface experience relatively high HE rates, whereas nitroxides that are immersed in the bilayer interior or sterically sheltered from collision experience low or undetectable rates. The results indicate that residues near the interface can be identified on the basis of their high rates of collision with the headgroup region of the bilayer. 相似文献
8.
9.
10.
Rapid degradation of D- and L-succinimide-containing peptides by a post-proline endopeptidase from human erythrocytes 总被引:1,自引:0,他引:1
We have been interested in the metabolic fate of proteins containing aspartyl succinimide (Asu) residues. These residues can be derived from the spontaneous rearrangement of Asp and Asn residues and from the spontaneous demethylation of enzymatically methylated L-isoAsp and D-Asp residues. Incubation of the synthetic hexapeptide N-Ac-Val-Tyr-Pro-Asu-Gly-Ala with the cytosolic fraction of human erythrocytes resulted in rapid cleavage of the prolyl-aspartyl succinimide bond producing the tripeptide N-Ac-Val-Tyr-Pro. The rate of this reaction is equal for both L- and D-Asu-containing peptides and is 10-fold greater than the rate of cleavage of a corresponding peptide containing a normal Pro-Asp linkage. When the aspartyl succinimide ring was replaced with an isoaspartyl residue, the cleavage rate was about 5 times that of the normal Pro-Asp peptide. The tripeptide-producing activity copurified on DEAE-cellulose chromatography with an activity that cleaves N-carbobenzoxy-Gly-Pro-4-methylcoumarin-7-amide, a post-proline endopeptidase substrate. These two activities were both inhibited by an antiserum to rat brain post-proline endopeptidase, and it appears that they are catalyzed by the same enzyme. This enzyme has a molecular weight of approximately 80,000 and is covalently labeled and inhibited by [3H]diisopropyl fluorophosphate. The facile cleavage of the succinimide- and isoaspartyl-containing peptides by this post-proline endopeptidase suggests that it may play a role in the metabolism of peptides containing altered aspartyl residues. 相似文献