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1.
Ghulam Jilani Abida Akram Raja M. Ali Fauzia Y. Hafeez Imran H. Shamsi Arshad N. Chaudhry Abid G. Chaudhry 《Annals of microbiology》2007,57(2):177-184
Field experiment was conducted on fodder maize to explore the potential of integrated use of chemical, organic and biofertilizers for improving maize growth, beneficial microflora in the rhizosphere and the economic returns. The treatments were designed to make comparison of NPK fertilizer with different combinations of half dose of NP with organic and biofertilizers viz. biological potassium fertilizer (BPF), Biopower, effective microorganisms (EM) and green force compost (GFC). Data reflected maximum crop growth in terms of plant height, leaf area and fresh biomass with the treatment of full NPK; and it was followed by BPF+full NP. The highest uptake of NPK nutrients by crop was recorded as: N under half NP+Biopower; P in BPF+full NP; and K from full NPK. The rhizosphere microflora enumeration revealed that Biopower+EM applied along with half dose of GFC soil conditioner (SC) or NP fertilizer gave the highest count of N-fixing bacteria (Azotobacter, Azospirillum, Azoarcus andZoogloea). Regarding the P-solubilizing bacteria,Bacillus was having maximum population with Biopower+BPF+half NP, andPseudomonas under Biopower+EM+half NP treatment. It was concluded that integration of half dose of NP fertilizer with Biopower+BPF / EM can give similar crop yield as with full rate of NP fertilizer; and through reduced use of fertilizers the production cost is minimized and the net return maximized. However, the integration of half dose of NP fertilizer with biofertilizers and compost did not give maize fodder growth and yield comparable to that from full dose of NPK fertilizers. 相似文献
2.
Chokri A El Abida K Zegzouti YF Ben Cheikh R 《Canadian journal of physiology and pharmacology》2012,90(5):607-616
The vasodilatory effect of Globularia alypum L. (GA) extract was evaluated in rat mesenteric arterial bed pre-contracted by continuous infusion of phenylephrine (2-4 ng/mL). Bolus injections of GA elicited dose-response vasodilation, which was abolished after endothelium removal. Addition of a nitric oxide synthase inhibitor, N(G)-nitro-l-arginine methyl ester (100 μmol/L), alone or in the presence of a cyclooxygenase inhibitor, indomethacin (10 μmol/L), did not significantly affect the vasodilation of the mesenteric arterial bed in response to GA extract. These results suggest that GA-induced vasodilation is endothelium dependent but nitric oxide and prostacyclin independent. In the presence of high K(+) (60 mmol/L), the GA vasodilatory effect was completely abolished, suggesting that the vasodilation effect is mediated by hyperpolarization of the vascular cells. Also, pre-treatment with atropine (a muscarinic receptors antagonist) antagonized the GA-induced vasodilation, suggesting that the vasodilatory effect is mainly mediated by the endothelium-derived hyperpolarizing factor through activation of endothelial muscarinic receptors. 相似文献
3.
Olfa Abida Riadh Ben Mansour Bochra Gargouri Mourad Ben Ayed Abderrahmen Masmoudi Hamida Turki Hatem Masmoudi Saloua Lassoued 《Biological trace element research》2012,150(1-3):74-80
Pemphigus is an autoimmune disorder resulting from the interaction between autoantibodies and desmoglein. Oxidative stress seems to be responsible for the onset/aggravation of many human diseases. Actually, it is considered as one of the several factors for the etiopathogenesis of pemphigus. The present study aims to evaluate the oxidative state in the sera of pemphigus vulgaris and pemphigus foliaceus patients by assessing lipid peroxidation, proteins oxidation, and antioxidant enzyme activity. This study included 36 pemphigus vulgaris and 42 pemphigus foliaceus patients as well as a group of controls consisting of 78 healthy volunteers. Malondialdehyde levels (p?<?0.001) and catalase activity (p?<?0.001) are higher in both groups of patients than in the control group. The two groups of patients showed a nonsignificant decrease in the thiol groups compared with the healthy one. A nonsignificant difference was shown between pemphigus vulgaris and pemphigus foliaceus patients, except for the catalase which shows an increase in the pemphigus vulgaris group. We have also found significant correlations between serum oxidative stress marker levels and serum anti-desmoglein antibody levels in the two pemphigus groups. These findings underline the implication of oxidative stress in the physiopathology of pemphigus by the increase in the autoantibodies?? reactivity. 相似文献
4.
Shaista Akhund Abida Akram Nafeesa Qudsia Hanif Rahmatullah Qureshi Farah Naz Brian Gagosh Nayyar 《Mycotoxin Research》2017,33(2):147-155
Various cultivars of red chilli were collected from a small town named Kunri, located in the province Sindh, Pakistan. This town is a hub of red chilli production in Asia. A total of 69 samples belonging to 6 cultivars were obtained and analysed for the occurrence of aflatoxins and Aspergillus flavus, to explore the potential of resistant and susceptible germplasm. Aflatoxins were detected by thin layer chromatography (TLC) and high performance liquid chromatography (HPLC), while A. flavus was isolated and identified using agar plate, blotter paper, deep freezing and dilution techniques. Molecular characterization using internal transcribed spacer (ITS) 1/4 and A. flavus specific FL1-F/R primers confirmed the identity of A. flavus. The data revealed that 67 and 75% samples contaminated with aflatoxin B1 (AFB1) and with A. flavus, respectively. A highly susceptible chilli cultivar was ‘Nagina’, showing 78.8% frequency of total aflatoxins (1.2–600 μg/kg) and a mean of 87.7 μg/kg for AFB1 and 121.9 μg/kg for total aflatoxins. A. flavus was detected with 93% frequency and 2.14 × 104 colony forming units. In contrast, cultivars ‘Kunri’ and ‘Drooping Type’ were found to be resistant, with low levels of aflatoxins and fungal counts. The study was conducted for the first time to explore two potential cultivars that were less susceptible towards A. flavus and aflatoxin contamination. These cultivars could be preferably cultivated and thereby boost Pakistan’s chilli production. 相似文献
5.
