依赖粪便材料的大熊猫肠道耶尔森氏菌的检测

It is inevitable to develop noninvasive sampling methods to do studies on giant panda even diagnose the diseases since which is so endangered that it's impossible to carry out invasive sampling. A non-invasive sampling method to detect the intestinal pathogen, Yersirda enterocolitica in feces of pandas based designing PCR primers was established in this study. The main procedures are based on bacteria enrichment and cell lysis before binding the pathogen DNA to silica powder at high concentration of Kalium iodide and neutral pH conditions. Before PCR cycles, the binded DNA is washed with 80% ethanol and eluted with diluted EDTA buffer. Restdts showed that the silica-based feces DNA-purification method could remove the inhibitors of PCR so applicable to detect the target pathogen.     

药饮同源野菊花

每年金秋,野外田间地头、沟畔崖底、近水洼地,常看到簇簇黄色小花,顶在茎头,这就是野菊花。野菊花来源于菊科植物野菊(Chrysanthemum indicum)、岩香菊(Ch.lavandulaefolium)、甘野菊(Ch.boreale)的干燥头状花序,又称疟疾草、苦薏、山菊花、菊花脑等,可疏风清热、消肿解毒,用于治疗流感、流脑、呼吸道感染、高血压、肝炎及痢疾等症;外敷可治疔疮、热疖、