乙型肝炎病毒全基因序列的测定

Seven cases of chronic hepatitis B virus carriers were included for sequencing of whole gene sequences of hepatitis B virus (HBV). The serotype of 4 strains of HBV were adr, and 3 strains were adw. Two strains were classified to genotype B, and the other 5 strains to genotype C. No significant mutations, such as A¹⁸⁹⁶, T¹⁷⁶²A¹⁷⁶⁴ were present. With other reported 2 complete sequences of HBV strains prevailing in mainland China, 7 strains of HBV whole sequences of genotype C from mainland China were analyzed to produce the consensus sequence of HBV of China. Comparing of the consensus sequence with that from Genbank, there were only 22 sites with different nucleotides.     

乙型肝炎病毒全基因序列的测定

测定７ 例慢性 Ｈ Ｂ Ｖ 携带者 Ｈ Ｂ Ｖ 基因组全序列,经同源性比较,确定基因型。２ 例基因型为 Ｂ 型,余均为 Ｃ 型;血清型ａｄｒ ４ 例,ａｄｗ ３ 例。各序列间 Ｘ 基因差异最大。未见 Ａ１８９６ 、 Ｔ１７６２ Ａ１７６４等重要位点的变异。结合已有的２ 株 Ｈ Ｂ Ｖ 中国流行株全基因序列,初步建立以中国流行株序列为基础的 Ｈ Ｂ Ｖ 标准序列,该标准序列与国外标准序列仅有２２ 个位点的差异     

一例中国重型肝炎病人乙型肝炎病毒基因组全序列的测定

用聚合酶链反应（PCR）产物直接和克隆后序列分析，测定1例重型肝炎病人感染的HBV变异毒株全基因组序列，该毒株长3221个碱基，为adw亚型，与同一地区HfuAg阳性无症状携带者感染的adw亚型全序列比较，有35个罕见和独特的核着酸改变，导致27个氨基酸替代，其中前C和C基因变异最多，这一毒株在多种调节序列，包括启动子SPI、SP11、XP、增强子ENI和EN11，也存在变异。但并无日本毒株的X区和ENHll－CP的聚集变异。结果说明我国重肝HBV毒株有独特的变异特点。