An improved protocol for Agrobacterium tumefaciens-mediated transformation and regeneration of the tomato cultivar Rio Grande with Arabidopsis thaliana early flowering genes AP1 and LFY has been developed. The effect of different factors on the efficiency of regeneration as well as transformation has been
studied. A. tumefaciens strain EHA 105 harboring either of the binary vectors pROKIIAP1GUSint or pROKIILFYGUSint, containing APETALA 1, LEAFY (LFY), β-glucuronidase (GUS), and neomycin phosphotransferase II (NPTII) was used for transformation. The effect of different regeneration media was studied and Murashige and Skoog (MS) medium
containing Zeatin 1 mg/l and IAA 0.5 mg/l was found best, producing the highest percentage of regeneration, i.e., 30.4% and
transformation, i.e., 84% (P < 0.05). The age of explants was studied and 1-week-old explants were found best producing highest regeneration (29.5%) and
transformation (87.5%) values (P < 0.05). Three different types of explants were compared and best results were obtained from apical meristem explants (60.5%
regeneration and 85% transformation) (P < 0.05). Transient and stable GUS expression was observed in the transgenic plants. 相似文献
6.
Salsolinol (1-methyl-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline; Sal) is structurally similar to 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine,
which is supposed to have a role in the development of Parkinson-like syndrome in both human and non-human subjects. In the
human brain, the amount of (R)-enantiomer of Sal is much higher than (S)-enantiomer, suggesting that a putative enzyme may participate in the synthesis of (R)-salsolinol, called (R)-salsolinol synthase. In this study, the (R)-salsolinol synthase activity in the condensation of dopamine and acetaldehyde was investigated in the crude extracts from
the brains of Sprague Dawley rats. Identification of the enzymatic reaction products and enzyme activity detection were achieved
by HPLC-electrochemical detection. The discovery of this enzyme activity in rat’s brain indicates the natural existence of
(R)-salsolinol synthase in the brains of humans and rats, and it is distributed in most brain regions of rat with higher activity
in soluble proteins extracted from striatum and substantia nigra. 相似文献
7.
Ovais Muhammad Ahmad Irshad Khalil Ali Talha Mukherjee Sudip Javed Rabia Ayaz Muhammad Raza Abida Shinwari Zabta Khan 《Applied microbiology and biotechnology》2018,102(10):4305-4318
Applied Microbiology and Biotechnology - Nanotechnology has emerged as a prominent scientific discipline in the technological revolution of this millennium. The scientific community has focused on... 相似文献
8.
Jamshed Iqbal Mohammed I. El-Gamal Syeda Abida Ejaz Joanna Lecka Jean Sévigny 《Journal of enzyme inhibition and medicinal chemistry》2018,33(1):479-484
Tissue-nonspecific alkaline phosphatase (TNAP) is an important isozyme of alkaline phosphatases, which plays different pivotal roles within the human body. Most importantly, it is responsible for maintaining the balanced ratio of phosphate and inorganic pyrophosphate, thus regulates the extracellular matrix calcification during bone formation and growth. The elevated level of TNAP has been linked to vascular calcification and end-stage renal diseases. Consequently, there is a need to search for highly potent and selective inhibitors of alkaline phosphatases (APs) for treatment of disorders associated with the over-expression of APs. Herein, a series of tricyclic coumarin sulphonate 1a-za with known antiproliferative activity, was evaluated for AP inhibition against human tissue nonspecific alkaline phosphatase (h-TNAP) and human intestinal alkaline phosphatase (h-IAP). The methylbenzenesulphonate derivative 1f (IC50?=?0.38?±?0.01?μM) was found to be the most active h-TNAP inhibitor. Another 4-fluorobenzenesulphonate derivative 1i (IC50?=?0.45?±?0.02?μM) was found as the strongest inhibitor of h-IAP. Some of the derivatives were also identified as highly selective inhibitors of APs. Detailed structure-activity relationship (SAR) was investigated to identify the functional groups responsible for the effective inhibition of AP isozymes. The study was also supported by the docking studies to rationalise the most possible binding site interactions of the identified inhibitors with the targeted enzymes. 相似文献
9.
Distinctive inhibition of alkaline phosphatase isozymes by thiazol‐2‐ylidene‐benzamide derivatives: Functional insights into their anticancer role 下载免费PDF全文
10.
Ovais Muhammad Khalil Ali Talha Raza Abida Islam Nazar Ul Ayaz Muhammad Saravanan Muthupandian Ali Muhammad Ahmad Irshad Shahid Muhammad Shinwari Zabta Khan 《Applied microbiology and biotechnology》2018,102(10):4393-4408
Applied Microbiology and Biotechnology - Phytochemicals offer immense promise for sustainable development and production of nanotechnology-enabled products. In the present study, Olax nana Wall. ex... 相似文献