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1.
Interactions of Vesicular Stomatitis Virus Structural Proteins with HeLa Plasma Membranes 总被引:22,自引:0,他引:22
THE processes whereby nucleoprotein core particles of certain animal viruses become enveloped by and bud off from host cell membranes can be studied by preparing membrane1,2 or “sedimentable”3 fractions from infected cells and examining them for the presence of virus proteins. We find that similar experiments designed to monitor assembly of vesicular stoma-titus virus (VSV) at sites along the plasma membranes of HeLa cells are best interpreted after first investigating the possibility that virus proteins adsorb to plasma membranes during cell fractionation and membrane isolation. In this report, we show that at 0° C the membrane protein of VSV, among other virus proteins, adsorbs to plasma membranes isolated from uninfected HeLa cells. With appropriate pulse-chase experiments, however, we are able to demonstrate the progressive association, in vivo, of VSV core protein with plasma membranes of infected HeLa cells. 相似文献
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Morphological and physiological characteristics of micropropagatedplants of Delphinium cv. Princess Caroline were studied. Leavesproduced in vitro showed poor control of water loss which appearsto result from restricted responses by stomata and not frompoor cuticular development. Stomata of leaves produced in vitrowere larger and more frequent than those produced during acclimatization.Despite the fact that stomata from isolated epidermis of leavesproduced in vitro reduced their apertures when exposed to turgor-reducingtreatments, they did not close fully. This, together with highstomatal frequencies might explain the poor control of waterloss shown by intact leaves produced in culture when exposedto dry air. While leaves from acclimatized plants showed almostcomplete closure with ABA, low water potentials, darkness andCO2, stomata from leaves produced in vitro reduced their apertureswhen exposed to those factors, but only to a limit. Therefore,stomata from leaves cultured in vitro seem to be partially functional,but some physiological or anatomical alteration prevents themfrom closing fully. Stomata from leaves produced in vitro wereparticularly insensitive to ABA which appears to be partly associatedwith the high cytokinin concentration in the culture medium.In the long-term, this stomatal insensitivity to ABA might contributeto plant losses when micropropagated plantlets are transferredto soil. Key words: Micropropagation, stomatal physiology, dehydration, PEG, ABA, BAP, darkness, CO2, Delphinium 相似文献
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As the history of science has developed as a professional intellectualdiscipline, it has had and will continue to have an importantrole in defining science and its place in our culture. Suchdefinitions should be based on as much information as possible.Scientists can help supply some of this information throughparticipation in symposia on the history of science. In addition,scientists can learn much about the nature of their disciplineby becoming aware of the concepts of science which are derivedfrom the careful analysis of its history. Efforts should bemade to bring historians of science and scientists togetherfor their mutual benefit. 相似文献
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Posterior probabilities for choosing a regression model 总被引:4,自引:0,他引:4
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PHILIP W. ATKINSON ALLAN J. BAKER KAREN A. BENNETT NIGEL A. CLARK JACQUIE A. CLARK KIMBERLY B. COLE ANNE DEKINGA AMANDA DEY SIMON GILLINGS PATRICIA M. GONZÁLEZ KEVIN KALASZ CLIVE D. T. MINTON JASON NEWTON LAWRENCE J. NILES THEUNIS PIERSMA ROBERT A. ROBINSON HUMPHREY P. SITTERS 《Journal of Applied Ecology》2007,44(4):885-895